Haematological changes associated with Newcastle disease vaccination in chickens using gums from Cedrela odorata and Khaya senegalensis as delivery agents


Our previous ex-vivo and in vivo investigations have established immunopotentiating property of Khaya senegalensis and Cedrela odorata gums; however the safety of the use of this gum combination in chicken has not been described. Hence this study evaluates the haematological profile of chickens vaccinated with Newcastle disease vaccine delivered through the oral and ocular routes using gums from Cedrela odorata and Khaya senegalensis as delivery agents. 252 one-day old chickens were grouped gum-vaccine oral (GVOR), vaccine oral (VOR), gum-vaccine ocular (GVOC), vaccine ocular (VOC), gum oral (GOR), gum ocular (GOC), no-gum-no-vaccine but challenged (NGNV/C), no-gum-no-vaccine unchallenged (NGNV/U). They were vaccinated on days 21 and 42 and challenged day 84. Blood samples were collected before first vaccination and at selected intervals afterwards. Analysis was done using one way ANOVA with P<0.05 considered significant. Packed cell volume, total white cell count, heterocyte-lymphocyte ratios and platelet count varied insignificantly (P>0.05) throughout the period of observation across groups with no observable derangements. Hence, the absence of derangement in haematological indices from this study suggests that the dilution rate recommended from the ex-vivo study is safe for administration of Newcastle disease vaccine in chickens irrespective of the routes of delivery

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Alexander, D.J. (2000). Newcastle disease and other avian paramyxoviruses. Rev. Sci. tech. Off. Int. Epiz, 19(2), 443-462

Benjamin, M.M. (1978). Outline of Veterinary Clinical Pathology. Iowa State University Press, Ames, Iowa, USA. Pp 25-58, 103-104.

Emikpe, B.O., Oyebanji, V.O., Odeniyi, M.A., Salaam, A.M., Oladele, O.A., Jarikre, T.A., and Akinboade, OA. (2016). Ex-vivo evaluation of the mucoadhesive properties of Cedrela odorata and Khaya senegalensis gums with possible applications for veterinary vaccine delivery. SpringerPlus (5): 1289. doi:10.1186/s40064-016-2948-0.

Ingale, A.G, and Hivrale, AU. (2013). Plant as a plenteous reserve of lectin-a review. Plant Signal Behav. Dec; 8(12): e26595.

Jain, N.C. (1986). Schalm’s Veterinary Haematology, 4th ed. Lea and Febriger Philadelphia. Pp.32-35.

Kuku, A., Agboola, F., and Aboderin, A. (2005). Purification and Characterisation of a Lectin from the seeds of Psophocarpus palustris. Pak. J. Biol. Sci., 8 (12): 1667-1671.

Okwor, E.C, Eze, D.C. (2010). The annual prevalence of Newcastle disease in commercial chickens reared in South Eastern Savannah zone of Nigeria. Res. J.Poult. Sci. 3: 23-26.

Okwor, E.C., Eze, D.C. and Uzuegbu, M.O. (2009). Effect of storage condition on the potency of Newcastle disease vaccine la sota. Int. J. Poult. Sci., (8); 999-1002.

Oyebanji, V.O., Emikpe, B.O., Oladele, O, Odeniyi, MO, Salami, A, Osowole, O.I., Kasali, O.B., and Akinboade, O.A. (2016) Evaluation of immune response in challenged chickens administered with Newcastle disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agents. J. of immunoas. and Immunochem. http://dx. doi.org/10.1080/ 153 21819 .2016 .1273237

Susuki, M, Takatsuki, F., Maeda, Y.Y., Hamuro, J, et al. (1994). Lentinan-rationale for development and therapeutic potential. Clin. Immunother.; 2: 121–5.

Torky, Z.A. (2016). Antiviral Activity of Euphorbia Lectin Against Herpes Simplex Virus 1 and its Antiproliferative Activity Against Human Cancer Cell-Line. J Antivir Antiretrovir 8:107-116. doi: 10.4172/1948-5964.1000142.

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