Cement Dust Exposure Alters Secretory Functions, Biochemical Profile and Morphology of Gastrointestinal Tissues in Rats Exposed to Cement Dust
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Owonikoko, M. W., salami, A. T., Odukanmi, O. A., Emikpe, B. O., & Olaleye, S. B. (2022). Cement Dust Exposure Alters Secretory Functions, Biochemical Profile and Morphology of Gastrointestinal Tissues in Rats Exposed to Cement Dust. Nigerian Journal of Physiological Sciences, 37(2), 185–194. https://doi.org/10.54548/njps.v37i2.4


Asides direct gastrointestinal exposure, inhalation route is another major xenobiotic exposure pathway to the gastrointestinal tract via mucociliary escalator. This triphasic study assesses cement dust inhalatory exposure effect on the possible alterations of the gastrointestinal tissues and secretion. 72 male, sixteen (16) weeks old Wistar rats were randomized into 3 different phases of 24 animals. Each phase comprised of 3 group of 8 animals. Group 1 (control) were sham-operated with clean ambient air, group 2 (14-days exposed) were exposed to cement dust for 14days, and group 3 (28-day exposed) were exposed to cement dust for 28 days. Biochemical indices including superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), sulfhydryl group, carbonyl group, Na+-K+ATPase pump activity, Nitric oxide (NO) were investigated spectrophotometrically in gastric and hepatic tissues while histopathology was studied using standard procedure. There was significant increase in the level of MDA, NO and carbonyl- an observation that contrasts with the level of CAT, SOD and sulfhydryl; no significant difference in Na+-K+-ATPase pump was observed in the exposed groups compared with control. Histopathological alterations in salivary gland and gastric tissues includes edema, inflammatory cell infiltration and vascular congestion. There was significant alteration in basal salivary, gastric and biliary secretions; increased stimulated salivary and gastric secretion via cholinergic stimulation. Conclusively, histopathological and spectrophotometric analyses reflect that inhalatory experimental exposure to cement dust significantly alter gastrointestinal secretions and predisposes the gastrointestinal tract to an array of deleterious effects via protein oxidation and antioxidant depletion and tissue peroxidation.

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Adak, M. D., Adak, S., Purohit, K.M. 2000. Ambient air quality and health hazards near min-cement plants. Pollution Research. 26. 3: 361- 364

Amure, B. O. Ginsburg, M. 1964. Inhibitors of histamine catabolism and the action of gastrin in the rat. British Journal of Pharmacology and Chemotherapy. Volume23, Issue3 Pages 476-485. https://doi.org/10.1111/j.1476-5381.1964.tb01604.x

Aydin, S., Aydin, S., Croteau, G., I. Sahin, I and Citil, C. 2010. "Ghrelin, Nitrite and Paraoxonase/Arylesterase Concentrations in Cement Plant Workers. Journal of Medical Biochemistry. 29, (2): 78-83.

Breusing, N., and Grune, T. 2010. Biomarkers of protein oxidation from a chemical, biological and medical point of view. Exp. Gerontol. 45 (10) (2010)733–737, http: //dx.doi.org/10.1016/j.exger.2010.04.00420403419

Brzóska M. M., Moniuszko-Jakoniuk, J., Pilat-Marcinkiewicz, B. and Sawicki, B. 2003. Liver and Kidney Function and Histology in Rats Exposed to Cadmium and Ethanol. Alcohol & Alcoholism Vol. 38, No. 1, pp. 2–10 doi:10.1093/alcalc/agg006, available online at www.alcalc.oupjournals.org

Bustamante-Marin, X. M. and Ostrowski, L. E. 2017. Cilia and mucociliary clearance. Cold Spring Harbor Perspect. Biol. 9:a028241. doi: 10.1101/cshperspect.a028241

Chan KM, Delfert D, Junger KD. A direct colorimetric assay for Ca2+ -stimulated ATPase activity. Anal Biochem. 1986;157:375-80.

Chaurasia, S., Karwariya, A, Dev G. A. 2013. Effect of cement industry pollution on chlorophyll content of some crops at Kodinar, Gujarat, India. Proceedings of the International Academy of Ecology and Environmental Sciences 3: 288-295.

Cooper, Z., Bringolf, R. Cooper, R., Loftis, K., Bryan, A. L., Martin, J. A. 2017. Heavy metal bioaccumulation in two passerines with differing migration strategies. Sci. Total Environ. 592, 25–32. DOI: 10.1016/j.scitotenv.2017.03.055

Davenport, H. W., 1962. Effect of ouabain on acid secretion and electrolyte content of frog gastric mucosa. Proc Soc Exp Biol Med. 110(3): 613–615.

Egbe E. R., Nsonwu-Anyanwu A. C., Offor S. J., Usoro C. A. O., Etukudo M. H., Egbe, D. I. 2016. Cement Dust Exposure and Perturbations in Some Elements and Lung and Liver Functions of Cement Factory Workers. J Toxicol. 2016; 2016: 6104719. doi: 10.1155/2016/6104719

Ellman GL, 1959. Tissue sulfhydryl groups. Arch Biochem Biophys; 82: 70-7

Fatima, S. K., Prabhavathi, P. A., Padmavathi, P., Reddy, P. 2001. Analysis of chromosomal aberrations in men occupationally exposed to cement dust. Mutation Research 490 (2001) 179–186

Fell, K. M. A., Sikkeland, L. I. B., Svendson M. V., Kongerad, J. 2010. Airway inflammations in cement production workers. Occupational and environmental medicine. 67: 395-400

Gerbino, A., Debellis, L., Caroppo, R., Curci, S., Colella M., 2010. Cadmium inhibits acid secretion in stimulated frog gastric mucosa. Toxicol Appl Pharmacol. 2010 Jun 1;245(2):264-71.doi: 10.1016/j.taap.2010.03.010.

Ghosh, M.N. and Schild, H.O. (1958). Continuous recording of acid gastric secretion in the rat. Brit. J. Pharm. 13: 54-61.

Goth L, 1992. A simple method for determation of serum catyalase activation and revision of reference range. Clin Chim Acta; 196:143-52

Haber MM and Lopez I, 1999. Gastric histologic findings in patients with nonsteriodal anti-inflammatory drug associated gastric ulcer. Mod. Pathol.212, 592.

Horn-Ross PL, Ljung BM, Morrow M., 1997. Environmental factors and the risk of salivary gland cancer. Epidemiology. 8:414–9. doi: 10.1097/00001648-199707000-00011

Ilar J, 1996. Guide for the Care and Use of Laboratory Animals. 8th Edition.

Jumat, M., I., Hayati, F Rahim, Syed S., S., A., Saupin, S., Lukman, K., A., Jeffree, M. S., Benedict H., Lasimbang, F., K. 2021. Occupational lung disease: A narrative review of lung conditions from the workplace. Annals of Medicine and Surgery 64 (2021) 102245. https://doi.org/10.1016/j.amsu.2021.102245

Kakooei, H., Gholami, A., Ghasemkhani, M., Hosseini, M., Panahi, D., Pouryaghoub, G. 2011. Dust Exposure and Respiratory Health Effects in Cement Production. Acta Medica Iranica,; 50(2): 122-126.

Kang, Y., Pan, W., Liang, S., Li, N., Zeng, L., Zhang, Q., Luo, J., 2016. Assessment of relative bioavailability of heavy metals in soil using in vivo mouse model and its implication for risk assessment compared with bioaccessibility using in vitro assay. Environ Geochem Health. DOI 10.1007/s10653-015-9782-0

Kim, T., Cho, H. B., Kim, W. J., Lee, C. H., Chae, K. J., Choi, S-H, Lee, K. E., Bak, S. H., Kwon, S. O., Jin, G. Y., Choi, J., Park, E-K, Lin, C-L., Hoffman E. A., Choi, S. 2020. Quantitative CT-based structural alterations of segmental airways in cement dust exposed subjects. Respiratory Research 21:133. https://doi.org/10.1186/s12931-020-01399-9

Li, X., Brejnrod, A. D., Ernst, M., Rykær, M., Herschend, J., Olsen, N. M. C., Dorrestein, P. C., Rensing, C., Sørensen S. J., 2019. Heavy metal exposure causes changes in the metabolic health-associated gut microbiome and metabolites. Environment International. Volume 126, Pages 454-467

Liang, F., and Yan, B. 2020. Oxidative damage in the liver and kidney induced by dermal exposure to diisononyl phthalate in Balb/c mice. Toxicology and Industrial Health, Vol. 36(1) 30–40

Lowry OH, Rosenbrough NJ, Farr AL and Randall RJ, 1951 Protein measurement with Folin phenol reagent. J. Biol. Chem., 193, 265-275.

Madrid, J. A., Salido, G. M., Manas, E., Martinez M., Victoria D. E., Mataix F. J. 1983. Use of a bidirectional cannula to study biliary secretion in conscious dogs. Laboratory Animals (1983) 17, 307-310

Manjula R., Praveena, R., Clevin, R. R., Ghattargi, C. H., Dorle, A. S. Lalitha, D. H. 2013. Effects of occupational dust exposure on the health status of portland cement factory workers. International Journal of Medicine and Public Health. Vol 3, Issue 3.

Mbelambela, E. P., Eitoku, M., Muchanga, S. M. J., Villanueva, A. F., Hirota, R., Pulphus, T. Y., Sokolo, Yasumitsu-Lovell, G. J. K. Komori K., Suganuma N. 2018. Prevalence of chronic obstructive pulmonary disease (COPD) among Congolese cement workers exposed to cement dust, in Kongo Central Province. Environmental Science and Pollution Research. https://doi.org/10.1007/s11356-018-3401-4

Melin, A. M., Perromat, A., Deleris, G. 2000. Pharmacologic aplication of Fourier transform IR Spectroscopy: in vivo toxicity of carcon tetrachloride on rat liver. Biopolymers 57(3):160–168

Mohamed F. A. Elagib, I. A. Ghandour, Manar E. Abdel Rahman, Sara M. H. Baldo and Abubakr M. Idris, 2020. Influence of cement dust exposure on periodontal health of occupational workers. Toxin Reviews Volume 40, Issue 4 .https://doi.org/10.1080/15569543.2020.1737824

Nwafor PC, Odukanmi OA, Salami AT, Owonikoko M, and Olaleye SB, 2019. Evaluation of a Cement Dust Generation and Exposure Chamber for Rodents: Blood Heavy Metal Status, Haematological Variables and Gastrointestinal Motility in Rats Afr. J. Biomed. Res. Vol. 22 (January, 2019); 79- 87

Ogunbileje, J. O., and Akinosun, O. M. 2011. Biochemical and Hematological Profile in Nigerian Cement Factory Workers. Research Journal of Environmental Toxicology.

Ogunbileje, J. O., Sadagoparamanujam, V. M., Anetor, J. I., Farombi, E. O., Akinosun, O. M., Okorodudu, A. O. 2013. Lead, mercury, cadmium, chromium, nickel, copper, zinc, calcium, iron, manganese and chromium (VI) levels in Nigeria and United States of America cement dust. Chemosphere; 90; 2743–2749

Ohshima H, Bartsch H, 1994. Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis Mutat Res.;305(2):253-64. doi: 10.1016/0027-5107(94)90245-3.

Okonkwo, C. O. J., Ugwu, C. E., Anakor, A. C. F., Dike, C. C., Nwobodo, E. 2015. The Effects of Cement Dust on Haematological Parameters of Cement Workers in Asaba, Delta State, Nigeria. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) e-ISSN: 2319-2402,p- ISSN: 2319-2399. Volume 9, Issue 1 Ver. III, PP 05-08

Okonkwo, C. O. J., Ugwu, C. E., Anakor, A. C. F., Dike, C. C., Nwobodo, E. 2015. The Effects of Cement Dust on Haematological Parameters of Cement Workers in Asaba, Delta State, Nigeria. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) e-ISSN: 2319-2402,p- ISSN: 2319-2399. Volume 9, Issue 1 Ver. III, PP 05-08

Olaleye, S. B., Adaramoye, O. A., Erigbali, P. P., Adeniyi, O. S. 2007. Lead exposure increases oxidative stress in the gastric mucosa of HCl/ethanol-exposed rats. World J Gastroenterol 2007; 13(38): 5121-5126 [PMID: 17876879 DOI: 10.3748/wjg.v13.i38.5121]

Olatunbosun, N. O., Sawa, B. A., Jibrin, A., Ilori A. E. 2020. Assessment of effect of cement dust from cement factory on elemental properties of some cultivated crops, Obajana, Kogi State, Nigeria. Journal of Geography. Environment and Earth Science International 24(1): 63-69.

Owonikoko W. M., Emikpe B. O. and Olaleye S. B., 2021. Standardized experimental model for cement dust exposure, its attendant tissue heavy metal bioaccumulation and pulmonary pathological changes in rats. Toxicology report. Vol 8: 1169-1178

Oyanagui Y, 1984. Re-evaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal. Biochem. 1984, 142, 290–296.

Prakash, M., Shetty, M. S., Tilak, P., Anwar. N. 2009. Total thiols: Biomedical importance and their alteration in various disorder. Online J. Health Allied Sci., Vol. 8, No. 2.

Rachiotis, G., Kostikas, K., Pinotsi, D., Hadjikristodoulou C., Drivas, S. 2018. Prevalence of Lung Function impairment amond Greek cement production workers: a cross-sectional study. Industrial Health, vol 56, 49-52.

Ray, T. 2013. The parietal cell gastric H, K-ATPase also functions as the Na, K-ATPase and Ca-ATPase in altered states [version 2; peer review: 2 approved, 1 not approved] F1000Research, 2:165 (https://doi.org/10.12688/f1000research.2-165.v2)

Rezin, G. T., Scaini, G., Goncalves, C. L. Ferreira, G. K., Cardoso, M. R., Ferreira, A. G. K., Cunha, M. J., Schmitz, F., Varela, R. B., Quevedo, J., Wyse, A. T. S., Streck, E. L. 2014. Evaluation of Na+, K+-ATPase activity in the brain of young rats after acute administration of fenproporex. Revista Brasileira de Psiquiatria. 2014;36:138–142. doi:10.1590/1516-4446-2012-0956

Ribbons KA, Zhang X, Thompson JH, Greenberg SS, Moore WM, Kornmeier CM, Currie MG., Lerche N, Blanchard J, Clark DA and Miller MJS, 1995. Potential roles of Nitric Oxide in a model of chronic colitis in rhesus macaques. Gastroenterology; 108: 705-711

Romero AC, Ibuki FK, Nogueira FN. Sialic acid reduction in the saliva of streptozotocin induced diabetic rats. Arch Oral Biol. 2012; 57(9):1189–93. Epub 2012/03/17. doi: 10.1016/j.archoralbio.2012.02.016. PMID: 22421632.

Seçin, I.,Uijen, Maike J. M., Driessen, C. M. L. Herpen, C. M. L. v, Scheepers P. T. J., 2021. Case Report: Two Cases of Salivary Duct Carcinoma in Workers With a History of Chromate Exposure Front. Med., Sec. Pathology. https://doi.org/10.3389/fmed.2021.730403

Sheta, A. A. E. and Zahran, N. M. 2015. A Study of the Effect of Iron and Silicon as Cement Components on Cerebral and Cerebellar cortices of Albino Rats and the Possible Protective Role of Omega 3: A Toxicological and Histological Study Ain Shams Journal of Forensic Medicine and Clinical Toxicology. 24: 64-80

Szymonik-Lesiuk , S., Czechowska, G., Stryjecka-Zimmer, M., Słomka, M., Madro, A., Celiński, K., Wielosz, M. 2003. Catalase, superoxide dismutase, and glutathione peroxidase activities in various rat tissues after carbon tetrachloride intoxication. J Hepatobiliary Pancreat Surg 2003;10(4):309-15. doi: 10.1007/s00534-002-0824-5.

Tajudeen, Y., Okpuzor J., Fausat A. T. 2011. Investigation of General Effects of Cement Dust to Clear the Controversy Surrounding its Toxicity. Asian Journal of Scientific Research, 4: 315-325.

Terrón-Camero LC, Peláez-Vico MÁ, Del-Val C, Sandalio LM, Romero-Puertas MC, 2019. Role of nitric oxide in plant responses to heavy metal stress: exogenous application versus endogenous production. Journal of Experimental Botany, Volume 70, (17), 4477–4488, https://doi.org/10.1093/jxb/erz184

Ugbaja RN, Enilolobo MA, James AS, Akinhanmi TF, Akamo AJ, Babayemi DO and Ademuyiwa O, 2020. Bioaccumulation of heavy metals, lipid profiles, and antioxidant status of snails (Achatina achatina) around cement factory vicinities. Toxicology and Industrial Health 2020, Vol. 36(11) 863–875

Varshney R and Kale RK (1990) Effect of calmodulin antagonists on radiation induced lipid peroxidation in microsomes Int. J. Biol 158, 733-741.

Wei L, Zhang J, Wang C, Liao W, 2020. Recent progress in the knowledge on the alleviating effect of nitric oxide on heavy metal stress in plants. Plant Physiol Biochem ;147:161-171. doi: 10.1016/j.plaphy.2019.12.021.

Weupe, M., Lever, J. E. P., Kennemur, J. P., Bono, T. R., Phillips, S. E., Shei, R., Rowe, S. M., 2019. Moving mucus matters for lung health. Health. doi: 10.3389/frym.2019.00106

Wright, W. E., Bernstein, L., Peters, J. M., Garabrant, D. H., Mack, T. M. 1988. Adenocarcinoma of the stomach and exposure to occupational dust. Am J Epidemiol.;128:64–73.

Wright, W. E., Bernstein, L., Peters, J. M., Garabrant, D. H., Mack, T. M. 1988. Adenocarcinoma of the stomach and exposure to occupational dust. Am J Epidemiol.;128:64–73.

Wu J, Zhang Y, Hao R, Cao Y, Shan X, Jing, Y, 2019. Nitric Oxide Enhances Cytotoxicity of Lead by Modulating the Generation of Reactive Oxygen Species and Is Involved in the Regulation of Pb2+ and Ca2+ Fluxes in Tobacco BY-2 Cells. Plants, 8, 403; doi:10.3390/plants8100403

Yahaya, T., Oladele, E., Salisu, T., Orji, E., Zakari, Z., Liman, U. U., Gomo, C. B., Abdullahi, M. 2022. Toxic metals in cement induced hematological and DNA damage as well as carcinogenesis in occupationally-Exposed block-factory workers in Lagos, Nigeria, Egyptian Journal of Basic and Applied Sciences, 9:1, 499-509, DOI: 10.108

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