Genetic Diversity of Plasmodium falciparum Based on Merozoite Surface Proteins 1 and 2 Genes
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genetic diversity
merozoite surface proteins 1 and 2
Plasmodium falciparum

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Nyamngee , A. (2022). Genetic Diversity of Plasmodium falciparum Based on Merozoite Surface Proteins 1 and 2 Genes. African Journal of Biomedical Research, 25(2), 163–167.


Genetic diversity of Plasmodium falciparum is an important feature that makes the parasite a successful pathogen and is a risk factor for generating mutant variants involved in pathogenicity, drug resistance and immune evasion. A study was designed to determine the diversity of P. falciparum isolates based on merozoite surface protein 1 and 2, determine the predominant circulating allelic families and multiplicity of infection  in Nigeria. The diagnosis was based on finding the characteristic asexual stage of the parasite in Giemsa-stained blood smears under a compound microscope. The Deoxyribonucleic acid was extracted from P. falciparum positive blood using Chelex extraction method followed by PCR-genotyping, targeting the merozoite surface proteins. Nested polymerase chain reaction and  restriction fragment length polymorphisms were used to detect Plasmodium falciparum chloroquine resistance transport, P. falciparum multidrug resistance 1, P. falciparum dihydrofolate reductase and P. falciparum dihydropteroate synthase. Data were analysed using the Statistical Packages for Social Sciences Version 21.0 at a significance level of P<0.05. Overall, multiplicity of infection with MSP 1 and MSP 2 markers was 1.32 and 1.24 respectively. P. falciparum isolates demonstrated diverse nature in respect of MSP 1 (block 2) and MSP 2 (block 3). All the families of MSP 1 and MSP2 were detected. It was concluded that the genetic diversity of P. falciparum was comparatively high. Therefore, strategies that reduce multiple-strain infections should be implemented in order to improve antimalarial drug efficacy and reduce the rate of spread of drug resistance.
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Al-abd NM, Mahdy MA, Al-Mekhlafi AM, Snounou G, Abdul-Majid NB and Al-Mekhlafi HM. The Suitability of P. falciparum Merozoite Surface Proteins 1 and 2 as Genetic Markers for in-Vivo Drug Trials in Yemen. PLoS ONE 2013; 8 (7):e67853.doi: 10.1371/journal. Pone. 0067853.

Amodu OK, Gbadegesin RA, Ralph SA, Adeyemo AA, Brenchley PE and Ayoola OO. Plasmodium falciparum Malaria in South-West Nigerian Children: is the Polymorphism of ICAM-1 and E-Selectin Genes Contributing to Clinical Severity of Malaria? Acta Tropica 2005; 95:248-255.

Balam S, Olugbile S, Servis C, Diakite M, D’Alessandro A, Frank G et al. Plasmodium falciparum Merozoite Surface Protein 2: Epitope Mapping and Fine Specificity of Human Antibody Response Against Non-Polymorphic Domains. Malaria Journal 2014; 13:510

Cheesbrough M. Parasitological Tests: Examination of Blood for Malaria Parasites in District Laboratory Practice in Tropical Countries Part 1, Second Edition (Updated) Cambridge University Press New York, 2000; 239-258.

Christopher GA, Christopher AM, Linda R, Kaye W, Michelle JB, Vivian K et al. Antigenic Charactarization of an Intrisically Unstructured Protein, Plasmodium falciparum Merozoite Surface Protein 2. Journal of Infection and Immunity 2012;8(12):4177

Deborah S, Hosea KM, Mugasa JP and Salim A. Genetic Diversity of Plasmodium falciparum Strains in Children Under Five Years of Age in Southeastern Tanzania. The Open Tropical Medicine Journal 2010; 3: 10-14.

Dinesh K, Sunil D, Bipul R, Diganta G, Manab D, Lokendra S et al. Genetic Polymorphism and Amino Acid Sequence Variation in Plasmodium falciparum Glurp R2 Repeat Region in Assam, India, At an Interval of Five Years. Malaria Journal 2014; 13:450

Ferreira MU, Ribeiro WL, Tonon AP, Kawamoto F and Rich SM. Sequence Diversity and Evolution of The Malaria Vaccine Candidate Merozoite Surface Protein-1 (MSP-1) of Plasmodium falciparum. Genetic 2003; 75: 30465-30475.

Hongying J, Na L, Vivek G, Martine MZ, Sudhir V, Vijayaraj N et al. High Recombination Rates and Hotspots in a Plasmodium falciparum Genetic Cross. Genome Biology 2011;12:33

Hussain MM, Sohail M, Kumar R, Branch OH, Adak T and Raziuddin M. Genetic Diversity in Merozoites Surface Protein 1 And 2 Among P. falciparum Isolates From Malarious Districts of Tribal Dominant State of Jharkhand, India. Annals of Tropical Medicine and Parasitology 2011; 105(8): 579-592.

Lilian R, Cesare B, Paulo R, Daina P, Luciene A, Evelyn K et al. Antibodies Against the Plasmodium falciparum. Memorias Institute Oswaldo Cruz. 2011; 106(1):34-43

Muzami MH, Mohammed SB and Ibrahim MH. Genetic Diversity of Plasmodium falciparum Field Isolates in Central Sudan interfered by PCR Genotyping of Merozoite Surface Protein 1and 2. North American Journal of Medical Sciences 2013;5(2):95-101

Olashehinde GI, Yah CS, Singh R, Ojurongbe OO, Ajayi AA , Valecha N. genetic diversity of plasmodium falciparum field isolates from south western Nigeria. African health sciences 2012; 12 (3): 355-361.

Ojurongbe O, Fagbenro-Beyioku AF, Adeyeba OA, Kun JF. Allelic Diversity of Merozoite Surface Protein 2 Gene of Plasmodium falciparum Among Children in Osogbo, Nigeria . West Indian Medical Journal 2011; 60 (1)

Omina ME and Amany ME. Limited Genetic Diversity Among Plasmodium falciparum Isolates Using Nested PCR in Jazan Area, Saudi Arabia. Asian Pacific Journal of Tropical Biomedicine 2015; 5(5): 407-411.

Oyebola KM, Emmanuel TI, Olukosi YA, Bamidele AI, Chimere OA, Ajibaye AA et al. Genetic Diversity and Complexity of Plasmodium falciparum Infections in Lagos, Nigeria. Asian Pacific Journal of Tropical Biomedical Science 2014; 4 (1): 87-91

Oyedeji SI, Awobode HO and Jurgen K. Limited Genetic Diversity and Low Multiplicity of P. falciparum Infections in Children With Severe Malaria in Lafia, North-Central Nigeria. Journal of Experimental And Clinical Medicine 2013; 5 (4): 143-147.

Panita G, Charlotte AL, Stuart DT, Youry S, Chanthap L, Michelle S et al. Evaluation of Parasite Subpopulations and Genetic Diversity of the MSP-1, MSP-2 and Glurp Genes During and Following Artesunate Monotherapy Treatment of Plasmodium falciparum Malaria in Western Cambodia. Malaria Journal 2013; 12:403

Raj DK, Das BR, Dash AP and Supakar PC. Genetic Diversity in The MSP-1 Gene of Plasmodium falciparum in Different Malaria Endemic Localities. The American Journal of Tropical Medicine and Hygiene 2004; 71: 285-289.

Rich SM and Ayala FJ. Population Structure and Recent Evolution of Plasmodium falciparum. Proceedings of the National Academy of Sciences of The United States of America 2000;97: 6994-7001.

Sedigheh Z, Akram AM, Samaneh Z and Navid DD. Non-variant specific Antibody Response to the C-terminal Region of MSP-1 of Plasmodium falciparum (PfMSP-119) in Iranians Exposed to Unstable Malaria Transmission. Malaria journal 2010; 9 (1):257

Stubbs J, Olugbile S, Saidou B, Simpore J, Corradin G and Lanzavecchia A. Strain Transcending Fc Dependent Killing of Plasmodium falciparum by Merozoite Surface Protein 2 Allelic Specific Human Antibodies. Journal of Infectious Immunology 2011;79: 1143-1152

Snounou G, Zhu X, Spiripoon N, Jara W, Thaithong S, Brown K et al. Biased Distribution of Merozoite Surface Protein 1 and Merozoite Surface Protein 2 Allelic Variant in Plasmodium falciparum Populations In Thailand. Transactions of Royal Society of Tropical Medicine and Hygiene 1999; 93: 369-374.

Wanji S, Arnaud JK, Ebanga EJ, Helen KK, Nicholas T, Judith LN et al. Genetic Diversity of Plasmodium falciparum Merozoite Surface Proteins-1 Block 2 in Sites of Contrasting Altitudes and Malaria Endemicities in The Mount Cameroon Region. American Journal of Tropical Medicine and Hygiene 2012; 86 (5): 764-774.

Wright PE and Dyson JH. Intrinsically Unstructured Proteins: Re-assessing the Protein Structure-Function Paradigm. Journal of Molecular Biology 1999; 293: 321-331

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