Effects of Petrol Exposure on Glucose, Liver and Muscle glycogen levels in the Common African toad Bufo regularis
Palavras-chave:
Petrol, Blood glucose, Liver glycogen, Muscle glycogenResumo
Summary: This study investigated the effects of exposure to petrol on blood glucose, liver and muscle glycogen levels in the common African toad Bufo regularis. A total of 126 adult toads of either sex weighing between 70-100g were used for this study. The experiment was divided into three phases. The phase 1 experiment the acute toxicity test consisted of animals divided into six groups of 10 toads per group and were exposed to water (H2O), H2O + Tween 80, 2ml/l, 3ml/l, 5ml/l, and 10ml/l of petrol respectively for 96 hours using the static renewal bioassay system. In the Phase 2 experiment, the animals were exposed to H2O, H2O + Tween 80, 0.14ml/l, 0.3ml/l, 0.6ml/l, and 1.13ml/l of petrol respectively for 3 days; while in phase 3 experiment they were exposed to petrol solutions for 14 days. After the various exposures, the blood glucose, liver and muscle glycogen contents were determined using standard methods. The results of the study showed that the median lethal concentration of petrol (96 hours LC50) was 4.5ml/l and sub-lethal concentration of petrol caused mortality of animals. Exposure to petrol solutions for 3 days had no significant effect on blood glucose level of the animals but caused significant decrease in the liver and muscle glycogen levels at high concentrations. In the animals exposed to petrol solutions for 14 days, there was a significant increase in glucose levels and significant reduction in liver and muscle glycogen levels at high concentrations when compared with the control. The results show that sub-lethal concentrations of petrol can cause mortality of animals, hyperglycemia and reduction in liver and muscle glycogen levels. The effects of petrol exposure on carbohydrate metabolism depend on the concentration and duration of exposure.
Referências
Abdel-Tawwab, M. (2012). Chronic effect after acute exposure to commercial petroleum fuels on physiological status of Nile tilapia, Oreochromis niloticus (L.) International Aqua. Res. 4:11.
Adebrium, A. (1976) Biochemical toxicology of environmental agents. North Holland biomedical press, Elsevier pp 210.
Alford, R.A. and Richards, S. J. (1999). Global amphibian declines: a problem in applied ecology. Annual. Rev.Ecol. Systematics 30 (1): 133-165.
Almeida, J. A., Novelli, E. L. B., Dal-Pai Silva, M., Alves- Junior, R. (2001). Environmental cadmium exposure and metabolic responses of the Nile tilapia Oreochromis niloticus. Environ. Pollut. 114: 169-175.
Alonso-Alvarez, C. Perez, C. and Velanddo, A. (2007). Effects of acute exposure to heavy fuel oil from the prestige spill on a seabird. Aqua. Toxicol. 84:103-110.
Andersen, Z.J. Raaschou-Nielsen, O. Ketzel, M. Jensen, S.S. Hvidberg, M. Loft, S.Tionneland, A.O. (2012). Diabetes incidence and long-term exposure to air pollution: a cohort study. Diabetes Care 35: 92-98.
Anigbogu, C.N. Idowu, A.J. Anunobi, C.C. (2011). Petroleum contamination altered neuromuscular function and structural integrity in the sciatic nerve gastrocnemius muscle preparation of toad. International. J. Toxicol. Pharmacol. Res. 3(3):1-8.
Barton, B.A. (2002). Stress in Fishes: A divert of responses with particular reference to changes in circulating corticosteroids. Integ. Comp. Biol. 42: 517-525.
Blaustein, A.R. and Wake, D.B. (1995). The puzzle of declining amphibian populations. Scientific American. 272(4):56-61.
Blaustein, A.R. Romansic, J.M. Kiesecker, J.M.Hatch, A.C. (2003).Ultraviolet radiation, toxic chemicals and amphibian population declines. Diversity and Distribution 9 (2):123-140.
Brook, R.D. Jerrett, M. Brook, J.R. Bard, R.L. Finkelstein, M.M. (2008). The relationship between diabetes mellitus and traffic-related air pollution. J. Occup. Environ. Med. 50: 32-38.
Cairney, S. Maruff, P. Burns, C. B. Currie, J. Currie, B. J. (2005). Neurological and cognitive recovery following abstinence from petrol sniffing. Neuropsychopharmacol.30: 1019-27.
Dede, E.B. Kaglo, H.D. (2001). Aquatoxicological effects of Water Soluble Fractions (WSF) of diesel fuel on O. niloticus fingerlings. J. Appl. Sci.Environ. Management, 5(1): 93 – 96.
Ezike, C., Ufodike, E.B.C. (2008). Plasma Glucose and Liver Glycogen of African Catfish (Clarias gariepinus). J. Fish.Intern. 3(2): 46-48.
Fafioye, O.O. (2006). Effect of chronic exposure to water soluble fractions of Forcados crude oil on the growth and development of African catfish Clarias gariepinus larvae. Global J. Pure. Appl.Sci., 13(2): 179- 182.
Farrar, E.S. and Frye, B.E. (1979). Factors affecting normal carbohydrate levels in rana pipiens. Gen. Comp. Endocrinol. 39:358-371.
George, K. R., Malini, N. A., Sandhya Rani, G. O. (2012). Biochemical changes in liver and muscle of the cichlid, Oreochromis mossambicus (Peters, 1852) exposed to sub-lethal concentration ofmercuric chloride. Indian. J. Fish., 59(2): 147-152.
Golet, G.H. Seiser, P.E. McGuire, A.D. Roby, D.D. Fischer, J.B. Kuletz, K.J. Irons, D.B. Dean, T.A. Jewett, S.C. Newman, S.H. (2002). Long-term direct and indirect effects of the Exton Valdez oil spill on pigeon guillemots in Prince Willam Sound, Alaska. Mar. Ecol. prog. Ser. 241: 287-304.
Isehunwa. O. G. and Alada, A.R.A. (2016) Seasonal and sex variation in carbohydrate levels of the Common African toad bufo regularis, Cand J Pure & Appl. Sci. 10(2): 3847.
Jermyn, M. A. (1975). Determination of Glycogen. Increasing the sensitivity of the anthrone method for carbohydrate. Analytical Biochem. 68: 322-335.
Krämer, U., Herder, C., Sugiri, D., Strassburger, K., Schikowski, T. et al. (2010). Traffic-related air pollution and incident type 2 diabetes: results from the SALIA cohort study. Environ Health Perspect 118: 1273-1279.
Levesque, H.M., Moon, T.W., Campbell, P.G., Hontela, A. (2002). Seasonal variations in carbohydrate and lipid metabolism of yellow perch (Perca flavescens) chemically exposed to metals in the field. Aqua. Toxicol. 60 (3-4): 257-267.
Litovitz, T. (1988). Mycardial sensitization following inhalation abuse of hydrocarbons. Occup. Med.3, 567-568.
Moles, A. and Norcoss, B.L. (1998). Effects of oil-laden sediments on growth health of juvenile flatfishes. Canad. Fish. Aqua. Sci, 55, 605-610.
Moszczynski, P. Lisiewicz, J. (1978). Enzymes of neutrophils in workers occupationally exposed tobenzene, toluene and xylene, Acta Histochem. 61(1)1-19.
Nakano, T. and Stimpson, N. (1967). Catecholamines and carbohydrate concentrations in rainbow trout, Salmo gairdneri in relation to physiological disturbance. J. Fish Biol. 2: 1901-1915.
Newman, S.H. Anderson, D.W. ZiCardi, M.H. Trupkiewicz, J.G. Tseng, F.S. Christopher, M.M Zinkl, J.G. (2000) An experimental soft-release of oil-spill rehabilitated American coots (fulica Americana):11. Effects on health and blood parammeters. Environ.pollut. 107: 295-304.
Oladimeji, A. A. and Ologunmeta, R.T. (1987). Toxicology of water borne lead to Tilapia niloticus. Nigerian J. Aqua. Sci.2: 19-24.
Oladimeji, A.A., Onwumere, B.G. (1988). Sub-lethal effects of treated effluents from NNPC refinery Kaduna to Oreochromis niloticus (Tilapia). The PetroleumIndustry and the Nigerian Environment, Proceedings of the Nigeria National Petroleum Corporation (NNPC), pp. 256 – 262.
Omoregie E., Ufodike E.B.C. (1999). Effects of crude oil exposure on growth, feed utilization, and food reserves of the Nile tilapia, Oreochromis niloticus Trewavas. Acta Hydrobiol., 41, 3/4, 259-268.
Omoregie, E. Ufodike, E.B.C. and Onwuliri, C.O.E. (1995). Effects of petroleum effluent on carbohydrate reserves of Nile tilapia, Oreochromis niloticus (L), West Afri. J. Biol. Sci. 3:70-76.
Ozdikicioglu F., Dere E. (2004). Effects of benzene on glycogen levels of liver and muscle tissues and on blood glucose of rats.Acta Veterinaria (Beograd). Vol. 54, 379-394.
Pearson, J.F., Bachireddy, C., Shyamprasad, S., Goldfine, A.B., Brownstein, J.S. 2010. Association between fine particulate matter and diabetes prevalence in the U.S. Diabetes Care 33: 2196-2201.
Renner, K.O.Don-pedro, K.N. and Nubi, O.A. (2008). Oil spillage and its impact on the edible mangrove periwinkle, Tympanotonus fuscatus Var Radula (L). Sci. World J. 3:13-16.
Schneider, M. S., Mani, M. M., Masters, F. W. (1991). Gasoline-induced contact burns. J. Burn. Care. Rehabil 12, 140-3.
Seifter, S., Dayton, S., Novic, B., Muntwyler, E. (1950). Arch. Biochem. 25, 191.
Simonato, J. D., Fernandes, M. N., Martinez, C. B. R. (2013). Physiological effects of gasoline on the freshwater fish Prochilodus lineatus (Characiformes: Prochilodontidae). Neotrop. ichthyol. vol.11 no.3
Sobha, K., Poornima, A., Harini, P., Veeraiah, K. (2007). A study on the biochemical changes in freshwater fish Catla catla exposed to the heavy metal toxicant, cadmium chloride. Kathmandu University Journal of Science, Engineering and Technology. 1: 4.
Soman, G., Philip, G. (1974). Aromatic compounds as allosteric inhibitors of glycogen phosphorylase b, Bioch Biophys Acta (BBA), Enzymology, 358, 354-62.
Spraque, J.B. (1975). Measurement of pollution toxicity to fish. In Bioassay methods for acute toxicity. Water Resources, 3: 346-349.
Trinder, E. (1969). Determination of blood glucose using 4 amino phenazone as oxygen acceptor. J. Chem. Pathol.22 (1969) 246.
Ujowundu, C.O., Kalu, F.N., Nwaoguikpe, R.N Kalu, O.I., Ihejirika, C.E., Nwosunjoku, E.C., Okechukwu, R.I. (2011). Biochemical and physical characterization of diesel oil contaminated soil in southeastern Nigeria. Res. J. Chem. Sci., 1(8), 57-62.
Vinodhini, R.. Narayanan, M. (2009). The impact of toxic heavy metals on the haematological parameters in common carp (Cyprinus carpio L.). Iran J. Environ. Health. Sci. Eng., Vol. 6, No. 1, pp. 23-28.
Vinodhini, R. and Narayar, M. (2009). Heavy metal induced histopathological alterations in selected organs of cypinus caropio L. (common carp). International J. Environ. Res. 3 (1): 95-100.
Wedemeyer, C.P. Mcleary, D.J. Goodyear, C.P. (1984). Assessing the tolearance of fish and fish population to environmental stress: Problems and methods of monitoring. In: Cairn V.W. Hodson P.V and Nriage J.O.(eds) Contamination effects on fisheries. New York. John Wiley and Sons Inc. 164-193.
Zikic, R.V.,Stajn, A.S. Ognjanovic, B.I., Pavlovic, S.Z., Saicic, Z.S. (1997). Activities of superoxide dismutase and catalase in erythrocytes and transaminases in the plasma carps (Cyprinus carpio. L.) exposed to cadmium. Physiol. Res, 46: 391-396.
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