High Reticulocyte Count with Abnormal Red Blood Cell Morphology in Normal Wistar Rats after Garlic Administration
Gako-hitzak:
Garlic, Red blood cells, Reticulocytes, RatLaburpena
Summary: Despite the high acceptability of Allium sativa (Garlic) as a remedy for many diseases as earlier stated by many researchers, previous studies have shown that chronic and unregulated consumption of garlic may result to intra vascular haemolytic anaemia in rats. The present study was conducted to examine the effect of crude extract of garlic on microscopic status of red blood cells and some other haematological indices of normal albino rats. The animals were grouped into two; group 1 were normal animals treated with water while group 2 were normal animals administered 150mg/kg body weight of crude extract of garlic on alternate days for three weeks. At the end of three weeks treatment, blood samples obtained from the tail vein of the rats were used for haematological indices and erythrocyte morphology. The values obtained were expressed as Mean± SEM and compared using student t test. The results showed that there was no significant difference in the PCV which was 43.20 ± 0.80% and 45.00 ± 0.36% in both control and experimental groups respectively. However, the RBCs were significantly decreased (P< 0.05) from 166.80 ± 3.44 x106 μL-1 in the control group to 87.80 ± 9.34 x106 μL-1 in the treatment group. The percentage reticulocyte counts on the other hand significantly increased from 2.60±4.25% in control group to 11.20± 16.4% in treated group. Fragmented RBCs with a lot of schistocytes with adequate platelets were seen on peripheral blood film of crude garlic treated rats as compared to control. Our results suggested intravascular haemolysis and numerous reticulocytes on blood film confirmed our view on bone marrow response. The presence of schistocytes and acanthocytes may be an indication that the liver is involved in the observed effect.
Erreferentziak
Amagase, H., Petisch, B.L., and Masuura, H. (2001). Intake of garlic and its bioactive components. J.Nutrition, 131, pp.9555-9625.
Alexander, R.R., and Griffith, J.M. (1993). Haematocrite In: Basic Biochemical method, 2nd Ed. John Willey and Sons Inc. publication New York, pp 186-187.
Aluoch, J. R. (1995). The presence of Sickle Cells in the Peripheral Blood Film. Specificity and sensitivity of homozygous Sickle cell disease in kenya. Trop. Geogr. Med., 47(2), 89-91
Aka, L.O., Pilau, N.N., and Obidike, R.T., (2010). The effects of dietery supplementation of Allium sativium on some vital biochemical parameters in male albino rats. Sokoto journal of Veterinary Sciences, 8(1), pp.26-30.
Brune, T., Michel, H., Romain, M., Dominique, G., Anne, G., and Yves D. (2001). Histologic Features of the Liver in Insulin Resistance-Associated Iron Overload. Am J Clin Pathol, 116, pp.263-270.
Choi, J.W. and Pai S.H. (2001). Subpopulations and reticulocyte maturity index (RMI) rise as body iron status falls. Am J Hemato, 67, pp.130-5.
Dhaliwal, G., Cornett, P.A., and Tierney, L.M., (2004). Hemolytic Anemia. Am. Fam. Physician, 69, pp. 2599–2606.
Eidi, A., Eidi, M., and Fismaeili, E. (2006). Antidiabetic effect of garlic (Allium Sativum L.) in Normal and Streptozotocin –induced diabetic Rats. Phytomed, 13 (9-10), pp. 624-629.
Gurpreet, D., Patricia, A., and Lawrence, M. (2004). Haemolytic Aneamia. American Family Physician, 69(11), pp2600-2605.
Hussein, J.S., Oraby, F.S., and El-Shafey, N. (2007). Anti-hepatotoxic effect of garlic and onion oils on Ethanol-induced liver injury in Rats. Journal of applied Sciences Research, 3(11), pp.1527-153.
Jaiswal, N. and Rzvi, I.S. (2011). Protective effect of different layers of onion extracts (Allium Cepa L.) on markers of oxidative stress in erythrocytes. Cell Memb. Free Rad.Res, 3(2), pp. 171-177.
Kashinath, R.T. (1990). Hypolipidemic effects of disulphides in rats fed high lipid diet and/or ethanol. PhD thesis, University of Bangalore, India. Pp. 221-225.
Kumar, S. and Bandyopadhyay, U. (2005). Free hemetoxicity and its detoxification systems in human. Toxicol. Lett., 157, pp.175–188.
Luczynski, W., Ratomski K., Wysocka, J., Krawczuk-Rybak, M., Jankicwicz, J. (2006). Immature reticulocyte fraction (IRF): An universal marker of hemopoiesis in children with cancer. Advances in Medical sciences, 57, pp 188-190.
Meleola, D. and Edwin, X.C. (1979). Sulfhydryl compounds. In. Enzymology (3rd ed) Longman Pub. pp.220.
Nasr, A. Y. (2014). Protective effect of aged garlic extract against oxidative stress induced by cisplatin on blood cells parameters and hepatic antioxidant enzymes in rats. Toxicology reports, (1), pp.682-691.
Salami, H. A., John, A., and Ekanem A.U. (2012). The effect of aqueous preparation of Allium cepa (Onion) and Allium sativa (Garlic) on erythrocyte osmotic fragility in wister rats. In vitro and in vivo studies. Niger. J. physiol. Sci., 27, pp.029-034.
Singh. V.K. and Singh D.K. (2008). Pharmacological Effects of Garlic (Allium Sativum L.). Annu. Rev Biomed Sci, 10, pp.6-26.
Shankaran, M., Vadivel, A., and Thangam, A., (2010). Curative effect of Garlic on alcoholic liver diseased patient. Jordan Journal of Biological Sciences, 3(4), pp.147-152.
Shashikanth, K., Basappa, S., and Murthy, V. (1986). Effect of feeding raw and boiled garlic (Allium sativum L.) extracts on the growth, ceacal microflora and serum protein of albino rats. Nutrition and Reproduction International, 33, pp.313-319.
Umar, L.A., Kaldapa, J.T., Otukonyung, E., EIgbokwe, I.O, Omage J.J., Madu, S., and Buratai L.B (1996). Garlic induced Haematolytic anaemia in garlic fed rats may be associated with decrease levels of erythrocyte reduced glutathione and serum ascorbic acid. West African journal of biological Sciences.4, pp.73-81.
Yamamoto, D. and Meade Y. (1992). Susceptibility of onion-induced haemolysis in dogs with hereditary high erythrocyte reduced gluthatione and potassium concentration. Am.J.Vet. Res. 53,pp.134-137.
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