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Abstract
In this study, the effects of maternal exposure to Bonny Light Crude Oil (BLCO) on reproductive functions of the offspring was investigated in Wistar rats. Ten pregnant rats were divided into two groups (n=5). Group 1 served as the control, it was administered 0.75ml/Kg bwt/day normal saline and Group 2 was administered 0.75ml/Kg bwt/day BLCO. Serum hormonal profile, sperm indices, estrous cycle length and pubertal timing were assessed as measures of reproductive function. Tissue Malondialdehyde, Catalase and SOD activities were assessed as indices of oxidative stress. Results obtained showed that BLCO significantly (p<0.05) reduced birth weight, anogenital distance (AGD) at birth, sperm count, motility and normal morphology, serum testosterone, testicular and epididymal SOD and catalase activities in the male offsprings. However, days of preputial separation, relative weight of testis and epididymis, testicular and epididymal MDA were significantly (p<0.05) raised by gestational exposure to BLCO. In the female offspring, birth weight, AGD at birth, relative weight of ovaries and uterus, SOD, catalase activities, serum LH were significantly reduced by BLCO exposure during gestation. Moreover, uterine and testicular MDA, serum estradiol and FSH were significantly increased by BLCO treatment during gestation.
In conclusion, maternal exposure to BLCO during gestation may alter reproductive indices in the offspring and increased occurrence of oxidative stress in reproductive structures in male and female offspring of Wistar rats.
References
Aitken R.J. (1994). A free radical theory of male infertility. Reprod Fertil Dev 6: 19–23.
Aitken R.J and Krausz C. (2001). Oxidative stress, DNA damage and the Y chromosome.
Reproduction. 122: 497–506.
Aron D.C., Finding J.W. and Tyrrell J.B. (2007). Glucocorticoids and adrenal androgen;
Greenspan‟s Basic and Clinical Endocrinology (David and Shoback) 8th edition Mc
Graw Hill pp 246-295
De Boo, H.A, & Harding, J.E. (2006). The developmental origins of adult disease (Baker) hypothesis. Aust N Z J Obstet Gynaecol, 46(1), 4-14.
Federica Tramer, Francesca Rocco, Fulvio Micali, Gabriella Sandri, Enrico Panfili. (1998). Antioxidant systems in rat epididymal spermatozoa. Biology of reproduction 59 (4), 753-758, 1998.
Fischer CJ, Saha A, Cairns BR. (2007) Kinetic model for the ATP-dependent translocation of Saccharomyces cerevisiae RSC along double-stranded DNA. Biochemistry 46(43):12416-26
Giwa-Osagie O.O. (2003). Nigeria has twelve million infertile persons. Pharma News 25:48-49.
Hara P Misra, Irwin Fridovich. (1972). The purification and properties of superoxide dismutase from Neurospora crass. Journal of Biological Chemistry 247 (11), 3410-3414, 1972.
Hugo B Fischer, John E List, C Robert Koh, Jorg Imberger, Norman H Brooks. (2013). Mixing in inland and coastal waters. Elsevier, 2013
Jeje SO, Ola-Mudathir FK, Raji Y (2017). Experimental maternal treatment with dexamethasone during lactation induces neonatal testicular and epididymal oxidative stress; Implications for early postnatal exposure. Pathophysiology. 2017 Dec;24(4):261-265. doi: 10.1016/j.pathophys.2017.06.002. Epub 2017 Jul 4.
Jeje S.O and Raji Y (2017). Maternal treatment with dexamethasone during gestation alters sexual development markers in the f1 and f2 male offspring of Wistar rats. Journal of Developmental Origin of Health and Diseases. 8(1): 101-112. doi: https://doi.org/10.1017/S2040174416000453
John A Buege, Steven D Aust. (1978). Microsomal lipid peroxidation. Methods in enzymology 52, 302-310, 1978.
Joseph S. Ostby L. Earl Gray Jr. (2004). Transgenerational (In Utero/Lactational) Exposure to Investigate the Effects of Endocrine Disrupting Compounds (EDCs) in Rats. Current Protocols in ToxicologyVolume 19, Issue 1.
Kenichiro Urata, Hisashi Narahara, Yuichiro Tanaka, Toru Egashira, Fusako Takayama, Isao Miyakawa. (2001). Effect of endotoxin-induced reactive oxygen species on sperm motility. Fertility and sterility 76 (1), 163-166, 2001
Kumar S. (2008). Is environmental exposure associated with reproductive health impairments? J. Turk Ger Gynecol Assoc 9: 60-69.
Marcondes, F., Bianchi, F., & Tanno, A. (2002). Determination of the estrous phase of rats: some helpful considerations. Brazilian Journal of Biology, 62(4a),609-614.
Ola‐Mudathir KF, Suru SM, Fafunso MA, Obioha UE, Faremi TY. 2008. Protective roles of onion and garlic extracts on cadmium‐induced changes in sperm characteristics and testicular oxidative damage in rats. Food Chem Toxicol 46: 3604– 3611.
Oliver H Lowry, Nira J Rosebrough, A Lewis Farr, Rose J Randall. (1951). Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75. PMID: 14907713.
Omayone TP, Salami AT, Olopade JO, Olaleye SB. Attenuation of ischemia-reperfusion-induced gastric ulcer by low-dose vanadium in male Wistar rats. Life Sci. 2020 Oct 15;259:118272. doi: 10.1016/j.lfs.2020.118272. Epub 2020 Aug 12. PMID: 32800836.
Orisakwe O.E., Akumka D.D., Njan A.A., Afonne J.O. (2004). Testicular toxicity of Nigeria bonny light crude oil in male albino rats. Reprod Toxicol 18:439-442.
Oruambo I.F., Jones A.B. (2007). Alterations in the concentrations of liver mitochondrial DNA: Cytoplasmic total hydrocarbon and calcium in guinea pigs after treatment with Nigerian light crude oil. Int J Environ Res Public Health 4:23-27.
Raji Y, Bolarinwa AF. Antifertility activity of Quassia amara in male rats - in vivo study. Life Sci. 1997;61(11):1067-74. doi: 10.1016/s0024-3205(97)00615-2. PMID: 9307052.
Raji Y, Udoh U.S, Mewoyeka O.O, Ononye F.C, Bolarinwa A.F. (2003). Implication of reproductive endocrine malfunction in male antifertility efficacy of Azadirachta indica extract in rats. African journal of medicine and medical sciences 32 (2), 159-165, 2003.
Raji Y, Hart VO. Influence of prolonged exposure to Nigerian Bonny light crude oil on fertility indices in rats. Niger J Physiol Sci. 2012 Jun 7;27(1):55-63. PMID: 23235309.
Rivas A., Fisher J.S., Mckinnell, Atanassova N. and Sharpe (2002). Induction of
reproductive tract developmental abnormalities in the male rat by lowering androgen production or action in combination with a low dose of diethylstilbesterol: evidence for importanceof the androgen –estrogen balance. Endocrinology. 143: 4797-4808
Sharp R.M. (2001). Hormones and testis development and the possible effects of
environmental chemicals. Toxicol. Lett. 120: 221-232
Sherry H Stewart, Matt G Kushner. (2001). Introduction to the special issue on “Anxiety Sensitivity and Addictive Behaviors”. Addictive Behaviors 26 (6), 775-785, 2001
Shrestha, J., Shanbhag, T., Shenoy, S., Amuthan, S., Prabhu, K., Sharma, S., … Kafle, S. (2010). Antiovulatory and abortificient effects of Araca catechu (betel nut) in female rats. India J. Pharmacol(42), 306-311.
Tramer F., Rocco F., Micali F., Sandri G. and Panfili E.(1998). Antioxidant systems in rat epididymal spermatozoa. Biol Reprod. 59:753–758.
Tutian, Z., Fazelipur, S., & Shadkhast, M. (2008). Study of histologic and histometric change on ovarian cortex after administration of morphine in mice. Iranian Journal of Veterinary Medicine, 4(4), 90-6.
Welsh M., Saunders P.T., Fsiken M., Scott H.M., Hutchison G.R., Smith L.B. and Sharpe R.M. (2008). Identification in rats of aprogramming window for reproductive tract masculinisation, distruption of which leads to hypospadias and cryptochidism. J. Clin . Invest. 118: 1479-1490
Zubkova E.V. and Robaine.B. (2004). Effects of Glutathione Depletion on Antioxidant
Enzymes in the Epididymis, Seminal Vescicle and Liver and on Sperm motility in
Ageing Brown Norway Rats. Biology of Reproduction 71: 1002-1008
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