In Vitro and In Vivo Investigation of the Antidiabetic Mechanisms of Action of Rauvolfia vomitoria leaf

Authors

  • Peter Akpojotor University of Benin, Benin City, Nigeria
  • Maureen Isoken Ebomoyi University of Benin, Nigeria

DOI:

https://doi.org/10.54548/njps.v39i1.20

Keywords:

Rauvolfia vomitoria leaf extract, alpha amylase, alpha glucosidase, plasma insulin, glyburide, acarbose

Abstract

Rauvolfia vomitoria is a medicinal plant that has been implicated by various scientific studies as possessing strong anti-diabetic action; however, the mechanism of action is lacking. Hence this study investigated the anti-diabetic mechanisms of action of Rauvolfia vomitoria through in vitro and in vivo studies. In the in vitro study, the α-amylase and α-glucosidase inhibitory activities of hydromethanolic Rauvolfia vomitoria leaf extract were investigated in comparison with acarbose; while in the in vivo, the effect of the extract on plasma insulin levels of normal and streptozotocin-induced diabetic wistar rats were investigated in comparison with glyburide. Results from the in vitro study showed the percentage inhibition of α-amylase by the extract (100 mg/ml) to be 62.28 (4.73) with an IC50 values of 74.35 mg/ml, while acarbose had a percentage inhibition and IC50 values of 72.81 (2.52) and 66.05μg/ml, respectively. The percentage inhibition of α-glucosidase by the extract (100 mg/ml) was 79.63 (4.09) and an IC50 values of 58.85 mg/ml, while acarbose had a percentage inhibition and IC50 values of 82.11 (1.84) and 56.79μg/ml, respectively. From the in vivo study, the result showed that the extract caused a dose and treatment-duration dependent significant (P<0.05) increase in the plasma insulin levels of streptozotocin-induced diabetic rats in a manner comparable to glyburide. From the results of these investigations, it is therefore concluded that Rauvolfia vomitoria leaf effect its anti-diabetic actions via two separate mechanisms; the plasma insulin increasing mechanism and the α-amylase and α-glucosidase inhibitory mechanism.

Keywords: Rauvolfia vomitoria leaf extract, α-amylase, α-glucosidase, plasma insulin, acarbose, glyburide.

References

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Published

2024-06-30

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Full Length Research Articles

How to Cite

In Vitro and In Vivo Investigation of the Antidiabetic Mechanisms of Action of Rauvolfia vomitoria leaf. (2024). Nigerian Journal of Physiological Sciences, 39(1), 163-169. https://doi.org/10.54548/njps.v39i1.20

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