Ameliorative Effects of Raffia hookeri Pulp Extract on Cisplatin-induced Brain Damage and Consequent Neurobehavioural Changes in Wistar Rats
Parole chiave:
Cisplatin, Raffia hookerii, neuroprotection, neurons, behavioural testsAbstract
Summary: Cisplatin (CIS), a known anticancer drug, has side effects initiated by oxidative damage which hinders its use. Raffia hookeri pulp extract (RHPE), reported to possess antioxidant activity should mitigate cisplatin toxicity. The present study examined the potential of RHPE to reduce brain damage in rats exposed to cisplatin. Forty eight female rats (150 g – 220 g) were randomized into four groups (n = 12) viz: Group 1 served as control received distilled water daily, Group 2 received 100 mg/kg body weight of RHPE, Group 3 received CIS (7.5 mg/kg body weight, intraperitoneally) as single dose, Group 4 received 100 mg/kg body weight of CIS+RHPE. The RHPE was given orally via gavage for 14 days while the single dose of cisplatin was administered on the eighth day of experiment. Behavioral tests namely: transitions, rearings, groomings and forelimb grip strength were carried out on 15th day of the experiment after which rats were euthanized followed by histology and histomorphometry. Cisplatin significantly (p<0.05) reduced the percentage body weight changes, transitions, rearings, groomings and forelimb grip strength compared with the control group, whereas treatment with CIS+RHPE significantly (p<0.05) increased these parameters compared with Cisplatin treatment. Cisplatin also caused histological alterations of Purkinje neurons, pyramidal neurons of Cornu ammonis3, granule cells and cerebral cortex neurons. It significantly (p<0.05) reduced the diameter of Purkinje (9.1±0.59 μm) compared with control (14.41±0.31 μm) and pyramidal neurons (11.32±0.05 μm) compared with control (17.03±0.54 μm). Rats in the CIS+RHPE had their histology considerably improved compared with those of cisplatin. In conclusion, RHPE reversed the behavioural changes and demonstrated neuroprotection against CIS-induced behavioural changes and microanatomical alterations of cerebellar, hippocampal and cerebral neurons
Riferimenti bibliografici
Adaramoye, O.A., Azeez, A.F. and Ola-Davies, O.E. (2016). Ameliorative effects of chloroform fraction of Cocos nucifera L. husk fiber against Cisplatin-induced toxicity in rats. Phcog Res. 8:89-96.
Afifi, A.K. and Bergman, R.A. (2005). Functional neuroanatomy: Text and Atlas, 2nd edition, McGraw–Hill, New. York: 201–222.
Afolayan, A.O., Borokini, T.I. and Afolayan, G.O. (2014). Sublethal Effects of Methanolic Extract of Raphia hookeri on the Reproductive Capacity of Clarias gariepinus. Advances in Zoology. Article ID 615908. Available at: http://dx.doi.org/10.1155/2014/615908. Accessed August 27, 2017.
Akinola, F.F., Oguntibeju, O.O., Adisa, A.W. and Owojuyigbe, O.S. (2010). Physico-chemical properties of palm oil from different palm local factories in Nigeria. J. Food Agric. Environ. 8: 264-269.
Akman, T., Akman, L., Erbas, O., Terek, M.C., Taskiran, D. and Ozsaran, A. (2015). The preventive effect of oxytocin to Cisplatin-induced neurotoxicity: an experimental rat model. Biomed Res Int.2015:167235. DOI: 10.1155/2015/167235
Akpan E.J. and Usoh, I.F. (2004). Phytochemical screening and effect of aqueous root extract of Raphia hookeri (raffia palm) on metabolic clearance rate of ethanol in rabbits. Nigerian Society for Experimental Biology, 16(1): 37 – 42.
Al Moundhri, M.S., Al-Salam, S., Al Mahrouqee, A., Beegam, S. and Ali, B.H. (2012). The effect of curcumin on oxaliplatin and cisplatin neurotoxicity in rats: Some behavioral, biochemical, and histopathological studies. J. Med. Toxicol, doi: 10.1007/s13181-012-0239-x.
Ali, B.H., Ramkumar, A., Madanagopal, T.T., Waly, M.I., Tageldin, M., Al-abri, S., Fahim, M., Yasin, J. and Nemmar, A. (2014). Motor and behavioral changes in mice with Cisplatin-induced acute renal failure Physiol. Res. 63: 35-45.
Amaral, D.G., Scharfman, H.E. and Lavenex, P. (2007). The dentate gyrus: fundamental neuroanatomical organization (dentate gyrus for dummies). Prog Brain Res. 163: 3–22.
Arrieta, O., Hernandez-Pedro, N., Fernadez-Gonzalez-Aragon, M.C., Saavedra-Perez, D., Campos-Parra, A.D., Rios-Trejo, M.A., Ceron-Lizarraga, T., Martinez-Barrera, L., Pinedan, B., Ordonez, G., Ortiz-Plata, A., Granados-Soto, V. and Sotelo, J. (2011). Retinoic acid reduces chemotherapy induced neuropathy in an animal model and patients with lung cancer. Neurology, 77: 987–995.
Bancroft, J.D. and Gamble M. (2008). Theory and Practice of Histology Techniques, 6th edition. Churchill Livingstone Elsevier, Philadelphia: 83 - 134.
Bottone, M.G., Santin G., Piccolin V.M., Bo V.D. and Bernocchi G. (2012). Cisplatin neurotoxicity induces cell death in vitro and in vivo. In: Kojima T, Morita Y (Eds). Cisplatin: Pharmacology, clinical uses and adverse effects. NOVA Science Publishers, Inc.: 123-140.
Chaudhary, M., Joshi, D.K., Tripathi, S., Kulshrestha, S. and Mahdi, A.A. (2014). Docosahexaenoic acid ameliorates aluminum induced biochemical and morphological alteration in rat cerebellum. Ann Neurosci. 21(1): 5-9.
Dada, F.A., Oyeleye, S.I., Ogunsuyi, O.B., Olasehinde, T.O., Adefegha, S.A., Oboh, G. and Boligon AA. (2017). Phenolic constituents and modulatory effects of Raffia palm leaf (Raphia hookeri) extract on carbohydrate hydrolyzing enzymes linked to type-2 diabetes. J Trad. Complem. Med. (2017). Available at: http://dx.doi.org/10.1016/j.jtcme.2017.01.003. Accessed August 03, 2017
Dasari, S. and Tchounwou, P.B. (2014). Cisplatin in cancer therapy: Molecular mechanisms of action. Euro J Pharmacol. Volume 740, 5 October 2014, Pages 364-378. https://doi.org/10.1016/j.ejphar.2014.07.025
Ebokaiwe, A.P., Adedara I.A., Owoeye O, and Farombi E.O. (2013). Neurotoxicity of Nigerian bonny light crude oil in rats. Drug Chem Toxicol, 36 (2): 187-195.
Edelstein, A.D., Tsuchida, M.A., Amodaj, N., Pinkard, H., Vale, R.D. and Stuurman, N. (2014). Advanced methods of microscope control using μManager software. J Biol Meth. 1(2):e11 doi:10.14440/jbm.2014.36
Edem, D.O., Eka, O.U. and Ifon, E.T. (1984). Chemical evaluation of the nutritive value of the raffia palm fruit (Raphia hookeri) Food Chemistry. 15(1): 9-17
Ellis, H. (2006). Clinical Anatomy: Applied Anatomy for students and junior doctors. Blackwell, Oxford, UK: 349-352.
Folarin, O.R., Snyder, A.M. Peters, D.G., Olopade, F., Connor, J.R. and Olopade, J.O. (2017). Brain Metal Distribution and Neuro-Inflammatory Profiles after Chronic Vanadium Administration and Withdrawal in Mice. Front. Neuroanat. 11:58. doi: 10.3389/fnana.2017.00058
Fonnum, F. and Lock, E.A. (2000). Cerebellum as a target for toxic substances. Toxicol Lett. 15: 112–113: 9–16.
Gregg, R.W., Molepo, J.M., Monpetit, V.J., Mikael, N.Z., Redmond, D., Gadia, M, and Srewart, D.J. (1992). Cisplatin neurotoxicity: the relationship between dosage, time, and platinum concentration in neurologic tissues, and morphologic evidence of toxicity. J. Clin. Oncol. 10(5): 795-803.
Gulec, M., Oral, E., Dursun, O.B., Yucel, A., Hacimuftuoglu, A., Akcay, F. and Suleyman H. (2013). Mirtazapine protects against cisplatin-induced oxidative stress and DNA damage in the rat brain. Psych. Clin. Neurosci. 67: 50–58
Hashem, R.M., Safwat, G.M., Rashed, Laila, A. and Bakry, S. (2015). Biochemical findings on cisplatin-induced oxidative neurotoxicity in rats. Int. J. Adv. Res. 3(10): 1222 – 1231.
Karavelioglu, E., Boyaci, M.G., Simsek, N., Sonmez, M.A., Koc, R., Karademir, M., Guven, M. and Eser, E. (2015). Selenium protects cerebral cells by cisplatin induced neurotoxicity. Acta Cirúrgica Brasileira. 30(6): 394-400.
Kim, B.J., Lee, S.Y., Kim, H.W., Park, E-J., Kim, J., Kim, S.J., So, I. and Jeon, J-H. (2009). Optimized Immunohistochemical Analysis of Cerebellar Purkinje Cells Using a Specific Biomarker, Calbindin D28k. Korean J Physiol Pharmacol. 13: 373－378.
Ko,,J-W., Lee, I-C., Park, S-H., Moon, C., Kang, S-S., Kim, SH. and Kim, J-C. (2014). Protective effects of pine bark extract against cisplatin-induced hepatotoxicity and oxidative stress in rats. Lab Anim Res 2014: 30(4), 174-180.
Kumar, P., Sulakhiya, K., Barua, C.C. and Mundhe, N. (2017). TNF-α, IL-6 and IL-10 expressions, responsible for disparity in action of curcumin against cisplatin-induced nephrotoxicity in rats. Mol Cell Biochem. 431:113-122.
Mbaka, G.O., Ogbonnia, S.O., Oyeniran, K.J. and Awopetu, P.I. (2012). Effect of Raphia hookeri Seed Extract on Blood Glucose, Glycosylated Haemoglobin and Lipid Profile of Alloxan Induced Diabetic Rats, Br. J. Med. Med. Res., 2(4): 621-635.
Mohammad, S., Shahrnaz, P., Masoud, N., Moazamehosadat, R., Nasser, Z., Khadije, E. and Fatemeh, A. (2010). Walnut consumption protects rats against cisplatin - induced neurotoxicity. Neurotoxicol.oi:10.1016/j.neuro.2012.08.004. Accessed 02/11/2014.
Mokhtari, M.J., Akbarzadeh, A., Hashemi, M., Javadi, G., Reza, R., Mehrabi, M.R., Farhangi, A.
and Mohammadi, H. (2012). Cisplatin Induces Down Regulation of BCL2 in T47D Breast Cancer Cell Line. Adv. Stud. Biol., 4(1): 19 – 25.
Oduah, A.A. and Ohimain, E.I. (2015). Ethnobotany of raffia palm (Raphia hookeri), productivity assessment and characterization of raffia palm oil from the Niger Delta, Nigeria Res. J. Phytomed. 01[01] 2015. Available at: www.asdpub.com/index.php/rjp (Online). Accessed August 02, 2017
Owoeye, O. and Onwuka, S.K. (2015). Tomato pomace powder ameliorated cisplatin-induced microanatomical alterations in brain of Wistar rats. Int. J. Biol. Chem. Sci. 9(1):1-11.
Owoeye, O., Femi-Akinlosotu, O.M. and Adejuwon S.A. (2015). Launae taraxacifolia aqueous extract attenuates Cisplatin-induced neurotoxicity, by decreasing oxidative stress and neuronal cell death in rats. Arch. Basic Appl. Med. 3:71-78.
Polterait, O. (1997). Antioxidants and free-radical scavengers of Natural Origin. Current. Org. Chem. 1: 415-440.
Public Health Service (PHS) 1996. Public health service policy on humane care and use of laboratory animals. US Department of Health and Human Services, Washington, DC, PL. 99-158.
Sanchez-Gonzalez, P.D., Lopez-Hernandez, F.J., Lopez-Novoa, J.M. and Morales, A.I. (2011). An integrative view of the pathophysiological events leading to cisplatin nephrotoxicity. Crit Rev Toxicol 41: 803-821. Stepan, J., Dine, J. and Eder, M. (2015). Functional optical probing of the hippocampal trisynaptic circuit in vitro: network dynamics, filter properties, and polysynaptic induction of CA1 LTP. Front Neurosci. 9: 160. Tamashiro, K.L.K., Wakayama, T., Blanchard, R.J., Blanchard, C. and Yanagimachi, R. (2000). Postnatal growth and behavioral development of mice cloned from adult cumulus cells. Biol Reprod. 63:328–334. Turan, M.I., Turan, I.S., Mammadov, R., Altinkaynak, K. and Kisaoglu, A. (2013). The Effect of Thiamine and Thiamine Pyrophosphate on Oxidative Liver Damage Induced in Rats with Cisplatin. BioMed Research International, Article ID 783809: 1-6.
Dowloads
Pubblicato
Fascicolo
Sezione
Licenza
Questo volume è pubblicato con la licenza Creative Commons Attribuzione - Non commerciale - Non opere derivate 4.0 Internazionale.
