Safety Evaluation of Osun River Water Containing Heavy Metals and Volatile Organic Compounds (VOCs) in Rats
Mots-clés :
Osun river, Haematology, Biochemical parameters, Heavy metals, Volatile Organic CompoundsRésumé
Summary: This study evaluated the pH, heavy metals and volatile organic compounds (VOCs) in Osun river water. It also evaluated its safety in rats. Heavy metals were determined by atomic absorption spectrophotometry (AAS) while VOCs were determined by gas chromatography coupled with flame ionization detector (GC-FID). Male and female rats were exposed to Osun river water for three weeks and then sacrificed. The abundance of heavy metals in Osun river followed the trend Pb > Cd > Zn > Fe > Cr > Cu while VOCs followed the trend benzene < ethylbenzene < toluene < xylene. The concentrations of Pb, Cd and benzene were higher than the permissible limits of Standards Organization of Nigeria (SON) and World Health Organization (WHO) respectively. Rats exposed to Osun river water for three weeks had increased WBC, thiobarbituric acid reactive substances (TBARS), serum proteins and serum aminotransferases. There were also significant decreases (P < 0.05) in HCT, PLT, liver aminotransferases and liver glutathione compared to the control. These results show that the pollutants in Osun river water are capable of inducing hematological imbalance and liver cell injury. The toxicity induced in blood was sex-dependent affecting female rats more than male rats.
Références
Ajiboye, T. O., Adeleye, A. O., Salau, A. K, Ojewuyi, O., Adigun, N. S., Sabiu, S. and Sunmonu, T. O. (2014). Phenolic extract of Parkia biglobosa fruit pulp stalls aflatoxin B1-mediated oxidative rout in the liver of male rats. Revista Brasileira de Farmacognosia –Braz. J. Pharmacog. 24; 668-676.
Akanji, M. A., Salau, A. K. and Yakubu, M. T. (2013). Safety evaluation of aqueous extract of Crateva adansonii leaves on selected tissues of rats. Fountain J. Nat. Appl. Sci. 2(1); 17-28.
Azeez, L., Oyedeji, O.A., Abdulsalami, I.O. and Adewuyi, S.O. (2013). Characterization of odourous compounds in air, leachate, stream and well in and around Taju-Bello Dumpsite, Lagos, Nigeria. Adv. Env. Res. 2(2); 143-153
Buege, J.A. and Aust, S.D. (1978). Microsomal lipid peroxidation. Methods Enzymol. 52; 302–310.
Edewor, T.I., Olajire, A.A. and Olaniyan, L.E.B. (2007). Effects of oral administration of ethanolic leaf extract of Acanthospermum hispidum DC on carbon tetrachloride induced acute liver injury in rats. Res. J. Med. Sci. 1(1); 39-41.
Eletta, O.A.A. (2012). Water Quality Monitoring and Assessment in a Developing Country, Water Quality Monitoring and Assessment. 481-495. Available from:
El-Gendy, K.S., Aly, N.M., Mahmoud, F.H., Kenawy, A., El-Sebae, A.K.H. (2010). The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid. Food & Chem. Toxico. 48; 215–221
Elkind, M.S., Cheng, J., Boden-Albala, B., Paik, M.C. and Sacco, R.L. (2001). Elevated White Blood Cell Count and Carotid Plaque thickness: The Northern Manhattan Stroke Study. Stroke. 32;842-849
Ellman, G.L. (1959). Tissue sulfhydryl groups. Arch. Biochem. Biophysic 82;70–77.
Eyong E.U., Umoh I.B., Ebong P.E., Eteng M.U., Antai A.B. and Akpa A.O. (2004). Haematoxic effects following ingestion of Nigerian Crude oil and crude oil polluted shellfish by rats. Nig. J. Physiol. Sci. 19(1-2); 1 – 6
Gornall, A.C., Bardawill, C.J. and David M.M. (1949). Determination of serum protein by means of Biuret reaction. J. Bio. Chem. 177; 751- 766.
Jadhav, S.H., Sarkar, S.N., Patil, R.D. and Tripathi, H.C. (2007). Effects of subchronic exposure via drinking water to a mixture of eight water-contaminating metals: a biochemical and histopathological study in male rats. Arch. Env. Contam. Toxico. 53; 667-677.
Jassim, H.M. and Hassan, A.A. (2011). Changes in some blood parameters in lactating female rats and their pups exposed to lead: Effects of Vitamins C and E. Iraqi J. Vet. Sci. 25 (1); 1-8
Jee, S.H., Park, J.Y., Kim, H.S., Lee, T.Y., and Samet, J.M. (2005). White Blood Cell count and risk for all-cause, Cardiovascular, and Cancer mortality in a cohort of Koreans. Am. J. Epidem. 162; 1062-1069
Kori-Siakpere, O. and Ikomi, U. (2011). Alterations in Some Haematological Parameters of the African Snakehead: Parachanna Africans Exposed to Cadmium. Notulae Sci. Biol. 3 (4); 29-34.
Mansour, S.A., Mossa, A.H. and Heikal, T.M. (2007). Haematoxicity of a New Natural Insecticide "Spinosad" on Male Albino Rats. Int. J. Agric. & Bio. 9(2), 342-346
Mayne P.D. (2005). Clinical Chemistry in Diagnosis and Treatment. 6th Ed. Lloyd-luke (Medical Books) Ltd
NIH. (1985). Guide for the care and use of laboratory animals, NIH publication.
Nikolaou, A.D., Golfinopoulos, S.K., Kostopoulou, M.N., Kolokythas, G.A., Lekkas, T.D. (2002). Determination of volatile organic compounds in surface waters and treated wastewater in Greece. Water Res. 36; 2883-2890.
Nkolika, I.C. and Benedict, O.C.O. (2009). Elevated Cadmium Levels in Blood of the Urban Population in Enugu State Nigeria. World Appl. Sci. J. 7(10); 1255-1262.
Olajire, A.A. and Imepekperia, F.E. (2000). A study of water quality of the Osun River; metal monitoring and Geochemistry. Bull. Chem. Soc. Ethiopia. 14(1); 1-18.
Olajire, A.A. and Imepekperia, F.E. (2001). Water quality assessment of Osun river: studies on inorganic nutrients. Env. Mon. & Ass.69; 17-28
Olajire, A.A and Azeez, L. (2012). Effects of solanum macrocarpon (African eggplant) on haematological parameters of wistar rats exposed to urban air pollution. Adv.Env. Res. 1(2); 109-123.
Pramyothin, P., Samosorn P., Poungshompoo, S. and Chaichantipyuth C. (2006). The protective effects of Phyllanthus emblica Linn extract on ethanol induced rat hepatic injury. J. Ethnopharmacol. 107; 3, 361 – 364.
Rechenmacher, C., Siebel, A.M., Goldoni, A., Klauck, C.R., Sartori, T., Rodrigues, M.T. Rodrigues, M.A.S., Gehlen, G., Ardenghi P.G. and Silva, L.B.A. (2010). Multibiomarker approach in rats to assess the impact of pollution on Sinos River, southern Brazil.Braz. J. Bio.70 (4), 1223-30.
Reitman, S. and Frankel. S.A. (1957). A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Path. 28, 56-63.
Saadat, M. and Bahaoddini, A. (2004). Hematological changesdue to chronic exposure to natural gas leakage in polluted areas of Masjid-i-Sulaiman. Eco. &Env. Safety. 58; 273–276
Salau, A.K., Yakubu, M.T., Oladiji, A.T. (2013). Cytotoxic activity of aqueous extracts of Anogeissus leiocarpus and Terminalia avicennioides root barks against Ehrlich Ascites arcinoma cells. Ind. J. Pharmacol. 45; 381 – 385
Salau, A.K., Yakubu, M.T., Oluleye, D.S., Oloyede, O.B. and Akanji, M.A. (2012). Toxicological evaluation of aqueous leaf extract of Ficus exasperata on selected tissues of normal Wistar rats. Centrepoint J. (Science Edition). 18(1); 55-66
Salau, A. K, Yakubu, M. T. & Oladiji, A. T. (2016) Effects of aqueous root bark extracts of Anogeissus leiocarpus (DC) Guill & Perrand Terminalia avicennioides Guill & Perr on redox and haematological parameters of diethylnitrosamine-administered rats. Iranian J. Toxicol. 10(1) 21-29.
Salau, A. K. (2013) Anticancer Activity and Toxicological Effects of Aqueous Extracts of Anogeissus leiocarpus and Terminalia avicennioides Root Barks. Ph. D. Thesis, Department of Biochemistry, University of Ilorin, Ilorin, Nigeria (Unpublished).
Sallau, A.A., Ekanem. E.O., Abubakar, A.B., (2011). Some toxic heavy metals in soil of municipal solid waste dumpsite in Bauchi metropolis. 34th Annual International Conference of The Chemical Society of Nigeria (CSN) at Unilorin., EN001-EN007
Uboh, F.E., Akpanabiatu, M.I., Eteng, M.U., Ebong, P.E., Umoh, I.B., (2008a). Toxicological effects of exposure to gasoline vapour in male and female rats. The Int. J. Toxico. 4(2). DOI: 105580/1ca
Uboh, F., Akpanabiatu, M., Ebong, P., Umoh, I., (2008b). Gender differences in the haematotoxicity and weight changes associated with exposure to gasoline vapours in wistar rats. The Int.J. Toxico. 5(2); DOI: 105580/1153
Wahab, M.K.A., Ganiyu, O.T., Olabiran, A.D., Ojo, S.D., (2012). Prevalence of pathogenic microorganism in Osun Osogbo sacred river water. Int. J. Agric. Sci. 2(10); 935-941.
WHO 1998). Basic OECD principles of GLP, Geneva, Switzerland.
Yakubu, M.T., Akanji, M.A., Oladiji, A.T., (2005). Aphrodisiac potentials of the aqueous extract ofFadogia argresits (Schweinf. Ex Hiern) stem in male albino rats. Asian J. Adrol.7; 399-404.
Yakubu, M.T. and Musa I.F (2012). Liver and kidney functional indices of pregnant rats following the administration of the crude alkaloids from Senna alata (Linn Roxb) leaves. Ind. J. Toxico. 6(16); 615-625
Yilmaz, M.B., Saricam, E., Biyikoglu, S.F., Guray, Y., Guray, U., Sasmaz, H. and Korkmaz, S., (2004). Mean platelet volume and exercise stress test. Thrombolysis. 17(2); 115-1120
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