Cobalt Modulates Healing of Gastric Ulceration in Male Wistar Rats: iNOS/COX-2 Crosstalk

Authors

  • A.T. SALAMI
  • U. AKPAMU
  • T.O. IYIOLA
  • S.B. OLALEYE

DOI:

https://doi.org/10.4314/

Keywords:

Reserpine induced Gastric ulcer, Pyloric ligation gastric ulcer model, Cobalt chloride, antioxidant assay, mast cells

Abstract

Background: Information on the interplay between gastric iNOS and COX-2 expression by Cobalt (CoCl2) during gastro-protection is unclear.

Objective: Cobalt activities on iNOS and COX-2 interplay was investigated using Reserpine (RGU), and Pyloric-ligation (PLGU) gastric ulcer models.

Materials and Methods: In RGU, 50 Wistar rats grouped; 1-control, 2-ulcerated untreated (RUU), 3, 4, and 5 were ulcerated pre-treated with 62mg/kg, 25mg/kg CoCl2 and 3.6mg/kg omeprazole for 5 days and sacrificed 3 and 8 hours after reserpine administration. In PLGU, 25 Wistar rats grouped; A-25mg/kg Cocl2, B-62mg/kg Cocl2, C-Cimetidine (36.88mg/kg), D-ulcerated untreated (UU), and E–control, were pretreated for 8 days prior 18 hours pyloric-ligation. Thereafter, macroscopic gastric ulceration (GU), biochemical analysis with histology, and immunohistochemistry were evaluated on excised stomach. Data were expressed as Mean+SEM, and p<0.05 was significant.

Results: In RGU model, significant increase in GU score, nitric oxide, lipid peroxidation, and degranulated mast cells but a decrease in sulfhydryl, hydrogen peroxide levels in RUU by 3H and 8H were ameliorated by CoCl2. Histology revealed increased degranulated mast cells in the RUU compared with CoCl2-treated groups. CoCl2 treatment significantly decreased gastric acid secretion, ulcer index, parietal cell count, H+/K+ATPase and Na+/K+ATPase activities compared with UU. Gastric sulfhydryl, mucin, pepsin, with mucous cell count significantly increased in CoCl2-treated groups compared with UU. Gastric iNOS positive reactivity was upregulated while COX-2 positive reactivity was downregulated in CoCl2-treated groups compared with UU .

Conclusion: Cobalt chloride exerted gastrointestinal cytoprotective action via mast cell stabilization, mucus production, with modulated expressions of gastric iNOS and COX-2 reactivity at the ulcerated site.

Effect of cobalt on gastric biochemical assay (mucin, Nitric oxide, H+K+ ATPase and Na+K+ ATPase activities) during Pyloric ligation Induced gastric ulcer model

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Published

2025-01-20

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How to Cite

Cobalt Modulates Healing of Gastric Ulceration in Male Wistar Rats: iNOS/COX-2 Crosstalk. (2025). Nigerian Journal of Pharmaceutical Research, 20(2), 167-184. https://doi.org/10.4314/

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