Fungal Profile and Pathogenicity Indices Associated with Cucumber Fruits in Aba, Abia State, Nigeria.

Authors

  • Dr. Christian Chukwuemeka Ike University of Agriculture and Environmental Sciences, Umuagwo, Imo State
  • Dr. H. O. Ogwuegbu Microbiology Department, Spiritan University, Nneochi, Umunneochi, Abia State

DOI:

https://doi.org/10.4314/ajbr.v27i2.17

Keywords:

Cucumber, Fungal Profile, Safety, Pathogenicity, Severity Index

Abstract

The fungal profile and pathogenicity indices associated with cucumber fruits were determined using standard microbiological methods. A total of one hundred and twenty-five (125) samples were randomly picked from five (5) different markets/ hawking points, transported in ice bags to Laboratory for analysis, prepared and made ready for microbiological analysis. Also pathogenicity tests were conducted on the identified isolates to establish both severity and percent disease index in the Koch postulates of disease development. The mean fungal count ranged from 1.0 x 103 ±0.25/±0.32 to 5.4 x 103 ±0.12. Isolates were characterized colonially, microscopically, and morphologically with their identities confirmed with reference chart. The four (4) isolates include Aspergillus species, Penicillium species, Fusarium species, and Cladosporium species. Due to high microbial thresholds of associated genus, there is need for all round awareness to avoid health outbreaks and pathogenesis tendencies, as some isolated species could produce potent toxins that are linked with food-borne illnesses. Bruises, pierces and surface wounds should be avoided at all times, having known the PDI of some isolates. Infestation by rodents and insects during storage should be avoided as it may constitute serious health and deterioration exposures. Washing of fruits with salted-water before eating is most recommended, as transit eating of any kind should be discouraged.

Author Biography

  • Dr. H. O. Ogwuegbu, Microbiology Department, Spiritan University, Nneochi, Umunneochi, Abia State

    Senior Lecturer, Microbiology Department, Spiritan University, Nneochi, Umunneochi, Abia State

References

References

Abbey, S.D. (2006). Foundation in Medical Mycology, 4th edition. Kenalf Publication, Port Harcourt, Nigeria, pp. 22-30.

Abiodun, O. A. (2010). Comparative studies on nutritional composition of four melon seeds varieties. Pakistan Journal of Nutrition, 9 (9), 905-908.

Adam, K.L. (2006). Squash bug and squash vine borer. International Journal of Food Microbiology, 93,141-154.

Cappucino, G. J. R & Sherman, B. (2010). Microbiology: A Laboratory Manual, 9th Edition. The Benjamin Publishing Company. California.

Efiuvwevwere, B. J. O. (2000). Microbial Spoilage agents of Tropical and assorted Fruits and Vegetables: An illustrative reference book. Second edition, Paragraphics Publishers, Port Harcourt, Nigeria, pp. 4-6.

Food Standards Australia New Zealand [FSANZ]. (2018). Compendium of Microbiological Criteria for Food.

Freeman, S., Katan, T. & Shabi, E. (1998). “Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits.” Plant Disease, 82(6), 596–605.

Guarnaccia, V., Vitale, A., Cirvilleri, G., Aiello, D., Susca, A., Epifani, F., Perrone, G. & Polizzi, G. (2016). Characterisation and pathogenicity of fungal species associated with branch cankers and stem-end rot of avocado in Italy. European Journal of Plant Pathology, 146(4), 963-976

Ike, C.C. & Ogwuegbu, H.O. (2020). Microbial quality of locally processed suya sold in Owerri metropolis, Imo State, Nigeria. GSC Biological and Pharmaceutical Sciences, 12(3), 044-050.

Ike, C.C., Emeka-Ike, P.C. & Akortha, E. E. (2017). Microbial evaluation of tigernut (Cyperus esculentus L.) sold in Aba, Abia State, Nigeria. International Journal of Research and Development Organization (IJRDO) – Journal of Biological Science, 3(5), 97-107.

Ike, C.C., Emeka-Ike, P.C., Nwokorie, C.C. & Anochie, C.C. (2015). Microbiological quality evaluation of locally prepared snacks sold in Aba metropolis, Abia State, Nigeria. International Journal of Scientific Engineering and Applied Science (IJSEAS), 1(7), 46-59.

Jay, M.J., Loessner, M. J. & Golden, D.A. (2005). Modern Food Microbiology, Seventh Edition. Springer Publishers, USA.

John, W.C. & Mullinix, B.G. (2008). Cultural control of yellow nutsedge (Cyperus esculentus) in transplanted cantaloupe (Cucumis melo) by varying application timing and of thin film mulches. Crop Protection Journal, 27, 735-739.

Kidd, S., Halliday, C., Alexiou, H. & Ellis, D. (2016). Description of Medical Fungi. 3rd edition. Newstlye Printing. Adelaide, South Australia 5031, Australia, 23-170.

Lakshmi, B.K.M., Reddy, P.N. and Prasad, R.D. (2011). “Cross-infection potential of Colletotrichum gloeosporioides Penz. Isolates causing anthracnose in subtropical fruit crops,” Tropical Agricultural Research, 22(2), 183–193.

Lee D.H., Iwanski G.B. & Thoennissen, N.H., (2010). Cucurbitacin ancient compound shedding new light on cancer treatment. Scientific World Journal, 10, 413-418.

Mayee, C.D. & Datar, V.V. (1986). Phytopathometry. Marathwada Agricultural University, Parbhani, pp 95.

Public Health Laboratory Service [PHLS]. (2000). Guidelines for the microbiological quality of some ready-to-eat foods sampled at the point of sale. Communicable Disease & Public Health, 3, 163-7.

Singh, J. (2010). Free radical scavenging and analgesic activities of Cucumis sativus l, fruit extract. American Journal of Toxicological Sciences, 2(1), 43-50.

Twizeyimana, M., Förster, H., McDonald, V., Wang, D. H., Adaskaveg, J. E. and Eskalen, A. (2013). “Identification and pathogenicity of fungal pathogens associated with stem-end rot of avocado in California,” Plant Disease, 97(12), 1580–1584.

Watanabe, T. (2010). Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species, Third Edition. CRC Press. pp 211 – 376.

Wanjiku, E.K., Waceke, J.W., Wanjala, B.W. & Mbaka, J.N. (2020). Identification and Pathogenicity of Fungal Pathogens Associated with Stem End Rots of Avocado Fruits in Kenya. International Journal of Microbiology, 2020, 1-8

Downloads

Published

2024-05-31

Issue

Section

Original Articles

How to Cite

Fungal Profile and Pathogenicity Indices Associated with Cucumber Fruits in Aba, Abia State, Nigeria. (2024). African Journal of Biomedical Research, 27(2), 325-329. https://doi.org/10.4314/ajbr.v27i2.17

Similar Articles

1-10 of 74

You may also start an advanced similarity search for this article.