Cnidoscolus aconitifolius Extract Represses Nociception Via Opiodergic And Cholinergic Pathways In Laboratory Mice
DOI:
https://doi.org/10.4314/Keywords:
Cnidoscolus aconitifolius, nociception, opiodergic system, cholinergic system, adrenergic systemAbstract
Cnidoscolus aconitifolius plant has been shown to have antinociceptive effect, but the underlying mechanisms are unknown. This study examined the antinociceptive mechanisms of action C. aconitifolius by probing the opiodergic, cholinergic, and adrenergic systems. Behavioural assessments of pain were done using the paw licking test caused by formalin, abdominal writhing induced by acetic acid and tail-flick tests. Methanol extract of C. aconitifolius (MECA) significantly reduces nociception in the paw licking ibduced by formalin and abdominal writhing induced by acetic acid but not tail flick test. Pretreatment with opoidergic and cholinergic blockers reversed these effects but not adrenergic blocker. MECA can be said to potentiate its antinociceptive effect by acting peripherally in the manner of opioidergic and cholinergic agents.
References
Alexa-Stratulat, T. et al . (2017) ‘Nutritional Modulators in Chemotherapy-Induced Neuropathic Pain’, in Nutritional Modulators of Pain in the Aging Population. doi: 10.1016/B978-0-12-805186-3.00002- 3.
Amos, S. et al . (2001) ‘Neuropharmacological effect of the aqueous extract of Sphaeranthus senegalensis in mice’, Journal of Ethnopharmacology, 78(1). doi: 10.1016/S0378-8741(01)00316-6.
Anilkumar, M. (2010) ‘Ethnomedicinal plants as anti-inflammatory and analgesic agents’, Ethnomedicine: A Source of Complementary Therapeutics, 661(2).
Le Bars, D., Gozariu, M. and Cadden, S. W. (2001) ‘Animal models of nociception’, Pharmacological Reviews. doi: 10.1002/9783527611942.ch9.
Collier, H. O. et al . (1968) ‘The abdominal constriction response and its suppression by analgesic drugs in the mouse.’, British journal of pharmacology and chemotherapy, 32(2). doi: 10.1111/j.1476- 5381.1968.tb00973.x.
Coluzzi, F. et al . (2017) ‘The challenge of perioperative pain management in opioid-tolerant patients’,
Therapeutics and Clinical Risk Management. doi: 10.2147/TCRM.S141332.
Crofford, L. J. (2013) ‘Use of NSAIDs in treating patients with arthritis’, Arthritis Research and Therapy. doi: 10.1186/ar4174.
D’Amour, F. and Smith, D. (1941) ‘A METHOD FOR DETERMINING LOSS OF PAIN SENSATION’, The Journal of Pharmacology and Experimental Therapeutics, 72(1).
Figueredo, S. M. et al . (2011) ‘Antinociceptive and gastroprotective actions of ethanolic extract from Pluchea sagittalis (Lam.) Cabrera’, Journal of Ethnopharmacology, 135(3). doi: 10.1016/j.jep.2011.03.006.
Gawade, S. P. (2012) ‘Acetic acid induced painful endogenous infliction in writhing test on mice’,
Journal of Pharmacology and Pharmacotherapeutics. doi: 10.4103/0976-500X.103699.
Hayashida, K. I. and Eisenach, J. C. (2010) ‘Spinal α2-adrenoceptor-mediated analgesia in neuropathic pain reflects brain-derived nerve growth factor and changes in spinal cholinergic neuronal function’, Anesthesiology, 113(2). doi: 10.1097/ALN.0b013e3181de6d2c.
Hayashida, K. I. and Obata, H. (2019) ‘Strategies to treat chronic pain and strengthen impaired descending noradrenergic inhibitory system’, International Journal of Molecular Sciences. doi: 10.3390/ijms20040822.
Hong, Y. and Abbott, F. V. (1995) ‘Peripheral opioid modulation of pain and inflammation in the formalin test’, European Journal of Pharmacology, 277(1). doi: 10.1016/0014-2999(95)00045-M.
Ikeda, Y. et al . (2001) ‘Involvement of vanilloid receptor VR1 and prostanoids in the acid-induced writhing responses of mice’, Life Sciences, 69(24). doi: 10.1016/S0024-3205(01)01374-1.
Jiang, Y. Y. et al . (2018) ‘Cholinergic neurons in medial septum maintain anxiety-like behaviors induced by chronic inflammatory pain’, Neuroscience Letters, 671. doi: 10.1016/j.neulet.2018.01.041.
Labuz, D. et al . (2016) ‘Distinct roles of exogenous opioid agonists and endogenous opioid peptides in the peripheral control of neuropathy-triggered heat pain’, Scientific Reports, 6. doi: 10.1038/srep32799.
Mackey, S. and Kao, M. C. (2019) ‘Managing twin crises in chronic pain and prescription opioids’, BMJ (Online). doi: 10.1136/bmj.l917.
NIH (1996) ‘Guide for the Care and Use of Laboratory Animals’, Guide for the Care and Use of Laboratory Animals. doi: 10.17226/5140.
Nwafor, P. A. and Okwuasaba, F. K. (2003) ‘Anti-nociceptive and anti-inflammatory effects of methanolic extract of Asparagus pubescens root in rodents’, Journal of Ethnopharmacology, 84(2–3). doi: 10.1016/S0378-8741(02)00213-1.
Onasanwo, S. A., Oyagbemi, A. A. and Saba, A. B. (2011) ‘Anti-inflammatory and analgesic properties of the ethanolic extract of Cnidoscolus aconitifolius in rats and mice’, Journal of Basic and Clinical Physiology and Pharmacology, 22(1–2). doi: 10.1515/JBCPP.2011.010.
Owemidu, I. et al . (2018) ‘Anti-nociceptive and anti- inflammatory activities of the methanol extract of Waltheria americana leaf in experimental animals.’, Journal of Complementary Medicine Research,
(2). doi: 10.5455/jcmr.20180118112751.
Ross-Ibarra, J. and Molina-Cruz, A. (2002) ‘The ethnobotany of chaya (Cnidoscolus aconitifolius ssp. aconitifolius Breckon): A nutritious Maya vegetable’, Economic Botany, 56(4). doi: 10.1663/0013- 0001(2002)056[0350:TEOCCA]2.0.CO;2.
Salvemini, D. et al . (1995) ‘Dual inhibition of nitric oxide and prostaglandin production contributes to the antiinflammatory properties of nitric oxide synthase inhibitors’, Journal of Clinical Investigation,
(1). doi: 10.1172/JCI118035.
Sen, S. and Chakraborty, R. (2017) ‘Revival, modernization and integration of Indian traditional herbal medicine in clinical practice: Importance, challenges and future’, Journal of Traditional and Complementary Medicine. doi: 10.1016/j.jtcme.2016.05.006.
Srinivasan, K. et al . (2003) ‘Antinociceptive and antipyretic activities of Pongamia pinnata leaves’,
Phytotherapy Research, 17(3). doi: 10.1002/ptr.1126.
Świeboda, P. et al . (2013) ‘Assessment of pain : types , mechanism and treatment’, (July 2014).
Taber, R. I. et al . (1969) ‘Agonist and antagonist interactions of opioids on acetic acid-induced abdominal stretching in mice.’, Journal of Pharmacology and Experimental Therapeutics, 169(1).
Tamba, B. I. and Alexa-Stratulat, T. (2017) ‘Trace Elements Alleviate Pain in Mice and Humans’, in
Nutritional Modulators of Pain in the Aging Population. doi: 10.1016/B978-0-12-805186-3.00017-5.
Yashpal, K. and Coderre, T. J. (1998) ‘Influence of formalin concentration on the antinociceptive effects of anti-inflammatory drugs in the formalin test in rats: Separate mechanisms underlying the nociceptive effects of low- and high-concentration formalin’, European Journal of Pain, 2(1). doi: 10.1016/S1090-3801(98)90047-7.
Yin, W. et al . (2003) ‘Analgesic and anti-inflammatory properties of brucine and brucine N-oxide extracted from seeds of Strychnos nux-vomica’, Journal of Ethnopharmacology, 88(2–3). doi: 10.1016/S0378-8741(03)00224-1.
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