Effects of Silver Nanoparticle Modified Corchorus olitorius (Jute) Leaf Extract on Blood Sugar and Lipid Profile of Diabetic Wistar Rats
DOI:
https://doi.org/10.4314/ajbr.v27i2.26Keywords:
Cochorus olitorius leaf, phytochemicals, silver nanoparticles, antidiadetic, blood lipidsAbstract
Diabetes, a high prevalent and non-infectious disease characterized with expensive management coupled with its complication exerts serious economic burden on patients and healthcare practitioners. This study aimed at evaluating the effects of silver nanoparticles modified Corchorus olitorius leaf extracts (AgNPs-COLE) on blood sugar and lipid profile of alloxan induced diabetic wistar rats. Phytochemical screening of Corchorus olitorius leaf extract (COLE) was carried out using gravimetric method. The energy dispersive x-ray fluorescence spectrometer was used to determine the elemental content of green synthesized silver nanoparticles. Thirty five (35) Wistar rats of 184 – 201g were used and grouped into A-G of 5 per group. Group A were non-induced and not treated except feeding only, Groups B-G were induced with diabetes using 130mg/kg alloxan monohydrate, while Group B were not treated, group C were administered 10mg/kg glibenclamide (GLB), groups D and E were administered with aqueous leaf extracts of Cochorus olitorius of 200mg/kg and 400mg/kg, respectively, and groups F and G were administered with 200 mg/kg and 400mg/kg of AgNPs modified Jute leaf extract, respectively. The blood sugar level and lipids assay were determined. Data analysis was carried out using multivariate ANOVA of SPSS version 26 with inferior p-value of 0.05. Alkaloid, flavonoid, terpernoids, steroid and saponins were predominant phytochemicals with yields of 16.46, 11.42, 6.9, 3.58 and 2.42 %. Blood sugar levels of the rats were significantly reduced in all groups following treatment regime. Total cholesterol, TG, HDL-C and LDL-C levels of diabetic rats significantly reduced when administered with AgNPs-COLE compared to COLE and GLB, respectively. Thus, silver nanoparticles enhanced anti-diabetogenic potentials of C. olitorius leaf extract and performed better than glibenclamide in diabetes management.
References
Adeli, K., Taghibiglou, C. and Van Iderstine, S. C. (2001). Mechanisms of hepatic very low-density lipoprotein overproduction in insulin resistance. Trends in Cardiovacular. Medicine, 11(5), 170–176.
Ahmed, Z. and Sarkar, S. (2022). review on jute leaf: A powerful biological tool. International Journal of Scientific Research Updates, 4(01), 064–085.
Ahmed, Z., Banu, H., Akhter, F. and Izumori, K. (2001). A simple method for D-xylose extraction from jute stick and rice husk. Online Journal of Biological Science, 1 (11), 1001-1004.
Ahmed, S., Ahmad, M., Swami, B. L. and Ikram, S. (2016). A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. Journal of Advanced Research, 7, 17 - 28. https://doi.org/10.1016/j.jare.2015.02.007
Borradaile, N. M., de Dreu, L. E., Barrett, P. H., Behrsin, C. D. and Huff, M. W. (2003). Hepatocyte apoB containing lipoprotein secretion is decreased by the grapefruit flavonoid, naringenin, via inhibition of MTP mediated microsomal triglyceride accumulation. Biochemistry, 42(5), 1283-1291.
Chao, P. D. L., Hsiu, S. L. and Hou, Y. C. (2002). Flavonoids in herbs: Biological fates and potential interactions with xenobiotics. Journal of Food Drug and Anal, 10(4), 219-228.
Diniz, Y. S. A, Cicogna, A. C, and Padovani, C. R. (2004). Diets rich in saturated and polyunsaturated fatty acids: metabolic shifting and cardiac health. Nutrition, 20, 230–234.
Elekofehinti, O. O., Adanlawo, I. G., Saliu, J. A. and Sodehinde, S. A. (2012). Saponins from Solanum anguivi fruits exhibit hypolipidemic potential in Rattus novergicus. Der Pharmacia Lettre. 4 (3), 811-814.
Fossati, P., Prencipe, L., and Lorenzo P. (1982). Serum triglycerigle determination colourimetrically with an enzyme that produces hydrogen peroxide. Clinical Chemistry, 28, 2077 – 2080.
Friedewald, W. T., Levy, R. I. and Fredrickson, D. S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of ultracentrifuge. Clinical Chemistry, 18, 499–502.
Ganesan, K. and Xu, B. (2019). Anti-diabetic effects and mechanisms of dietary polysaccharides. Molecules, 24, 1 - 28. doi: 10.3390/molecules24142556.
Hasan, H. T. and Kadhim, E. J. (2018). Phytochemical investigation of Corchorus olitorius L. leaves cultivated in Iraq and it’s in vitro antiviral activity. Iraqi Journal of Pharmaceutical Sciences, 27, 115-122.
Hefnaway, H.T. and Ramadan, M. F. (2013). Protective effects of Lactuca sativa ethanolic extract on carbon tetrachloride induced oxidative damage in rats. Asian Pacific Journal of Tropical Disease, 3, 277-285.
Lu, Y. and Foo, Y. L. (2000). Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chemistry, 68, 81–85.
Khorrami, S., Zarrabi, A., Khaleghi, M., Danael, M., Mozafari, M. R. (2018). Selective cytotoxicity of green synthesized silver nanoparticles against MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. International Journal of Nanomedicine, 13, 8013 – 8024. http://dx.doi.org/10.2147/IJN.S189295
Meena, P. R. H., Singh, A. P., & Tejavath, K. K. (2020). Biosynthesis of Silver Nanoparticles Using Cucumis prophetarum Aqueous Leaf Extract and Their Antibacterial and Antiproliferative Activity Against Cancer Cell Lines. ACS Omega, 5, 5520–5528. https://doi.org/10.1021/acsomega.0c00155
Mohanraj, V. J. and Chen, Y. (2006). Nanoparticles – A Review. Tropical Journal of Pharmaceutical Research, 5 (1), 561-573. doi:4314/tjpr.v5i1.14634
Mohammed, A., Luka, C. D., Ngwen, A. L., Omale, O. F. R. and Yaknan, B. J. (2019). Evaluation of the effect of aqueous leaf extract of jute mallow corchorus olitorius on some biochemical parameters in alloxan-induced diabetic rats. European Journal of Pharmaceutical and Medical Research, 6 (10), 652–658.
Mu, L. and Feng, S. S. (2003). A novel controlled release formulation for the anticancer drug paclitaxel (Taxol(R)): PLGA nanoparticles containing vitamin E TPGS. Journal of Control Release, 86, 33-48.
Muhammad, S. and Shinkafi , M. A. (2014). Ethno-botanical survey of some medicinal important leafy vegetables in North Western Nigeria. Journal of Medical Plants Research, 8, 6–8.
Naito, H. K. (1984). High-density lipoprotein (HDL) cholesterol. Kaplan A . Clinical Chemistry. The C. V. Mosby Co St. Louis, Toronto Princeton. 437 and 1207-1213.
Nath, R., Roy, S., De, B. and Duttachoudhury, M. (2013). Anticancer and antioxidant activity of Croton: a review. International Journal Pharmacy and Pharmaceutical Sciences, 5, 63-70.
Neffati, N. Z., Aloui, H., Karoui, I., Guizani, M., B. and Zaouali, Y. (2017). Phytochemical composition and antioxidant activity of medicinal plants collected from the Tunisian flora. Natural Product Research, 31 (13), 1583–1588.
Nwodo, S. C., Okwuchukwu, K. E. and Solomon, O. R. (2013). Effects of Solanum aethiopicum Fruit on Plasma Lipid Profile in rats. Advances in Bioresearch, 4 (4), 79-84.
Oboh, G., Raddatz, H. and Henle, T. (2009). Characterization of the antioxidant properties of hydrophilic and lipophilic extracts of Jute (Corchorus olitorius) leaf. International of Journal Food Science and Nutrition. 60 (2), 124-134.
Ogbonna, C. C., Iwuji, S. C., Ndubuka, G. I. N., Azeez, T. O., & Okafor, S. A. (2023). In-Vivo Acute Toxicity Test of Bioactive Phytochemicals In locally used Mango and Soursop Leaves using Laboratory Mice. Engineering and Technology Journal, 08(01), 1814–1820. https://doi.org/10.47191/etj/v8i1.05
Ogunka-Nnoka, C. U., Onyegeme-Okerenta, B. M. and Omeje, H. C. (2018). Effects of Ethanol Extracts of S. aethiopicum Stalks on Lipid Profile and Haematological Parameters of Wistar Albino Rats international. Journal of Science and Research Methodology, 10(3), 215-229.
Parvin S, M. Marzan’s, S. Rahman, A. Kumer Das, S. Haque, and M. Rahmatullah, (2015) “Preliminary phytochemical screening,antihyperglycemic, analgesic and toxicity studies on methanolic extract of aerial parts of Corchorus olitorius L. Journal of Applied Pharmaceutical Science, 5 (9), 68–71.
Puskas, L.G., Nagy, Z. B. and Girics, Z. (2004). Cholesterol diet-induced hyperlipidaemia influences gene expression pattern of rat hearts: a DNA microarray study. Federation of European Biochemical Society Letter, 562, 99–104.
Ramadan, M. F., Asker, M. M. and Ibrahim, Z. K. (2008). Functional bioactive compounds and biological activities of Spirulina platensis lipids. Czechoslovakia Journal of Food Science, 26, 211-222.
Sadat, A., Hore, M., Chakraborty, K. and Roy, S. (2017). Phytochemical analysis and antioxidant activity of methanolic extract of leaves of corchorus olitorius. International Journal of Current Pharmaceutical Research, 9(5), 59–63.
Saravanan, G. and Ponmurugan, P. (2013). S-allylcysteine improves streptozotocin- induced alterations of blood glucose, liver cytochrome P450 2E1, plasma antioxidant system, and adipocytes hormones in diabetic rats. International Journal of Endocrinology Metabolism. 11(4), 1 -8. Doi:10.5812/ijem.10927
Tiong, S. H, Looi, C. and Hazni, H. (2013). Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules, 18 (8), 9770–9784.
Vila, A., Sanchez, A., Tobio, M., Calvo, P. and Alonso, M. J. (2002). Design of biodegradable particles for protein delivery. Journal of Control Release, 78, 15-24.
Xu, L., Wang, Y., Huang, J., Chen, C., Wang, Z. and Xie, H. (2020). Silver nanoparticles : Synthesis , medical applications and biosafety. Theranostics 10 (20), 8996 – 9031. https://doi.org/10.7150/thno.45413.
Zeka, K., Ruparelia, K., Arroo, R. R. J., Budriesi, R. andMicucci, M. (2017). Flavonoids and Their Metabolites : Prevention in Cardiovascular Diseases and Diabetes. Diseases, 5, 1–18. https://doi.org/10.3390/diseases5030019
Kazeem, M. I., Oyedapo, B. F., Raimi, O. G. and Adu, O. B. (2013). evalution of ficus exasperata vahl. leaf in the Management of Diabetes Mellitus in invitro. Journal of Medical Science, 13(4), 269–275. https://doi.org/10.3923/jms.2013.269.275
Wu, J., & Yan, L.-J. (2015). Streptozotocin-induced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic β cell glucotoxicity. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 8, 181–188. https://doi.org/https://dx.doi.org/10.2147/DMSO.S82272
Lucchesi, A. N., Cassettari, L. L., & Spadella, C. T. (2015). Alloxan-Induced Diabetes Causes Morphological and Ultrastructural Changes in Rat Liver that Resemble the Natural History of Chronic Fatty Liver Disease in Humans. Journal of Diabetes Research, 2015, 1–11. https://doi.org/http://dx.doi.org/10.1155/2015/494578
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