Comparative Neuroprotective Effect of Celosia argentea Linn. and Vitamin E on Mercury-induced Oxidative and Histological Parameters of Rat Brain
Keywords:
Neuroprotection, Celosia argentea, Oxidative stress, Mercuric chloride, Cerebrum, Purkinje neuronAbstract
Mercury contamination of our environment in Nigeria is increasing as mining activity increases. Its exposure causes human toxicological effects which include neurotoxicity through reactive oxygen species. This study investigated the ameliorative effects of the flavonoid-rich aqueous extract of Celosia argentea (AECA) and vitamin E (VitE) in the brain of rats treated with mercuric chloride (HgCl2). Twenty-five adult male Wistar rats were randomized into five treatment groups (n=5). Group 1- control; Group 2- HgCl2 (4 mg/kg); Group 3- AECA (400 mg/kg); Group 4- HgCl2 (4 mg/kg) + AECA (400 mg/kg); Group 5- HgCl2 (4 mg/kg) + VitE (500 mg/kg). All items were administered using an oral cannula daily for 14 days. Behavioural studies were carried out on the 16th day of experiment after which rats were euthanized. Thereafter, gross, haematological and biochemical parameters [malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT)] were assessed. Mercuric chloride significantly (p<0.05) reduced body weight of rats, SOD activity and GSH level but increased MDA level, CAT activity and the number of degenerated neurons in the cerebral cortex relative to control group. Microscopically, HgCl2 induced degeneration of cerebral cortical neurons and Purkinje neurons of the cerebellum. Treatment of HgCl2 and AECA and VitE caused a reversal of these HgCl2-induced alterations. The behavioural and haematological parameters were not significantly affected through the groups. The results suggest Celosia argentea Linn and vitamin E protected against mercury-induced gross, oxidative, cerebral and cerebellar damage. Both AECA and Vitamin E demonstrated neuroprotection in this experiment
References
Abdel Moneim, A.E. (2015). Mercury-induced neurotoxicity and neuroprotective effects of berberine Neural Regen Res. 10(6): 881–882.
Afifi, A.K. and Bergman, R.A. (2005). Functional neuroanatomy: text and atlas, 2nd edition, McGraw–Hill, New York. 201–222.
Aladesanwa, R.D., Adenawoola, A.R. and Olowolafe O.G. (2001). Effects of atrazine residue on the growth and development of celosia (Celosia argentea) under screen house conditions in Nigeria. Crop Protection. 20: 321-324.
Ansar, S. (2015). Pretreatment with diallylsulphide modulates mercury-induced neurotoxicity in male rats. Acta Biochimika Polonica. 62(3): 599–603.
Bernhoft, R.A. (2012). Mercury toxicity and treatment: a review of the literature. Journal of Environmental and Public Health. 2012: Article ID 460508, 10 pages.
Beutler, E., Duron, O. and Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J Lab Clin Med 61: 882-8.
Chaudhary, G., Sinha, K. and Gupta, Y.K. (2003). Protective effect of exogenous administration of alpha-tocopherol in liver artery occlusion model of liver ischemia in rats. Fundam Clin Pharmacol. 17: 703-7.
Clairborne, A. (1985). Catalase activity. In: Handbook of methods for oxygen radical research (R. A. Greenwald, Ed). Boca Raton, FL. pp. 283-284.
Clarkson, T.W. and Magos, L. (2006). The toxicology of mercury and its chemical compounds. Critical Reviews in Toxicology. 36(8): 609–662.
Cerecetto H, Lopez GY. 2007. Antioxidants derived from Vitamin E: An Overview. Mini-Reviews in Medicinal Chemistry, 7: 315-338.
De Zeeuw, C.I. and Hoogland, T.M. (2015). Reappraisal of Bergmann glial cells as modulators of cerebellar circuit function. Front. Cell. Neurosci. 9:246. doi: 10.3389/fncel.2015.00246.
Ebokaiwe, A.P., Adedara, I.A., Owoeye, O. and Farombi, E.O. (2013). Neurotoxicity of Nigerian bonny light crude oil in
rats. Drug and Chem Toxicol. 36(2): 187-195.
Elias, A. and Nelson, B. (2012). Toxicological effect of ciprofloxacin on testicular function of male Guinea pigs, Asian Jour. Bio Sci. 3(2): 384-390.
Ferraro, L., Tomasini, M.C., Tanganelli, S., Mazza, R., Coluccia, A., Carratu, M.R., Gaetani, S., Cuomo, V. and Antonelli, T. (2009). Developmental exposure to Methylmercury elicits early cell death in the cerebral cortex and long-term memory deficits in the rat. Int J Dev Neurosci. 27:165–174.
Goldman, L.R. and Shannon, M.W. (2001). Committee on Environmental Health Technical report: Mercury in the environment: Implications for pediatricians. American Academy of Pediatrics.108, 197–205.
Hussain, S., Rodgers, D.A., Duhart, H.M. and Ali, S.F. (1997). Mercuric chloride-induced reactive oxygen species and its effect on antioxidant enzymes in different regions of rat brain. J Environ Sci Health B. 395-409.
Ibegbu, A.O., Animoku, A., Ayuba, M., Brosu, D., Adamu, S.A., Akpulu, P., Hamman, W.O., Umana, U.E. and Musa SA. (2014). Histomorphological effect of ascorbic acid on mercury chloride- induced changes on the cerebellum of adult Wistar rats. J. Morphol. Sci. 31(4): 219-224.
Malomo, S.O., Ore, A. and Yakubu M.T. (2011). Invitro and invivo antioxidant activities of the aqueous extract of Celosia argentea var cristata leaves: Indian J Pharmacol. 43(3):278-285.
Misra, H.P. and Fridovich, I. (1972). The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry. 247: 3170-75.
Olopade, F.E., Shokunbi, M.T. and Siren, A. (2012). The relationship between the ventricular dilatation, neuropathological and neurobehavioural changes in hydrocephalic rats. Fluids and Barriers of the CNS. 9:19.
Owoeye, O., Adesida, A., Onwuka, S.K. and Farombi, E.O. (2010). Gamma radiation effects on the brain of rats: antioxidant and radioprotective properties of Vernonia amygdalina leaf extract. Int J Biol Chem Sci . 4(6): 2324-2336.
Owoeye, O., Farombi, E.O. and Onwuka, S. K. (2010). Cerebellar reduction in rats by gamma irradiation is mitigated by pretreatment with methanolic extract of Vernonia amygdalina and alpha-tocopherol. European Journal of Anatomy. 14(2): 49-58.
Owoeye, O. and Farombi, E.O. (2015). Tomato pomace protects against mercuric chloride-induced neurodegeneration and motor abnormality in adult rat. Int J Biol Chem Sci 9(3): 1142-1153.
Owoeye, O. and Onwuka, S. K. (2016). Lead Toxicity: Effect of Launaea taraxacifolia on the histological and oxidative alterations in Rat Regio III Cornu ammonis and cerebellum. Anat J Africa. 5(1): 783-794
Owoeye, O. and Arinola, G.O. (2017). A vegetable, Launaea taraxacifolia mitigated mercuric chloride alteration of the microanatomy of rat brain. J. Dietary Supplements. 14(6): 613- 625.
Ramesh, B.N., Mahalakshmi, A.M., Seema, M. and Krisha K.L. (2014). Pharmacology of Celosia argentea L. Int J Atoms and Molecules. 4(1): 635-644.
Rossi, A., Serraino, I., Dugo, P., Di Paola, R., Mondello, L., Genovese, T., Morabito, D., Dugo, G., Sautebin, L., Caputi, A.P. and Cuzzocrea, S. (2003). Protective effects of anthocyanins from blackberry in a rat model of acute lung inflammation. Free Radic Res.
: 891-900.
Rukhsana, A.R., Manohar, J.P., Priyanka, G. and Areej, S. (2013). Evaluation of antioxidant potential of Celosia argentea extracts. Pharmacognosy Journal.1–2.
Sheikh, T.J., Patel, B.J., Joshi, D.V., Patel, R.B. and Jegoda, M.D. (2013). Repeated dose oral toxicity of inorganic mercury in wistar rats: biochemical and morphological alterations, Vet World. 6(8):563-567, doi:10.5455/vetworld. 563-567
Smith, J.C., Allen, P.V., Turner, M.D., Most, B., Fisher, H.L. and Hall, L.L. (1994).The kinetics of intravenously administered methylmercury in man. Toxicol. Appl. Pharmacol. 128, 251–256.
Snell, R.S. (2006). Clinical Neuroanatomy, 6th edition. Lippincott Williams and Wilkins. pp. 219-305.
Tranel, D. (1995). Higher brain functions. In: Neuroscience in medicine. Conn PM (Ed). JB Lippincott Company, Philadelphia. pp. 555-582
Ulatowski, L., Parker, R. and Manor, D. (2014). Vitamin E is essential for Purkinje neuron integrity. Neuroscience 260: 120-129.
Uma, C., Poornima, K., Surya, S., Ravikumar, G. and Gopalakrishnan, V.K. (2012). Nephroprotective effect of ethanolic extract of Tabernaemontana coronaria in mercuric chloride induced renal damage in Wistar albino rats. J Environ Pub Health. 2012. Article ID 460508, 10 pages. doi:10.1155/2012/460508
Varshney, R. and Kale, R.K. (1990). Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. Int J Radiat Biol. 58 (5): 733-43.
Vekaria, R.H., Patel, M.N., Bhalodiya, P.N., Patel, V., Desai, T.R. and Tirgar, P.R. (2012). Evaluation of neuroprotective effect of Coriandrum sativum Linn. against ischemic-reperfusion insult in brain. Int J Phytopharmacol. 3(2):186-193.
Verma, H. and Demla, M. (2012). Standardization of Whole Plant of Celosia argentea Linn. Int J Pharmaceut Sci Res. 3(8): 2695-2699.
Vijayaprakash, S., Langeswaran, K., Gowtham, Kumar, S, Revathy, R. and Balasubramanian, M.P. (2013). Nephro-protective significance of kaempferol on mercuric chloride induced toxicity in Wistar albino rats. Biomedicine & Aging Pathology. 3: 119–124.
Xu, F., Farkas, S., Kortbeck, S., Zhang, F., Chen, L., Zamponi, G.W. and Syed, N.I., (2012). Mercury-induced toxicity of rat cortical neurons is mediated through N-Methyl-D-Aspartate Receptors. Mol Brain. 5:30.
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