Hippocampal Astrogliotic Reduction in Scopolamine Hydrobromide-Induced Alzheimer’s Type Cognitive Dysfunction Wistar Rats Following Administration of Aqueous Extract of Telfairia Occidentalis (pumpkin) seeds.
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How to Cite

Mba, E. (2021). Hippocampal Astrogliotic Reduction in Scopolamine Hydrobromide-Induced Alzheimer’s Type Cognitive Dysfunction Wistar Rats Following Administration of Aqueous Extract of Telfairia Occidentalis (pumpkin) seeds. Nigerian Journal of Physiological Sciences, 36(2), 241–244. https://doi.org/10.54548/njps.v36i2.14

Abstract

Astrocytes are small star-shaped glial cells that maintain normal human brain physiology including secretion of several active compounds and the formation of blood brain barrier. Reactive astrocytes support regenerating axons and also, actuate some genes responsible for the induction of synapse formation. In this study, the effect of aqueous extract of Telfairia occidentalis seeds on hippocampal astrogliosis was probed using scopolamine-induced Alzheimer’s type cognitive dysfunction Wistar rats. Thirty Wistar rats weighed between 180-200g were randomly grouped into five designated A, B, C, D and E.  Each group contained six rats. Alzheimer’s type cognitive dysfunction was induced in groups B to E by administering intraperitoneally, 1 mg/kg body weight of scopolamine for seven days before Donepezil and the aqueous extract of Telfairia occidentalis seeds for fourteen days. Twenty-four hours after the last administration, the animals were sacrificed; their brain tissues perfused and stained with glial fibrillary acidic protein (GFAP) dye. Results revealed prominently stained astrocytes with their processes intact (group A). Some densely stained numerous astrogliosis with hypertrophied fibres were noticed in group B. Group C demonstrated prominent astrocytes with hypertrophied fibres, group D, moderately stained astrogliosis with hypertrophied fibres while group E showed numerous astrocytes with prominent nuclei and hypertrophied fibres. In conclusion, there was reduced hippocampal astrogliosis mostly in group D treated with Telfairia occidentalis which may neutralize oxidative stress and enhanced learning and memory in the Wistar rats of the present study.

https://doi.org/10.54548/njps.v36i2.14
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References

Abbas, A. and Nelson, F. K (2004). Robbins and Cotran pathologic basis of diseases (7th ed.). New Delhi, Elsevier. P 244.

Akter, S. F. U., Rani, M. F. A., Nordin, M. S., Ab-Rahman, J., Aris, M. A. B. and Rathor, M. Y. (2012). Dementia: prevalence and risk factors. International Review of Social Sciences and Humanities, 2(2),176-184.

Alzheimer Association (2017). Alzheimer disease facts and figures. Alzheimers Dement. 4 (2), 325-373.

Anderson, L., Oldridge, N., Thompson, D. R., Zwisler, A. D., Rees, K., Martin, N. and Taylor, R. S. (2016). Exercise-based cardiac rehabilitation for coronary heart disease: Cochrane systematic review and meta-analysis. Journal of American College of Cardiology.67 (1),1-12.

Apostolova, L. G., Green, A. E., Babakchanian, S., Hwang, K. S., Chou, Y. Y., Toga, A. W., and Thompson, P. M. (2012). Hippocampal atrophy and ventricular enlargement in normal aging, mild cognitive impairment and Alzheimer’s disease. Alzheimer Disease and Associated Disorders, 26 (1), 17-27.

Eru ME, Samson OP, Anozeng OI, Mfon IA. (2020). Enhanced Effect of Aqueous Extract of Telfairia occidentalis Seed on the Microstructure of the Hippocampus of Scopolamine Hydrobromide-Induced Cognitive Dysfunction Rats, Asian J Res Rep Neu, 3(1), 5-10.

Eru ME, Samson OP, Gabriel UU, Kelechi CU, Michael EO, Udofia IE, et al. (2020). Neurobehavioural Enhancement in Scopolamine Hydrobromide-Induced Alzheimer Type Cognitive Dysfunction in Rats Following Administration of Ethanol Seed Extract of Telfairia occidentalis (Hook.f.) Cucurbitaceae. Prod Trop J Nat Res, 4(7), 282-285.

Eru M. E., Gabriel U. U., Samson O. P., Kelechi C. U., Michael E. O., Sadeyeng E. A., Nwosu C. B., Theresa N. and Anozeng O. I. (2020). Restorative Potentials of Aqueous Telfairia occidentalis Seeds Extract on the Hippocampal Nissl Granules and Short Term Memory in Alzheimer’s Type Cognitive Dysfunction Rats. Trop J Nat Prod Res

Eru M. E., Gabriel U. U., Samson O. P., Michael E. O., Michael U. A., Kelechi C. U., Sadeyeng E. A., Mfon I. A. and Anozeng O. I. (2020). Lipid Peroxidation and Glutathione Determination in Scopolamine Hydrobromide Induced Alzheimer’s Type Cognitive Dysfunction in Rats Following Administration of Ethanol Extract of Telfairia Occidentalis Seeds. Afr J Biomed Res

Ferri, C. P., Prince, M., Brayne, C., Brodaty, H., Fratiglioni, L., Ganguli, M., Hall, K., Hasegawa, K., Hendrie, H., Huang, Y., Jorm, A., Mathers, C., Menezes, P.R., Rimmer, E. and Scazufca, M. (2005). Global prevalence of dementia: a Delphi consensus study. The Lancet, 366(9503), 2112-2117.

Gillette, G. S., Abellan, V. K. G., Andrieu, S., Barberger, G. P., Berr, C., Bonnefoy, M., Dartigues, J. F., de Groot, L., Ferry, M., Galan, P, Herberg, S., Jeandel, C., Morris, M. C., Nourhashemi, F., Payette, H., Poulain, J.P., Portet, F., Roussel, A.M., Ritz, P., Rolland, Y. and Vellas, B. (2007). IANA task force on nutrition and cognitive decline with aging. Journal of Nutrition Health and Aging, 11, 132-152.

Guerchet, M., Houinato, D., Paraiso, M. N., von Ahsen, N., Nubukpo, P., Otto, M., Clement, J. P., Preux, P. M. and Dartigues, J. F. (2009). Cognitive impairment and dementia in elderly people living in rural Benin, West Africa. Dementia and Geriatric Cognitive Disorders, 27(1), 34-41.

Halassa, M. M. and Haydon, P. G (2010). Integrated brain circuits: astrocytic networks modulate neuronal activity and behaviour. Annual Review of Physiology, 72, 335-355.

Hendrie, H. C., Osuntokun, B. O., Hall, K. S., Ogunniyi, A. O., Hui, S. L., Unverzagt, F. W., Gureje, O., Rodenberg, C. A., Balyewu, O. and Musick, B. S. (1995). Prevalence of Alzheimer’s disease and dementia in two communities: Nigerian Africans and African Americans. The American Journal of Psychiatry, 152(10), 1485-1492.

Liauw, J., Hoang, S., Choi, M., Eroglu, C., Choi, M., Sun, G. H., Percy, M., Wildman-Tobriner, B., Bliss, T. and Guzman, R. G. (2008). Thrombospondins 1 and 2 are necessary for synaptic plasticity and functional recovery after stroke. Journal of Cerebral Blood Flow Metabolism, 28,1722-1732.

Kato, S., Gondo, T., Hoshi, Y., Yakahashi, M. and Yamada, M. (1998). Confocal observation of snile plaques in Alzheimer’s disease, senile plaque morphology and relationship between senile plaques and astrocytes. Pathology International, 48, 332-340.

Lee, C. H., Herman, T., Clandinin, T. R., Lee, R., and Zipursky, S. L. (2001). N-cadherin regulates target specificity in the Drosophila visual system. Neuron 30 (2), 437-450.

Liddelow, S. A., Guttenplan, K. A., Clarke, L. E., Bennett, F. C., Bohlen, C. J., Schirmer, L., Bennett, M. L., Munch, A. E., Chung, W. S. and Peterson, T. C. (2017). Neurotoxic reactive astrocytes are induced by activated microglia. Nature, 541, 481-487.

Lin, M. T. and Beal, M. F. (2006). Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature, 443, 787-795.

McGleenon, B. M., Dynan, K. B., and Passmore, A. P. (1999). Acetylcholinesterase inhibitors in Alzheimer’s disease. British Journal of Clinical Pharmacology, 48(4), 471-480.

Middeldop, J. and Hol, E. M (2011). GFAP in health and disease. Progress in Neurobiology, 93 (3), 421-443.

Nkosi, C. Z., Okpoku, A. R. and Terbalanche, S. E. (2006). Antioxidative effects of pumpkin seed (Cucurbita pepo) protein isolate in CCl4-Induced liver injury in low-protein fed rats. Phytotherapy Research. 20 (11), 935-940.

Oboha, G., Ademilayia, A. O., Akinyemia, A. J., Henleb, T., Jamiyu, A., Saliua, J. A. and Uwe Schwarzenbolzb, U (2012). Inhibitory effects of polyphenol-rich extracts of jute leaf (corchorusolitorius) on key enzyme linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin 1 converting) in vitro. Journal of Function and food. 4, 456-458.

Paddick, S., Longdon, A. R., Kisoli, A., Dotchin, C., Gray, W. K., Dewhurst, F., Chaote, P., Kalaria, R., Jusabani, A. M. & Walker, R. (2013). Dementia prevalence estimates in sub-Saharan Africa: comparison of two diagnostic criteria. Global Health Action. 6, 1–7.

Paraïso, M. N., Guerchet, M., Saizonou, J., Cowppli-Bony, P., Mouanga, A. M., Nubukpo, P., Preux, P. M. &Houinato, D. S. (2011). Prevalence of dementia among elderly people living in Cotonou, an urban area of Benin (West Africa),” Neuroepidemiology. 36 (4), 245–251.

Pharm-Huy, L. A., He, H. & Phar-Huy, C. (2008). Free radical. Antioxidants in disease and health. International Journal of Biomedical Sciences. 4 (2), 89-96.

Prince, M., Bryce, R., Albanese, E. Wimo, A., Ribeiro, W. &Ferri, C. P. (2013). The global prevalence of dementia: a systematic review and metaanalysis. Alzheimer’s and Dementia. 9(1), 63–75.

Prince, M., Comas-Herrera, A., Knapp, M., Guerchet, M. &Karagiannidom, M (2016). World Alzheimer Report 2016 improving Healthcare for people living with demetia. Coverage, quality and cost now and in the future.

Rego, C. & Oliveira, C. R. (2003). Mitochondrial dysfunction and reactive oxygen species in excitotoxicity and apoptosis: implications for the pathogenesis of neurodegenerative diseases. Neurochemical Research. 28, 1563-1574.

Silver, J. & Miller, J. H. (2004). Regeneration beyond the glial scar. Nature Reviews Neuroscience. 5, 146-156.

Simpson, J. E., Ince, P. G., Lace, G., Forster, G. & Shaw, P. J. (2008). Astrocyte phenotype in relation to Azheimer-type pathology in the ageing brain. Neurobiology of Aging, 31, 578-590.

Sofroniew, M. V. & Vinters, H. V. (2010). Astrocytes: Biology and pathology. Acta Neuropathology. 119, 7-35.

Sugimoto, H., Yamanishi, Y., Iimura, Y. & Kawakami, Y. (2000). Donepezil hydrochloride (E2020) and other acetylcholinesterase inhibitors. Current Medical Chemistry. 7, 303-339.

Verkhrasky, A. & Butt, A. (2013). Numbers: how many glial cells are in the brain? Glial Physiology and Pathophysiology. John Wiley and Sons. Pp 93-96.

Yusuf, A. J., Baiyewu, O., Sheikh, T. L. & Shehu, A. U. (2011). Prevalence of dementia and dementia subtypes among community dwelling elderly people in northern Nigeria. InternationalPsychogeriatrics. 23 (3), 379-386.

Zamanian, J. L., Xu, L., Foo, L. C., Nouri, N., Zhou, L., Giffard, R. G. & Barres, B. A (2012). Genomic analysis of reactive astrogliosis. J. Neurosci. 32, 6391- 6410.ntioxidant and anti-inflammatory activities of selected medicinal plants containing phenolics and flavonoid compounds. Journal of Agriculture, Food and Chemistry. 59 (23), 12361-12367.

Zhang, L., Ravipati, A. S., Koyyalamudi, S. R., Jeong, S. C., Reddy, N., Smith, P. T., Barlett, J., Shanmugam, K., Munch, G. & Wu, M. J. (2011). Antioxidant and antiiflammatory activities of selected medicinal plants containing phenolic and flavonoid compouds. Journal Agriculture and Food Chemistry. 59 (23), 12361-7.

Zheng, G. Z., Zhang, D., Saraf, A.,Kolasa, T., Bhatia, P., Patel, M., Lannoye, G. S., Richardson, P., Stewart, A., Rogers, J. C., Brioni, J. D. &Surowy, C. S. (2007). Fatty acid amide hydrolase inhibitors display broad selectivity and inhibit multiple carboxylesterases as off-targets. Neuropharmacology. 4 (52), 1095-1105.

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