Effect of Chronic Caffeine Consumption on Cardiac Tissue Metabolism in the Rabbit
Avainsanat:
Caffeine, Cardiac tissue metabolism, RabbitAbstrakti
Summary: Previous studies on the ability of caffeine to enhance endurance and boost performance have focused on the energy substrates that are utilized by the skeletal muscle and the brain but nothing of such has been reported on cardiac tissue. This study was designed to investigate the effect of caffeine on cardiac tissue metabolism in the rabbit. The study was carried out on adult male New Zealand rabbits divided into 3 groups (n=5). Group I rabbits served as control and were given 0.5ml/Kg of normal saline while group II and III rabbits were administered with 2mg/Kg and 6mg/kg of caffeine respectively for 28 days. Blood samples were collected by retro orbital puncture for biochemical analysis. Animals were sacrificed by cervical dislocation and cardiac tissue biopsies were collected for biochemical and immunohistochemical analysis. Cardiac tissue glycogen concentration was determined by anthrone reagent method. Cardiac tissue CPT 1 activity and cAMP concentration were determined by immunohistochemistry and colorimetry techniques respectively, with assay kits obtained from Biovision Inc. The results showed that Caffeine at 2 and 6 mg/kg significantly inhibited MPO activity from 0.72±0.05 to 0.164±0.045 and 0.46±0.12 U/L respectively (p<0.05). Caffeine at 2mg/kg had no effect on serum nitric oxide but at 6mg/Kg, it significantly increased serum nitric oxide form 28.01±6.53 to 45.25±3.88μM of nitrite (p<0.05). Also, Caffeine at 2 and 6mg/kg increased cardiac tissue glycogen from 15.62±0.73 to 40.69±6.35 and 38.82±6.91mg/100g respectively and carnitine palmytol transferase 1 activity from 18.3 to 20 and 25.2% respectively. In conclusion, the study showed that caffeine consumption increased CPT 1 activity suggesting increased utilization of free fatty acids for energy metabolism and sparing of cardiac tissue glycogen by mechanism(s) which probably involved blockade of A1 adenosine receptors and cAMP signaling pathway.
Lähdeviitteet
Berg, J. M., Tymoczo, J. L. and Stryer, L. 2007. Biochemistry 6th edition.
Chaea, S.Y. et al. (2004) Protection of insulin secreting cells from nitric oxide induced cellular damage by crosslinked hemoglobin. Biomaterials25, 843ñ50.
Chavez-Valdez, R., Wills-Karp, M., Ahlawat, R., Cristofalo, E. A. Nathan, A. and Gauda, E. B. 2009. Caffeine modulates TNF-α production by cord blood monocytes: the role of adenosine receptors. Pediatrics Research 65: 203–208.
Christopherson KS, Bredt DS. 1997 Nitric oxide in excitable tissues: physiological roles and disease. Journal of Clinical Investigation 1997 100: 2424-2429.
Chukwu, L. O., Odiete, W. O. and Briggs, L. S. 2006, Basal metabolic regulatory responses and rhythmic activity of mammalian heart to aqueous kolanut extracts. African Journal of Biotechnology 5:484-486.
Dekker, M. J., Gusba, J. E., Robinson L. E. and Graham T. E. 2007. Glucose homeostasis remains altered by acute caffeine ingestion following 2 weeks of daily caffeine consumption in previously noncaffeine consuming males. British Journal of Nutrition 98:556 562
Egawa, T., Hamada. T., Kameda, N., Karaike, K., Ma, X., Masuda, S., Iwanaka, N. and Hayashi, T. 2009. Caffeine acutely activates 5_adenosine monophosphate-activated protein kinase and increases insulin-independent glucose transport in rat skeletal muscles. Metabolism 58, 1609–1617.
Goldstein ER, Ziegenfuss T, Kalman D, Kreider R, Campbell B, Wilborn C, Taylor L, Willoughby D, Stout J, Graves BS, Wildman R, Ivy JL, Spano M, Smith AE & Antonio J (2010). International society of sports nutrition position stand: caffeine and performance. J Int Soc Sports Nutr7,5.
Graham T. E., Spriet L. L. 1991: Performance and metabolic responses to a high caffeine dose during prolonged endurance exercise. Journal of Applied Physiology 71:2292-98.
Graham TE, Spriet LL. 1991 Performance and metabolic responses to a high caffeine dose during prolonged exercise. J Appl Physiol. 71:2292–229.
Graham, T. E. Sathasivam, P., Rowland, M., Marko, N., Greer, F. and Battram, D. 2001. Caffeine ingestion elevates plasma insulin response in humans during an oral glucose tolerance test. Canadian Journal of Physiology and Pharmacology 79: 559–565.
Isehunwa, G. O. Olaniyan, O. T. and Alada, A. R. A. The role of alpha and beta adrenergic receptors in cortisol-induced hyperglycaemia in the common African toad (Bufo regularis). African Journal of Biotechnology. Vol. 12(36), pp. 5554-5558, 4 September, 2013.
Jensen, T. E., Rose, A. J., Hellsten, Y., Wojtaszewski, J. F. and Richter, E. A. 2007. Caffeine-induced Ca2+ release increases AMPK-dependent glucose uptake in rodent soleus muscle. American Journal of Physiology- Endocrinology and Metabolism 293: E286–E292.
Kempf, K., Herder, C., Erlund, I., Kolb, H., Martin, S., Carstensen, M., Koenig, W., Sundvall, J., Bidel, S., Kuha, S. and Tuomilehto S. 2010. Effects of coffee consumption on subclinical inflammation and other risk factors for type 2 diabetes: a clinical trial. J. American Journal of Clinical Nutrition 4:950-7. Leblanc, J., Richard, D. and Racotta, I. S. 1995. Metabolic and hormone-related responses to caffeine in rats. Pharmacological Research 32.3:129-33.
Lopaschuk GD, Belke DD, Gamble J, et al.: Regulation of fatty acid oxidation in the mammalian heart in health and disease. Biochim Biophys Acta 1994, 1213:263–276. Lv, X., Chen, Z., Li, J., Zhang, L., Liu, H., Huang, C, and Zhu, P. 2010. Caffeine protects against alcoholic liver injury by attenuating inflammatory response and oxidative stress. Inflammation Research 59: 635–645.
McGarry, J. D., Mannaerts, G. P., and Foster, D. W. 1977. A possible role for malonyl-CoA in the regulation of hepatic fatty acid oxidation and ketogenesis. Journal of Clinical Investigation 60: 265–270.
McGarry, J. D., Mannaerts, G. P., Foster. and D. W. 1978. Characteristics of fatty acid oxidation in rat liver homogenates and the inhibitory effect of malonyl-CoA. Biochimica et Biophysica Acta 530:305–313.
Milanez, S. 2011. Adverse health effects of caffeine: Review and analysis of recent human and animal research. U.S. Food and Drug Administration. College Park MD: 20740-3835
Muroyama K, Murosaki S, Yamamoto Y, Odaka H, Chung HC, Miyoshi M. 2003 Anti-obesity effects of a mixture of thiamin, arginine,caffeine, and citric acid in non-insulin dependent diabetic KK mice. Journal of Nutritional Science and Vitaminology 49:56–63.
Nagasawa, H., Nakajima, T., Yamamura, M., Kaku, S. and Hongou, N. 2001. Effects of chronic treatment with caffeine on behaviour and related parameters in male and female mice. In vivo 15.6: 495-501.
Nehlig, A. 1999. Are we dependent upon coffee and caffeine? A review on human and animal data. Neuroscience and Biobehavioral Reviews 23.4: 563–576.
Nehlig, A., Daval, J., Pereira, L. and Boyet, V. A., 1987. Caffeine-diazepam interaction and local cerebral glucose utilization in the conscious rat. Brain Research Reviews 419:272–278.
Pencek, R. R., Battram, D., Shearer, J., James, F.D., Lacy, D. B., Jabbour, K., Williams, P. E., Graham, T. E. and Wasserman, D. H. 2004. Portal vein caffeine infusion enhances net hepatic glucose uptake during a glucose load in conscious dogs. Journal of Nutrition 134: 3042–3046.
Pizziol, A., Tikhonoff, V., Paleari, C.D., Russo, E., Mazza, A., Ginocchio, G., Onesto, C., Pavan, L., Casiglia, E. and Pessina A.C. 1998. Effects of caffeine on glucose tolerance: a placebo-controlled study. European Journal of Clinical Nutrition 52: 846-849. Ray, K. 2013 Liver: caffeine is a potent stimulator of autophagy to reduce hepatic lipid content–a coffee for NAFLD? Nature Reviews Gastroenterology and Hepatology 10: 563.
Saddik M, Lopaschuk GD: Myocardial triglyceride turnover and contribution to energy substrate utilization in isolated working rat hearts. J Biol Chem 1991, 266:8162–8170.
Salahdeen, H.M. and Alada, A.R. 2009. Effect of caffeine and ethanolic extract of kolanut on glucose uptake in the canine hindlimb at rest and during contraction. Nigerian Journal of Physioliogical Sciences. 24.1: 33-45.
Schindhelm, R.K., Van der Zwan, L.P., Teerlink, T. and Scheffer, P.G. 2009. Myeloperoxidase: A Useful Biomarker for Cardiovascular Disease Risk Stratification? Clinical Chemistry 55: 81462–1470.
Stanley WC, Chandler MP: Energy metabolism in the normal and failing heart: potential for therapeutic interventions. Heart Fail Rev 2002, 7:115–130.
Stiles G.L Adenosine receptors: Structure, Function and Regulation. TIPS Review pages 486 – 490.
Taegtmeyer, H. 1994. Energy metabolism of the heart: from basic concepts to clinical applications. Current Problems in Cardiology. 19: 59-113.
Xia Y, Zweier J. L. 1997. Measurement of myeloperoxidase in leukocyte-containing tissues. Anal Biochem 245:93–96.
Yang, J.N., Chen, J.F. and Fredholm, B.B. 2009. Physiological roles of A1 and A2A adenosine receptors in regulating heart rate, body temperature,
and locomotion as revealed using knockout mice and caffeine. American Journal of Physiology. Heart Circulatory Physiology. 296: H1141–H1149.
Yokogoshi, H., Mochizuki, S., Takahata, M., Quazi, S. and Yoshida A. 1983 Nutrition Reports International 28:805.
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