Modulatory Effects of Sesame Seed Oil on Permethrin-Induced Testicular Dysfunctions in Rats
DOI:
https://doi.org/10.54548/چکیده
Permethrin (PM) is a synthetic type 1 pyrethroid insecticide, and excessive exposure can cause a wide range of toxicity. This study evaluated the activities of sesame seed oil (SSO) on the testicular histology and male reproductive parameters in permethrin-induced toxicity in adult Wistar rats. Forty (40) adult male Wistar rats were randomly allocated into four (4) groups of 10 animals each. Group A received 0.5 ml of normal saline; Group B was given feed mixed with 1,000 mg/kg of 0.6% permethrin; Group C received 5 ml/kg of sesame seed oil, and Group D received feed mixed with 1,000 mg/kg of 0.6% permethrin and 5 ml/kg of sesame seed oil. All treatment was done daily for 4 weeks using an oral cannula. The animals were anesthetized and transcardially perfused with normal saline and 10% buffered formalin. The testis was processed for tissue histology, superoxide dismutase, glutathione, malondialdehyde, testosterone, luteinizing hormone, and follicle-stimulating hormone were assayed. Furthermore, sperm count, motility, morphology, and viability were evaluated using the caudal epididymis. The result showed reduced body weight and testicular weight in the permethrin (PM) group. There was a reduction in the level of semen parameters due to permethrin exposure. There was also a reduction in the level of testosterone, coupled with a high level of follicle-stimulating hormone and luteinizing hormone in the PM group. Permethrin disrupts endogenous antioxidants, and there were degenerative changes in the testicular histoarchitecture in the PM group. The sesame seed oil protected against oxidative damage in male Wistar rats. It regulated the hormonal profiles and improved the caudal epididymal sperm quality as well as improved testicular histoarchitectural integrity. This study showed that sesame seed oil improved testicular histoarchitecture and enhanced reproductive parameters in permethrin-induced testicular damage.
Key words: Permethrin, Sesame seed oil, Glutathione, Testosterone, histoarchitecture
مراجع
Abbasi, Z., Tabatabaei, S. R. F., Mazaheri, Y., Barati, F., Morovvati, H. (2013). Effects of Sesame Oil on the Reproductive Parameters of Diabetes
Abdel-Daim M, El-Bialy BE, Rahman, H. G. A, Radi, A. M. Hefny, H. A. Hassan, A. M. (2016). Antagonistic effects of Spirulina platensis against sub-acute deltamethrin toxicity in mice: biochemical and histopathological studies. Biomed Pharmacother. 77:79–85. https://doi.org/10.1016/j.biopha.2015.12.003.
Afeez, H. (2021). Food poisoning: How bacterial, chemical contamination, unsafe food handling cause children, adults’ death - Punch Newspapers. Punchng. https://punchng.com/food-poisoning-how-bacterial-chemical-con. tamination-unsafe-food-handling-cause-children-adults-death/ (accessed February, 1514, 2025).
Arumugam, P. and Ramesh, S. (2011). Protective effects of sesame oil on 4-NQO-induced oxidative DNA damage and lipid peroxidation in rats. Drug Chem Toxicol ;34:116-9
ATSDR (Agency for Toxic Substances and Disease) (2005) Registry. Toxicologic information about insecticides used for eradicating mosquitoes (West Nile virus control-permethrin).Available:http://www.atsdr.cdc.gov/consultations/west_nile_virus.
Baltazar, M. T., Dinis-Oliveira, R. J., de Lourdes Bastos, M., Tsatsakis, A. M., Duarte, J. A., Carvalho, F. (2014). Pesticides exposure as etiological factors of Parkinson’s disease and other neurodegenerative diseases – a mechanistic approach. Toxicol Lett 2014;230:85–103. doi: 10.1016/j. toxlet.01.039
Banks, S. D., Murray, N., Wilder-Smith, A., Logan, J. G. (2014). Insecticide-treated clothes for the control of vector-borne diseases: a review on effectiveness and safety. Med Vet Entomol. 28(Suppl 1):14-25.
Carloni, M., Nasuti, C., Fedeli, D. (2012) The impact of early life permethrin exposure on development of neurodegeneration in adulthood. Exp. Gerontol. 47, 60–66.
Chemineau, P., Cogine, Y., Guerin, Y., Orgeure, P., Valtet, JC. (1991). Training Manual on Artificial Insemination in sheep and goats. FAO, Trop Anim Health Prod. 77- 83.
Chen, D., Yu., S. P., Wei, L. (2014). Ion channels in regulation of neuronal regenera
Costa, L. G. (2015). The neurotoxicity of organochlorine and pyrethroid pesticides. In: Handbook of clinical neurology. 131. Ch 9. Amsterdam: Elsevier; 135–48. doi: 10.1016/B978-0-444-62627-1.00009-3
Evans, G., and Maxwell, WMC. (1987). Salamon‟s Artificial Insemination of Sheep and Goats. Butterworths Aust. tax. legis 33.
Falcioni, M. L., Nasuti, C., Bergamini, C. (2010) The primary role of glutathione against nuclear DNA damage of striatum induced by permethrin in rats. Neuroscience 168, 2–10.
Field, L. M., Emyr Davies, T. G., O’Reilly, A. O., Williamson, M. S., Wallace, B. A. (2017). Voltagegated sodium channels as targets for pyrethroid insecticides. Eur Biophys J.
Furlong, M. A, Barr, D. B., Wolff, M. S., Engel, S. M. (2017). Prenatal exposure to pyrethroid pesticides and childhood behavior and executive functioning. Neurotoxicology ;62:231–8.
Green, D. R., and Llambi, F. (2015). Cell death signaling. Cold Spring Harbor Perspectives in Biology, 7(11), a006080.
Hamadache, M., Benkortbi, O., Hanini, S., Amrane, A., Khaouane, L., Si, Moussa, C. A. (2016). Quantitative structure activity relationship for acute oral toxicity of pesticides on rats: validation, domain of application and prediction. J Hazard Mater. ;303:28–40. https://doi.org/10.1016/j.jhazmat.2015.09.021.
Hauet, T., Navratil, V., Dolo, L., Lesné, T., & Saboureau, M. (2005). Permethrin inhibits Leydig cell steroidogenesis through alteration of cholesterol transport. Toxicology and Applied Pharmacology, 208(1), 77-84.
Hauptman, D, Perić, M. H., Marić, T., Bojanac, A. K., Sinčić, N, Zimak Z, et al. (2021). Leydig Cells in Patients with Non-Obstructive Azoospermia: Do They Really Proliferate? Life Basel Switz. 11(11). doi:10.3390/life11111266.
HSDB (Hazardous Substances Data Bank. National Library of Medicine) (2003) National Toxicology Program, Bethesda, MD: HSDB. Available: http://toxnet.nlm.nih.gov/cgi-bin/sis/htmlgen?
Imam, A. L., Okesina, A. A., Sulaimon, F. A., Imam, A., Ibiyeye, R. Y., Oyewole, L. A. et al. (2024a) Thymoquinone ameliorate oxidative stress, GABAergic neuronal depletion and memory impairment through Nrf2/ARE signaling pathway in the dentate gyrus following cypermethrin administration. . BMC Neuroscience. 25:(45) 1-10. https://doi.org/10.1186/s12868-024-00896-7
Imam, A. L., Okesina, A. A., Sulaimon, F A., Ibiyeye, R
Y., Oyewopo, A. O., Biliaminu, S. A., et al., (2021). Melatonin Mitigates Sodium Flouride-induced Hormonal Imbalance, Oxidative stress, Seminal Fluid Alteration and Seminiferous Tubule Cytoarchitecture in Rats. Nigeria Society of Experimental Biology Journal. 21(2): 61-68.
Imam, A. L., Okesina, AA., Sulamon, FA., Imam, A., Ibiyeye, RY., Adana, M. Y., (2024b). Thymoquinone Ingestions Reversed Inflammation Driven Glia activation and Impaired Cognitive associated Behaviour in Cypermethrin Exposed Rats. J NeurobehavSci.11:(2)38-44.https://dergipark.org.tr/tr/download/article-file/4171398.
Jin Y., Liu J., Wang L. (2012) Permethrin exposure during puberty has the potential to enantioselectively induce reproductive toxicity in mice. Environ. Int. 42, 144–151.
Junqueira, L. C, Carneiro, J: (2005). Basic Histology- Text and Atlas 11th Edition. The McGraw-Hill Companies.
Kang, M H., Naito, M., & Matsuura, H. (2000). Antitumor-promoting effects of sesamol and its related compounds. Cancer Letters, 155(1), 41–46.
Kang, Y. H., Park, Y. K., Lee, G. D. (1998). Antioxidative properties of sesame hull extracts. Food Science and Biotechnology, 7(3), 209–213.
Keystone, J. S., Kozarsky, P. E., Connor, B. A., Wolfe, M. S., & Brunette, G. W. (2013). Health information for international travel 2014: the yellow book. Oxford University Press.
Khaneshi, M. A., Moshtaghian, J., Norouzi, M. (2013). Protective effect of sesame oil on testicular tissue in streptozotocin-induced diabetic rats. Journal of Reproduction & Infertility, 14(3), 130–137.
Kimani, E. W., Vulule, J. M., Kuria, I. W., Mugisha, F. (2006). Use of insecticide-treated clothes for personal protection against malaria: a community trial. Malar J 5:63.
Luke, J. (2001). Fluoride deposition in the aged human pineal gland. 35(2) Caries Res. 125-8.
Mohammadi, H., Ghassemi-Barghi, N., Malakshah, O., Ashari, S. (2019). Pyrethroid exposure and neurotoxicity: a mechanistic approach. Archives Industrial Hygiene Toxicol. ;70:74–89. https://doi.org/10.2478/aiht-2019-70-3263.
Mohammadzadeh, M., Pourentezari, M., Zare-Zardini, H., Nabi, A., Esmailabad, SG., Khodadadian, A., Talebi, AR. (2021). The effects of sesame oil and different doses of estradiol on testicular structure, sperm parameters, and chromatin integrity in old mice. Clin Exp Reprod Med. 48(1):34-42
Mostafa, M. G., El-Gohary, F. A., & Hussein, Y. A. (2016). Protective effect of vitamin E against permethrin-induced testicular toxicity in rats. Andrologia, 48(6), 629–638.
Nasuti, C., Fattoretti, P., Carloni, M., Fedeli, D., Ubaldi, M., Ciccocioppo, R, et al. (2014). Neonatal exposure to permethrin pesticide causes lifelong fear and spatial learning deficits and alters hippocampal morphology of synapses. J Neurodev Disord ;6:7.
Okpalanduka, C. B. and Agu, G. O. (2018). Permethrin-Induced Decrease in Libido and androgen levels in Albino Rats. Scientia Africana. 17(2): 166-176
Omotoso, G. O., Onanuga, I. O., Ibrahim, R. B. (2022). Histological Effects of Permethrin Insecticide on the Testis of Adult Wistar Rats. Ibnosina Journal of Medicine and Biomedical Sciences. 125-129
Omotoso, G. O., Onanuga, I. O., & Ibrahim, R. B. (2021). Effects of permethrin on the testes of adult wistar rats. Journal of Applied Pharmaceutical Science, 4(09), 061-065.
Orsborne, J., Deraedt Banks, S., Hendy, A., Gezan, S. A., Kaur, H., Wilder-Smith, A, et al. (2016). Personal protection of permethrin-treated clothing against Aedes aegypti, the vector of Dengue and Zika virus, in the laboratory. PLoS One. 11(5):e0152805.
Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci V et al: (2017). Oxidative stress: Harms and Benefit for Human Health. Oxid. Med Cell Longev. (3) 1-13. Doi: 10.1155/2017/8416763.
Robb, G., Amann, R., Killian, G. (1978). Daily sperm production and epididymal reserves of pubertal and adult rats. J Repro. Fertil. 54,103 - 107.
Roma, L. P., Camargo, J. L., & Martinez, C. A. (2012). Effects of permethrin on biochemical and hematological parameters of the neotropical freshwater fish Prochilodus lineatus. Ecotoxicology and Environmental Safety, 80, 262–267.
Rong, C. P.. Liong, C. K., Chen, S. T., Jen, C. C., Liu, T. L., Cheng, H. (2005). Dietary sesame reduces serum cholesterol and enhances antioxidant capacity in hypercholesterolemia. Nutr Res, 25: 559-567.
Sankar, D., Rao, MR., Sambantham, PT., & Rao, L. (2006). Antioxidant activity and protective effect of sesame oil against radiation induced lipid peroxidation. Indian Journal of Clinical Biochemistry, 21(2), 162–168.
Sheehan, D. C., Hrapchak, B. B. (1987). Theory and Practice of Histotechnology, 2nd Edition; Battelle Memorial Institute, Columbus, Ohio. 262-264.
Shittu, L. A. J., Shittu, R. K., Ogundife, O., Tayo, A. O., Osunubi, A. A. .A. (2009). Hypoglycaemia and improved testicular parameters in Sesamum radiatum treated normo-glycaemic adult male Sprague Dawley rats. Afr J Biotechnol. 8:2878-86
Soderlund, D. M. (2012). Molecular mechanisms of pyrethroid insecticide neurotoxicity: recent advances. Arch Toxicol. 86(2):165-81.
Vadhana, M. D., Carloni, M., Nasuti, C. et al. (2011) Early life permethrin insecticide treatment leads to heart damage in adult rats. Exp. Gerontol. 46, 731–738.
Vasan, S. S. (2011). Semen analysis and sperm function tests: how much to test? IJU J Urol Soc India. 27(1):41-8.
Yoon, I. S., Fenske, R. A., & Kissel, J. C. (2003). Dermal absorption of permethrin from treated clothing in simulated dislodgeable foliar residue. Journal of Exposure Analysis and Environmental Epidemiology, 13(5), 369–378.
Zhang, S-Y., Ito, Y. Y., Amanoshita, O., Yanagiba, Y., Kobayashi, M., Taya, K. et al. (2007). Permethrin may disrupt testosterone biosynthesis via mitochondrial membrane damage of Leydig cells in adult male mouse. Endocrinology. 148(8):3941-9.
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