Evaluation of Testicular Function and Structural Changes of Wistar Rats Following Antiretroviral Exposure: Protective Role of Cyperus Esculentus

Authors

  • Oluwatosin Olalekan Ogedengbe Federal University Oye Ekiti
  • A. Bature
  • A.A. Fafure
  • S.O. Kehinde
  • A.O. Adekeye
  • C.O. Akintayo
  • B.O. Ajiboye
  • O.A. Adeeyo

DOI:

https://doi.org/10.54548/njps.v38i2.9

Keywords:

Cyperus Esculentus,, highly active antiretroviral therapy,, testicular dysfunction,, antioxidant, Testosterone,, Follicle-stimulating hormone,

Abstract

Long-term antiretroviral drug toxicity may exacerbate the impact of HAART-Cyperus esculentus (C. esculentus) interactions on testicular function in HIV-infected individuals. This study examined the ability of C. esculentus plants to treat testicular dysfunction, which is thought to be a probable side effect of antiretroviral toxicity. Adult Wistar male rats weighing 90–110 g were divided into six groups and administered the prescribed treatments. In addition to testicular histology and stereological parameters, testosterone levels, follicle-stimulating hormone levels, antioxidant markers, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione levels were also evaluated. The adverse consequences of highly active antiretroviral therapy (HAART) include considerable loss of germ cells, enlargement of the tubular lumen, widening of interstitial gaps, and severe hypocellularity. Compared to the other treatment groups, MDA levels dramatically increased, whereas GSH and antioxidant enzyme (SOD) levels significantly decreased. Testicular architecture was largely conserved after treatment with C. esculentus, with a notable increase in the cellular densities of germinal and interstitial cells and a notable decrease in the tubular lumen. Vacuolation, architectural malformations, and hypoplastic changes were reduced. Significant improvements were also observed in C. esculentus in terms of elevated antioxidant SOD and GSH levels and decreased MDA levels. C. esculentus reduced architectural distortions and testicular dysfunction caused by HAART, and improved testicular morphology. Further exploration of these pathways is required.

References

ACHILA, O. O., ABRHALEY, F., KESETE, Y., TESFALDET, F., ALAZAR, F., FISSHAYE, L., GEBREMESKEL, L., MEHARI, R. & ANDEMICHAEL, D. 2022. Dyslipidemia and associated risk factors among HIV/AIDS patients on HAART in Asmara, Eritrea. PloS one, 17, e0270838.

ACHORIBO, E. S. & ONG, M. T. 2017. TIGER NUT (CYPERUS ESCULENTUS): SOURCE OF NATURAL ANTICANCER DRUG? BRIEF REVIEW OF EXISTING LITERATURE. Euromediterranean Biomedical Journal, 12.

ADANA, M., AKANG, E., PETER, A., JEGEDE, A., NAIDU, E., TILOKE, C., CHUTURGOON, A. & AZU, O. 2018. Naringenin attenuates highly active antiretroviral therapy‐induced sperm DNA fragmentations and testicular toxicity in Sprague‐Dawley rats. Andrology, 6, 166-175.

ADELAKUN, S. A., AKINTUNDE, O. W. & OGUNLADE, B. 2021. Fluoride-induced testicular degeneration and sperm quality deteriorations: Salutary role of Cyperus esculentus tubers (tiger nut) extract in animal model. Revista Internacional de Andrología, 19, 201-212.

AKHIGBE, R., HAMED, M. & AREMU, A. 2021. HAART exacerbates testicular damage and impaired spermatogenesis in anti-Koch-treated rats via dysregulation of lactate transport and glutathione content. Reproductive Toxicology, 103, 96-107.

AL-SHAIKH, M. N., WAHAB, T. A., KAREEM, S. A. & HAMOUDI, S. R. 2013. Protective effect of chufa tubers (Cyperus esculentus) on induction of sperm abnormalities in mice treated with lead acetate. Int J Drug Dev Res, 5, 99-102.

ALBRO, P. W., CORBETT, J. T. & SCHROEDER, J. L. 1986. Application of the thiobarbiturate assay to the measurement of lipid peroxidation products in microsomes. Journal of Biochemical and Biophysical Methods, 13, 185-194.

ALBUS, U. 2012. Guide for the Care and Use of Laboratory Animals (8th edn). SAGE Publications Sage UK: London, England.

ATOIGWE-OGEYEMHE, B. E., ODIGIE, E. B. & ACHUKWU, P. U. 2018. Aqueous Extract of Cyperus esculentus L.(Cyperaceae) Enhances Libido and Spermatogenesis in Male Wistar Rats: doi. org/10.26538/tjnpr/v2i11. 2. Tropical Journal of Natural Product Research (TJNPR), 2, 471-475.

BAZINE, T. & ARSLANOĞLU, F. 2020. Tiger nut (Cyperus esculentus); morphology, products, uses and health benefits. Black Sea Journal of Agriculture, 3, 324-328.

BERWAL, M. & RAM, C. 2018. Superoxide dismutase: A stable biochemical marker for abiotic stress tolerance in higher plants. Abiotic and biotic stress in plants, 1-10.

BIELLI, A., KATZ, H., PEDRANA, G., GASTEL, M. A. T., MORAÑA, A., CASTRILLEJO, A., LUNDEHEIM, N., FORSBERG, M. & RODRIGUEZ-MARTINEZ, H. 2001. Nutritional management during fetal and postnatal life, and the influence on testicular stereology and Sertoli cell numbers in Corriedale ram lambs. Small Ruminant Research, 40, 63-71.

BISHT, S., FAIQ, M., TOLAHUNASE, M. & DADA, R. 2017. Oxidative stress and male infertility. Nature Reviews Urology, 14, 470-485.

COSTA, J., BRAGA, P. C., REBELO, I., OLIVEIRA, P. F. & ALVES, M. G. 2023. Mitochondria Quality Control and Male Fertility. Biology, 12, 827.

EVANS, W. 2002. Trease and Evans Pharmacognosy. 15th edtion. Edinburgh, Saunders, 249.

HAAS, D. W. & TARR, P. E. 2015. Perspectives on pharmacogenomics of antiretroviral medications and HIV-associated comorbidities. Current Opinion in HIV and AIDS, 10, 116-122.

HARBONE, J. 1973. Methods of Plant Analysis Chapter II In: Phytochemical methods: A guide to modern techniques of plant analysis Toppan Company Ltd. Japan, 1, 4-5.

HOWARD, V. & REED, M. 2004. Unbiased stereology: three-dimensional measurement in microscopy, Garland Science.

HUFF, B. 2003. Showdown at the X4 Corral. GMHC Treatment Issues: the Gay Men's Health Crisis Newsletter of Experimental AIDS Therapies, 17, 1-5.

HUSSAIN, T., KANDEEL, M., METWALLY, E., MURTAZA, G., KALHORO, D. H., YIN, Y., TAN, B., CHUGHTAI, M. I., YASEEN, A. & AFZAL, A. 2023. Unraveling the harmful effect of oxidative stress on male fertility: A mechanistic insight. Frontiers in Endocrinology, 14.

JEDLINSKA-KRAKOWSKA, M., BOMBA, G., JAKUBOWSKI, K., ROTKIEWICZ, T., JANA, B. & PENKOWSKI, A. 2006. Impact of oxidative stress and supplementation with vitamins E and C on testes morphology in rats. Journal of Reproduction and Development, 52, 203-209.

JENSEN, T. K., ANDERSSON, A.-M., HJOLLUND, N. H. I., SCHEIKE, T., KOLSTAD, H., GIWERCMAN, A., HENRIKSEN, T. B., ERNST, E., BONDE, J. P. & OLSEN, J. 1997. Inhibin B as a serum marker of spermatogenesis: correlation to differences in sperm concentration and follicle-stimulating hormone levels. A study of 349 Danish men. The Journal of Clinical Endocrinology & Metabolism, 82, 4059-4063.

KEHINDE, S. O., OGEDENGBE, O. O., LINUS, E. A., ERINOMO, O. O., AJUMOBI, K. O., NAIDU, E. C. S. & AZU, O. O. 2021. Histomorphological study of the effects of aqueous extract of Curcuma longa on highly active antiretroviral therapy‐induced testicular toxicity. Andrologia, 53, e13952.

LENZI, A., GANDINI, L., PICARDO, M., TRAMER, F., SANDRI, G. & PANFILI, E. 2000. Lipoperoxidation damage of spermatozoa polyunsaturated fatty acids (PUFA): scavenger mechanisms and possible scavenger therapies. Frontiers in Bioscience-Landmark, 5, 1-15.

MALVIYA, N., MALVIYA, S., JAIN, S. & VYAS, S. 2016. A review of the potential of medicinal plants in the management and treatment of male sexual dysfunction. Andrologia, 48, 880-893.

MASUKU, N. P., UNUOFIN, J. O. & LEBELO, S. L. 2020. Promising role of medicinal plants in the regulation and management of male erectile dysfunction. Biomedicine & Pharmacotherapy, 130, 110555.

MOHLALA, K., OFFOR, U., MONAGENG, E., TAKALANI, N. B. & OPUWARI, C. S. 2023. Overview of the Effects of Moringa oleifera Leaf Extract on Oxidative Stress and Male Infertility: A Review. Applied Sciences, 13, 4387.

NAIDU, E. C. S., OLOJEDE, S. O., LAWAL, S. K., RENNIE, C. O. & AZU, O. O. 2021. Nanoparticle delivery system, highly active antiretroviral therapy, and testicular morphology: The role of stereology. Pharmacology Research & Perspectives, 9, e00776.

NWANGWA, J. N., UDEFA, A. L., AMAMA, E. A., INAH, I. O., IBRAHIM, H. J., IHEDURU, S. C., OKORIE, N. E., OGAR, J. A., MADAKI, F. N. & OWAI, P. O. 2020. Cyperus esculentus L.(tigernut) mitigates high salt diet‐associated testicular toxicity in Wistar rats by targeting testicular steroidogenesis, oxidative stress and inflammation. Andrologia, 52, e13780.

NWOSU, L. C., EDO, G. I. & ÖZGÖR, E. 2022. The phytochemical, proximate, pharmacological, GC-MS analysis of Cyperus esculentus (Tiger nut): A fully validated approach in health, food and nutrition. Food Bioscience, 46, 101551.

ODUWOLE, O. O., HUHTANIEMI, I. T. & MISRAHI, M. 2021. The roles of luteinizing hormone, follicle-stimulating hormone and testosterone in spermatogenesis and folliculogenesis revisited. International journal of molecular sciences, 22, 12735.

OGEDENGBE, O., JEGEDE, A., ONANUGA, I., OFFOR, U., PETER, A., AKANG, E., NAIDU, E. & AZU, O. 2018a. Adjuvant potential of virgin coconut oil extract on antiretroviral therapy‐induced testicular toxicity: An ultrastructural study. Andrologia, 50, e12930.

OGEDENGBE, O., NAIDU, E., AKANG, E., OFFOR, U., ONANUGA, I., PETER, A., JEGEDE, A. & AZU, O. 2018b. Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy. Andrology, 6, 616-626.

OGEDENGBE, O. O., NAIDU, E. & AZU, O. O. 2018c. Antiretroviral therapy and alcohol interactions: X-raying testicular and seminal parameters under the HAART era. European journal of drug metabolism and pharmacokinetics, 43, 121-135.

OGUNLADE, B., JEJE, S., ADELAKUN, S. & AKINGBADE, G. 2022. Moringa oleifera restored semen quality, hormonal profile, and testicular morphology against Highly Active Antiretroviral Therapy-induced toxicity in adult male Wistar rats. JBRA Assisted Reproduction, 26, 3.

OLABIYI, A. A., CARVALHO, F. B., BOTTARI, N. B., LOPES, T. F., DA COSTA, P., STEFANELO, N., MORSCH, V. M., AKINDAHUNSI, A. A., OBOH, G. & SCHETINGER, M. R. 2018. Dietary supplementation of tiger nut alters biochemical parameters relevant to erectile function in L-NAME treated rats. Food Research International, 109, 358-367.

OLOJEDE, S. O., LAWAL, S. K., DARE, A., MOODLEY, R., RENNIE, C. O., NAIDU, E. C. & AZU, O. O. 2021. Highly active antiretroviral therapy conjugated silver nanoparticle ameliorates testicular injury in type-2 diabetic rats. Heliyon, 7, e08580.

OWEMBABAZI, E., NKOMOZEPI, P. & MBAJIORGU, E. F. 2023. Impact of Concurrent Exposure of Diabetic Male Sprague Dawley Rats to Alcohol and Combination Antiretroviral Therapy (cART) on Reproductive Capacity. Applied Sciences, 13, 5096.

SAHINER, U. M., BIRBEN, E., ERZURUM, S., SACKESEN, C. & KALAYCI, Ö. 2018. Oxidative stress in asthma: Part of the puzzle. Pediatric Allergy and Immunology, 29, 789-800.

SAVASI, V., PARISI, F., ONETA, M., LAORETI, A., PARRILLA, B., DUCA, P. & CETIN, I. 2019. Effects of highly active antiretroviral therapy on semen parameters of a cohort of 770 HIV-1 infected men. PLoS One, 14, e0212194.

SCHULTE, B. A., HARLEY, R. A. & SPICER, S. S. 2020. Carbohydrate histochemistry. Models of Lung Disease. CRC Press.

SEDLAK, J. & LINDSAY, R. H. 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical biochemistry, 25, 192-205.

SHARIF, H., AKASH, M. S. H., REHMAN, K., IRSHAD, K. & IMRAN, I. 2020. Pathophysiology of atherosclerosis: Association of risk factors and treatment strategies using plant‐based bioactive compounds. Journal of Food Biochemistry, 44, e13449.

SIMONI, M. & SANTI, D. 2020. FSH treatment of male idiopathic infertility: Time for a paradigm change. Andrology, 8, 535-544.

SINGH, R., GUPTA, A. K. & KAKA, K. 2018. Traditional medicinal plants as scientifically proven Aphrodisiacs. International Journal of Health and Biological Sciences, 1, 29-36.

SOFOWORA, A. 1993. Medicinal plants and traditional medicine in Africa. Spectrum Books Limited. Ibadan, Nigeria, 1-153.

TREZZA, C. R. & KASHUBA, A. D. 2014. Pharmacokinetics of antiretrovirals in genital secretions and anatomic sites of HIV transmission: implications for HIV prevention. Clinical pharmacokinetics, 53, 611-624.

UDEFA, A. L., AMAMA, E. A., ARCHIBONG, E. A., NWANGWA, J. N., ADAMA, S., INYANG, V. U., INYAKA, G. U.-U., AJU, G. J., OKPA, S. & INAH, I. O. 2020. Antioxidant, anti-inflammatory and anti-apoptotic effects of hydro-ethanolic extract of Cyperus esculentus L.(tigernut) on lead acetate-induced testicular dysfunction in Wistar rats. Biomedicine & pharmacotherapy, 129, 110491.

VERA, Y., DIAZ-ROMERO, M., RODRIGUEZ, S., LUE, Y., WANG, C., SWERDLOFF, R. S. & SINHA HIKIM, A. P. 2004. Mitochondria-dependent pathway is involved in heat-induced male germ cell death: lessons from mutant mice. Biology of reproduction, 70, 1534-1540.

WEIBEL, E. R. 2020. Morphometry: stereological theory and practical methods. Models of lung disease. CRC Press.

Published

2023-12-31

Issue

Section

Full Length Research Articles

How to Cite

Evaluation of Testicular Function and Structural Changes of Wistar Rats Following Antiretroviral Exposure: Protective Role of Cyperus Esculentus. (2023). Nigerian Journal of Physiological Sciences, 38(2), 201-209. https://doi.org/10.54548/njps.v38i2.9

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