Comparative Effects of Methanol and Aqueous Extracts of Corchorus olitorius Plant on Haematology and Some Reproductive Indices of Male Albino Rats (Wistar Strain)


Corchorus olitorius is a vegetable plant/shrub and the leaves are very nutritious and rich in vitamins, minerals and dietary fibers. The study was carried out to identify the effects two extracts of this plant on the reproductive indices of male Wistar rats

Forty-five adult male rats of about 12 weeks old, each weighing about 120g were used for this study, kept in a cage and fed with commercial rat pellets and water was given at ad-libitum. They were randomly divided into groups A, B and C of 15 rats per group. 250mg/kg of the leaf extract was administered orogastrically using once daily for 21 days. Group A and B were treated with methanol and aqueous leaves of Corchorus extract respectively and Group C was given distilled water and served as the control. The weekly sacrifice of five rats per group was done following

treatments. In this study, it was observed at the first-week post -treatment that there was significant (p≤0.05) decrease in the PCV and haemoglobin values of group A rats compared to the control group but the values later increased at the second week and third-week post-treatments. There was also a significant decrease in spermatozoa motility in the treated groups A and B compared to the control group C at the first, second- and third-week post treatments. Also, with the results of the serum testosterone level of group A and B compared to group C. The value was higher in group A followed by group B whereas, at the second week and third week, there was no significant difference in the values of the serum testosterone levels compared to the control groups.

It is therefore concluded that the methanol and aqueous extract of Corchorus olitorius leaves significantly decreased sperm motility in male albino rats hence could decrease male fertility. 

Click to view file (PDF)


Al Batran R, Al-Bayaty F, Abdulla MA, Al-Obaidi MM, Hajrezaei M, Hassandarvish P, Fouad M, Golbabapour S, Talaee S. (2013). Gastroprotective effects of Corchorus olitorius leaf extract against ethanol-induced gastric mucosal hemorrhagic lesions in rats. J Gastroenterol Hepatol. ;28(8):1321-9.

Azuma K, Nakayama K, Koshioka M, Ippoushi K, Yamaguchi Y, Kohata K, Yamauchi Y, Ito H, Higashi H. Phenolic antioxidants from the leaves of Corchorus olitorius L. J Agric Food Chem 1999; 47:3963-3966. 14.

American Diabetes Association (2000): Nutrition recommendation and principles for people with diabetes mellitus clinical practice recommendations Diabetes care 23:543-6.

Barku V.Y.A., Boye A. and Quansah N. (2013). Antioxidant and wound healing studies on the extracts of Corchorus olitorius leaf. World Essays Journal 1 (3): 67-73.

Casteleyn, Christophe; Trachet, Bram; Van Loo, Denis; Devos, Daniel G H; Van den Broeck, Wim; Simoens, Paul; Cornillie, Pieter (2017). "Validation of the murine aortic arch as a model to study human vascular diseases". Journal of Anatomy. 216 (5): 563–571.

Chauhan A, Agarwal M. (2008). Reversible changes in the antifertility induced by Aegle marmelos in male albino rats. Syst Biol Reprod Med ; 54: 240–6.

Chopra. R. N., Nayar. S. L. and Chopra. I. C.(1986). Glossary of Indian Medicinal Plants (Including the Supplement). Council of Scientific and Industrial Research, New Delhi.1986ISBN

Clause, B. T. (1998). "The Wistar Institute Archives: Rats (Not Mice) and History", Mendel Newsletter February, 1998. Archived 2006-12-16 at the Wayback Machine.

Coles, E. H. (1986) Veterinary clinicalpathology. 4th edition (ed E. H. Coles) W. B. Saunders Compny. Philadelphia.

Duke, James A. (1979). "Ecosystematic Data on Economic Plants". Quarterly Journal of Crude Drug Research.17 (3-4): 91–109. doi:10.3109/13880207909065158. ISSN 0033-5525.

Duke James.A. (1983). Corchorus olitorius L. Handbook of Energy Crops. unpublished.

Das A.K., Sahu R., Dua T.K. and Baq S. (2010). Arsenic-induced myocardial injury: protective role of Corchorus olitorius leaves. Food Chem Toxicol 48 (5): 1210-7.

De Gruchy G.C. (1976): Clinical haematology in Medical Practice. Blackwell Scientific Publication. Oxford, London pp. 33-57.

Facciola. S. Cornucopia - A Source Book of Edible Plants. Kampong Publications 1990 ISBN 0-9628087-0-9

Foote, Allison L.; Jonathon D. Crystal (2007). "Metacognition in the rat". Current Biology. 17 (6): 551–555.

Furumoto T, Wang R, Okazaki K, Hasan AFMF, Ali MI, Kondo A, Fukui H. (2002). Antitumor promoters in leaves of Jute (Corchorus capsularis and Corchorus olitorius) Food Sci. Technol. Res. 8 (3) 239-243.

Grubben, G. J. H. (2004). Vegetables. PROTA. ISBN 9789057821479.

Holm, LeRoy G. New York: Wiley. (1997). World weeds: Natural histories and distribution. Holm, LeRoy G. New York: Wiley. 1997. ISBN 9780471047018. OCLC 34114783.

Ilhan Semra, filiz savaroğlu, ferdağ çolak (2007). Antibacterial and Antifungal Activity of Corchorus olitorius L. (Molokhia) Extracts. International Journal of Natural and Engineering Sciences 1 (3): 59-61.

Jain, N. C. (1986) Schalm’s veterinary haematology 4th Edition (ed N. C. Jain) ea and Febiger, Philadelphia pp 1221.

Manzoor I, Khuda M, Islain A. Chemical constituents of Corchorus olitorius and Corchorus cpsularis (jute) II. Isolation of corosin and sitosterol from roots. Pak J Sci Ind Res 1971; 14:49-56. 10.

Mukherjee KK, Mitra SK, Ganguli SN. (1998) A new coumarin from the seeds of jute (Corchorus olitorius L.). Nat Prod Sci 1998; 4:51-52. 15.

Nyadanu, D.; Amoah, R. Adu; Kwarteng, A. O.; Akromah, R.; Aboagye, L. M.; Adu-Dapaah, H.; Dansi, A.; Lotsu, F.; Tsama, A. (2017). "Domestication of jute mallow (Corchorus olitorius L.): ethnobotany, production constraints and phenomics of local cultivars in Ghana". Genetic Resources and Crop Evolution. 64 (6): 1313–1329..

Nishiumi S., Yabushita Y., Fukuda I., Mukai R., Yoshida K. and Ashida H. (2005). Molokhia (Corchorus olitorius L) extract suppresses transformation of the aryl hydrocarbon receptor induced by dioxins. Food Chem Toxicol; 44 (2): 250-60.

Oboh G, Raddatz H, Henle T. 2009. Characterisation of the antioxidant properties ofhydrophilic and lipophilic extracts of Jute leaf Corchorus. International journal of food sciences and nutrition, 60(S2):124-34.

Olatunji-Bello I.I., Ijiwole T, Awobajo F.O. (2009). Evaluation of the deleterious effects of aqueous fruit extract of Abelmoschus esculentus (Okro fruit) ond some male reproductive parameters in sprague dawley rats. J Phytol

Oyedeji, K.O. Bolarinwa, A.F. Akinbode A.A. (2013). Effect of Corchorus Olitorius Extract On Reproductive Functions In Male Albino Rats / International Journal of Pharmacy and Pharmaceutical Sciences, Vol 5, Issue 3,

Oyedele D.J. (2006). Heavy metals in oil and accumulation by edible vegetables after phosphate fertilizer application. Elect. J. Agricultural Food Chem., 5:14461453 References AOAC 1990. Official Methods of Analysis. 14th edition, Association of Official Analytical Chemists, Washington DC.

Oyeyemi. M.O. and Adetunji. P. Fayomi (2011). Reproductive potential of male Wistar rat treated for short term with graded concentration of Talimum Triangulare crude extract. Global Veterinaria 7 (2) 188-191.

Polenakovic M. and Sikole A. (1996): Is erythropoietin a survival factor for red blood cells? J. Am. Soc Nephrol 7(8): 1178-1182.

Raji Y, Bolarinwa AF. Antifertility activity of Quassia amara in male rats– in vivo study. Life Sci 1997; 61: 1067–74. es. 17(3-4):91-110.

Rao EV, Rao KN, Rao DV, Polar glycosides of the seeds of Corchorus olitorius. Ind J Pharm 1972; 34:168.

Sanilova RD, Lagodich TA. Olitoriside - A glycoside from the seeds of Corchorus olitorius. Urech Delo 1977; 1:2731. 6

Tanmoy AM, Alum MA, Islami MS, Farzana T, Khan H. Jute (Corchorus olitorius var. O-72) stem lignin: variation in content with age. Bangladesh J. Bot. 2014; 43(3):309-314, 16.

Tindall. H. D (1983). An excellent, in-depth look at the main vegetable crops that can be grown in the Tropics, plus many less well-known plants. MacMillan, Oxford.1983ISBN0-333-24268-8

Vandenbergh, J. G. (2000). "Use of House Mice in Biomedical Research". ILAR Journal. 41 (3): 133–135.

Pal, DK; Gupta, M; Mazumder, UK. (2009). Effects of methanol extracts of Cuscuta reflexa Roxb. stem and Corchorus olitorius Linn. seed on male reproductive system of mice. Oriental Pharmacy and Experimental Medicine. Volume 9, Issue 1, 2009, pp.49-57, Kyung Hee Oriental Medicine Research Center, Kyung Hee University.

Wallingford, J.; Liu, K.; Zheng, Y. (2010). "MISSING". Current Biology. 20: R263–4.,

Yoshikawa M, Shimada H, Saka M, Yoshizumi S, Yamahara J, Matsuda H. Medicinal Foodstuffs. V Moroheya (1): Absolute stereostructures of corchoionosides A, B and C, histamine inhibitors from the leaves of Vietnamese Corchorus olitorius L. (Tiliaceae). Chem Pharm Bull 1997; 45:464469. 13.

Zemjanis, R (1970). Collection and Evaluation of semen. In: Diagnostics and Therapeutic Techniques in Animal Reproduction, 2nd Edition, Williams and Wilson Co., Baltimore, MD 139-340.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2021 Nigerian Journal of Physiological Sciences