Influence of nanosilver on osmotic fragility responses of erythrocyte membrane following Na+/K+-ATPase blockade


Nanosilver-induced lysis in Na /K ATPase blockade


Background: The molecular mechanisms and overt effects of nanoparticle-induced changes in
red blood cells (RBCs) structure and function across membrane cell lines remain unclear
despite the increasing use and application in nanomedicine. The aim of this study was to
assess the impact of nanosilver exposure on osmoregulation of red cell membrane fragility in
digoxin-induced Na+-K+ATPase blockade in vitro. Materials and Method: Samples from 50
subjects were obtained from consenting asymptomatic adults: male and female HbAA
haemoglobin genotype. After separation and washing of erythrocytes, the samples were
divided into three sets with each sample treated in duplicate with graded percentage
concentrations of phosphate buffer solutions (0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1). The
second and third set of samples were incubated with 0.05ml of erythrocytes, 1 ml phosphate
buffer saline and 1 ml nanosilver or digoxin of 25 mg/ml. Thereafter, the content of each test
tube was incubated for 1 hour and 3 hours respectively. The absorbance was recorded after
30mins incubation for each set with standard spectrophotometer at 540 nm wavelength.
Haemolysis in each tube was recorded and expressed as percentage of the absorbance in
distilled water. The average values recorded were plotted against the different concentrations
used. Results: Erythrocytes from the sample incubated with nanosilver had significantly
increased osmotic lysis compared with the untreated cells in rate-depended manner (P<0.05).
Similar pattern was observed with digoxin pre-incubated cells. The mean osmotic fragility
(MOF) index of the untreated, nanosilver and digoxin pre-incubated cells was in the order:
digoxin>nanosilver>untreated. Conclusion: exposure of RBCs to nanosilver and in
Na+/K+ATPase blockade may result in increased hemolytic effects by multifactorial cell
membrane-mediated processes