Öz
Background: Garcinia cambogia is a plant with reported spermatogenic properties. Ciprofloxacin is an antibiotic used in the treatment of various bacterial infections but with reported toxic effects on the testes. The modulatory effects of Garcinia cambogia on ciprofloxacin-induced testicular toxicity was evaluated in this study.
Method: Twenty (20) male Wistar rats were randomly divided into four (4) groups. Group A was given Ciprofloxacin 150 mg/kg only, Group B was given 150 mg/kg Ciprofloxacin and 400 mg/kg Garcinia cambogia, Group C was administered 400 mg/kg Garcinia cambogia only while Group D served as the normal control group and received distilled water (placebo). Administration lasted for 30 days and thereafter the animals were sacrificed by cervical dislocation; blood samples were collected for hormonal studies; and the testes harvested and processed for histological studies.
Results: Findings obtained showed significantly reduced concentration of luteinizing and follicle stimulating hormones in ciprofloxacin-treated group compared to the Garcinia cambogia-treated and control groups at p<0.05. The concentration of testosterone in ciprofloxacin + Garcinia cambogia-treated group was significantly higher than in the ciprofloxacin-treated group at p < 0.05. Histological analysis revealed improved seminiferous tubule architecture in Garcinia cambogia treated rats compared to the ciprofloxacin-treated group.
Conclusion: The results show that Garcinia cambogia extract has spermatogenic properties mediated through its influence on pituitary hormones, thereby ameliorating ciprofloxacin-induced testicular toxicity.
Keywords: Garcinia cambogia, ciprofloxacin, testes, follicle stimulating hormone, luiteinising hormone, testosterone
Résumé
Contexte: Garciniacambogia est une plante avec des propriétés spermatogénesrapportés. La ciprofloxacine est un antibiotique utilisé dans le traitement de diverses infections bactériennes, mais avec des effets toxiques rapportés sur les testicules. Les effets modulateurs de Garciniacambogia sur la toxicité testiculaire induite par la ciprofloxacine a été évaluée dans cette étude.
Méthode: Vingt (20) rats mâles Wistar ont été divisés au hasard en quatre (4) groupes. Groupe A a été donné Ciprofloxacin 150 mg / kg seulement, groupe B a reçu 150 mg / kg de ciprofloxacine et 400 mg / kg de Garciniacambogia, groupe C a été administré 400 mg / kg de Garciniacambogia seulement tandis que le groupe D a servi comme groupe de contrôle normal et reçu de l’eau distillée (placebo). L’administration a duré 30 jours etdans la suite les animaux ont été sacrifiés par dislocation cervicale; des échantillons de sang ont été prélevés pour des études hormonales; et les testicules récoltées et traitées pour des études histologiques.
Résultats: Les résultats obtenus ont montré la concentration significativement réduite de lutéinisante et hormone folliculo-stimulante dans legroupetraité avecciprofloxacine par rapport aux groupestraités avec Garciniacambogia et de contrôle à p <0,05. La concentration de testostérone dans la ciprofloxacine + Garciniacambogia groupe traité était significativement plus élevée que dans le groupe ciprofloxacine traités à p <0,05. L’analyse histologique a révélé une amélioration de l’architecture des tubules séminifères chez les rats traités avec Garciniacambogia par rapport au groupe traité par la ciprofloxacine.
Conclusion: Les résultats montrent que l’extrait de Garcinia cambogia a des propriétés spermatogènes médiées par son influence sur les hormones hypophysaires, ainsiaméliorant la toxicité testiculaire induite par la ciprofloxacine.
Mots-clés: Garcinia cambogia, ciprofloxacine, testicules, hormones folliculo-stimulantes, hormones lutéinisantes, testosterone
Correspondence: Dr. Ukwenya Victor, Department of Anatomy, School of Health and Health Technology, Federal University of Technology, Akure, Nigeria. E-mail: voukweya@futa.edu.ng
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