The presence of some TNFα and receptor polymorphic forms might reduce the risk of breast cancer in Nigeria
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Resumé
Introduction: Breast Cancer remains a global health
problem and therefore its early detection and good
diagnostic biomarkers are important for successful
treatment and prevention. Tumour necrosis factor
alpha Single Nucleotide Polymorphisms
(TNFαSNPs) have been implicated in the
presentation of some human cancers. This
preliminary study was carried out to determine for
the first time, the role of TNFα SNPs in breast cancer
amongst Nigerian women and its possible use as risk
assessment tool for breast cancer.
Methods: Participants (200) were recruited from the
University College Hospital, Ibadan for this study.
Genotyping of TNFα and its receptor SNPs from
purified DNA in breast cancer and control samples
were carried out using Polymerase Chain Reaction -
Touch down technique with allele specific primers.
Results: Five alleles showed a significant association
for a reduced risk of breast cancer TNFα 488G
(P=0.0140 OR=0.236, 95%CI= 0.07542 to 0.7391),
TNFα 380G (P=0.0369, OR= 0.5108, 95%CI=
0.5108 to 0.9251), TNFα 308A P=0.0149, OR =
0.3271, 95%CI=0.1373 to 0.7794), and
TNFR1A+IV56+10 -G (P= 0.0024, OR= 0.3535,
95%CI= 0.1851 to 0.6753) TNFα 1032C(P= 0.0158,
OR=2.077 CI=(1.180 to 3.653) with very high
heterozygosity observed among SNPs
Conclusion: Specific genotypic variations in TNFα-
SNPs might be a potential risk factor for breast cancer
among Nigerian population, raising the possibility of
the use of TNFα-SNPs as bio-markers for early risk
indicators for breast cancer in Nigeria.
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Referencer
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