Exogenous administration of adenosine enhanced glucose uptake in canine hind limb at rest and during contraction
Abstract
Background: Glucose metabolism increases during contraction of skeletal muscle and can be influenced by the endogenous adenosine. However, the role of exogenous adenosine in regulating glucose uptake at rest or during contraction has not been elucidated in dogs. We studied the effects of exogenous adenosine on glucose uptake in canine hind limb at rest and during contraction.
Methods: The study was carried out using thirty (30) fasted and anaesthetized male dogs divided into six groups (5dogs/group). Groups I (control) and II received normal saline (0.1ml/kg) at rest and during contraction of hind limb respectively. Group III received adenosine (0.1, 0.5 and 1mg/kg) at rest. Group IV were treated with adenosine (1mg/kg) during contraction. Groups V and IV were pretreated with caffeine (6mg/kg) and infused with adenosine (1mg/kg) for thirty minutes at rest and during contraction of the hind limb respectively. Blood glucose was measured by glucose oxidase method. Arterio-venous (A-V) glucose and venous blood flow (VBF) were measured; hind limb glucose uptake (HGU) was calculated as the product of A-V glucose and VBF.
Results: The results showed that exogenously administered adenosine significantly (P<0.05) increased AV glucose, VBF and HGU in a dose dependent manner at rest. During contraction adenosine increased AV glucose significantly from14.2±0.5mg/dl to 45.4±1.8ml/min. VBF also significantly increased from 4.7±0.6ml/min to 16.3±1.2 and HGU from 34.8±2.4 to 450.8±8.2mg//min. Pretreatment with caffeine significantly reduced adenosine-induced hyperglycemia at rest and during contraction.
Conclusion: Exogenous adenosine at rest and during contraction increases the skeletal muscle glucose uptake and the increase appears to be mediated by inhibition of adenosine receptors.
Keywords: Adenosine, Caffeine. Dog, glucose uptake, hind limb
Résumé - 3710
Contexte: Le métabolisme du glucose augmente pendant la contraction du muscle squelettique et peut être influencé par l’adénosine endogène. Cependant, le rôle de l’adénosine exogène dans la régulation de l’absorption de glucose au repos ou pendant la contraction n’a pas été élucidée chez les chiens. Nous avons étudié les effets de l’adénosine exogène sur l’absorption de glucose dans le membre postérieur canin au repos et pendant la contraction.
Méthodes: L’étude a été menée à l’aide de trente (30) chiens mâles en jeûnés et anesthésiés répartis en six groupes (5 chiens / groupe). Les groupes I (témoin) et II ont reçu une solution saline normale (0,1 ml / kg) au repos et pendant la contraction du membre postérieur respectivement. Le groupe III a reçu de l’adénosine (0,1 ; 0,5 et 1 mg / kg) au repos. Le groupe IV a été traité avec de l’adénosine (1 mg / kg) pendant la contraction. Les groupes V et IV ont été prétraités avec de la caféine (6 mg / kg) et infusés avec de l’adénosine (1 mg / kg) pendant 30 minutes au repos et pendant la contraction du membre postérieur respectivement. La glycémie a été mesurée par la méthode de l’oxydase du glucose. Le glucose sanguin artério-veineux (A-V) et le flux sanguin veineux (VBF) ont été mesurés; l’absorption de glucose des membres postérieurs (HGU) a été calculée comme le produit du glucose A-V et du VBF.
Résultats: Les résultats ont montré que l’adénosine administrée exogène de manière significative (P <0,05) a augmenté le glucose A-V, VBF et HGU de manière dépendante de la dose au repos. Pendant la contraction, l’adénosine a augmenté le glucose A-V significativement de 14,2 ± 0,5 mg / dl à 45,4 ± 1,8 ml / min. VBF a également augmentée de 4,7 ± 0,6 ml / min à 16,3 ± 1,2 et HGU de 34,8 ± 2,4 à 450,8 ± 8,2 mg / min. Le prétraitement avec la caféine a considérablement réduit l’hyperglycémie induite par l’adénosine au repos et pendant la contraction.
Conclusion: L’adénosine exogène au repos et pendant la contraction augmente l’absorption du glucose dans le muscle squelettique et l’augmentation semble être par la médiation de l’inhibition des récepteurs de l’adénosine.
Mots-clés: Adénosine, Caféine. Chien, absorption de glucose, membre postérieur
Correspondence: Dr. H.M. Salahdeen, Department of Physiology, Lagos State University College of Medicine, Ikeja, Lagos, Nigeria. Email: hmsalahdeen@gmail.com; hussein.salahdeen@lasucom.edu.ng
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