Manniophyton fulvum is a herb with claimed anti-dysmenorrhea properties ascribed to the leaves in ethnomedicine. This study aimed to determine the effects of the methanol leaf extract and fractions on isolated mouse uterine contraction. Methanol extract of the leaf was obtained by Soxhlet extraction and partitioned into chloroform and water. The isolated and mounted mouse uterine tissues were treated with the extract, chloroform and aqueous fractions at a concentration range of 0.0007 – 7.777 µg/ml. The chloroform and aqueous fractions were equally tested on oxytocin and potassium chloride pre-contracted uterine tissue at a concentration range of 0.34 – 34.3 µg/mL. LC-HRFTMS analysis of methanol extract and the aqueous fraction was also carried out. The extract and aqueous fraction were observed to significantly (p< 0.05) increase the frequency and amplitude of spontaneous contractions in a concentration-dependent manner, while the chloroform fraction concentration-dependently inhibited similar contractions. The aqueous fraction potentiated oxytocin and potassium chloride augmented contractions in a similar manner, while the chloroform fraction significantly inhibited augmented contractions. The spectroscopic analysis identified eight and nine compounds in the methanol extract and aqueous fraction respectively, belonging to the coumarin, terpenoid, and flavonoid classes of natural compounds. Evidence from this study suggests that constituents responsible for uterine contraction are present in the polar (aqueous) fraction of the extract, while the non-polar fraction (chloroform) contains compounds with uterine relaxing effects.
Akinbo F. O., Okaka C. E. (2010): Hyperendemicity of Onchocerciasis in Ovia North East Local Government area, Edo State, Nigeria. Malay. J Med Sci. 17, 20-24.
Arrowsmith S., Wray S. (2014): Oxytocin: its mechanism of action and receptor signalling in the myometrium. J Neuroendocrinol. 26, 356-69.
Bafor E. E., Nwogu J. K., Elvis-Offiah U. B., Amaechina F., Ofeimun J., Ayinde B., Omoruyi O., Viegelmann C., Edrada-Ebel R. (2018a): Modulation of ex-vivo uterine contraction by the methanol leaf extract of Alchornea laxiflora Benth. (Euphorbiaceae) and preliminary spectrometric identification of associated secondary metabolites’, J Med Plants Econ Develop. 2018;2(1), a33. https://doi. org/10.4102/jomped.v2i1.33.
Bafor E. E., Rowan E. G., Edrada-Ebel R.. (2018b): Metabolomics-Coupled Functional Pharmacology of Chlorophyll Compounds Isolated From the Leaves of Ficus Exasperata Vahl (Moraceae) Provides Novel Pathways on Myometrial Activity. Reprod. Sci. 25, 923–937.
Bafor E. E., Obarisiagbon P.A., Itamaomon J. L (2016): Investigation of the myometrial stimulatory effect of amide anaesthetics. J Pharmaceut Allied Sci. 12, 2191–2209.
Banikarim C., Chacko M. R., Kelder S. H. (2000) Prevalence and impact of dysmenorrhea in Hispanic female adolescent. Arch Pediiatr Adolesc Med. 154, 1226 – 1229.
Beck S., Wojdyla D., Say L., Betran A. P., Merialdi M., Requejo J. H., et al (2000). The worldwide incidence of preterm birth: a systematic review of maternal mortality and morbidity. Bull World Health Org. 88, 31-38.
Burkill, H. M. (1994). The useful plants of West Tropical Africa. 2nd edidition. Volume 2, Families E-I. pp 36 ; Royal Botanic Gardens, Kew, Richmond, United Kingdom.
Caligioni C. S. (2009): ‘Assessing reproductive status/stages in mice’, Curr. Protocol Neurosci. Appendix 4, Appendix 4I. https://doi.org/10.1002/0471142301. nsa04is48 Accessed 23 June 2018.
Coco A. S. (1996): Primary dysmenorrhea. Am. Fam Physician. 60, 489-496.
de Alencar Cunha K. M., Paiva L.A.F., Santos F.A., Gramosa N.V., Silveira E. R., Rao V.S. N., (2013): Smooth muscle relaxant effect of kaurenoic acid, a diterpene from Copaifera langsdorfﬁi on rat uterus in vitro. Phytotherapy Res. 17, 320-324.
Egua M. O, Etuk E. U., Bello S. O., Hassan S. W. (2014): Antidiabetic potential of liquid-liquid partition fractions of ethanolic seed extract of Corchorus olitorious J Pharmacog Phytother. 6, 4-9.
Emudainohwo J.O T., Ejebe D.E., Erhirhie E. O., Moke E. G., (2010): Investigation into the aphrodisiac properties of aqueous and ethanol root extracts of Manniophyton fulvum in male Wistar rats. IOSR J Pharm Biolog. Sci 10, 01-06.
Fedorowicz Z., Nasser M., Jagannath V. A., Beaman J. H, Ejaz K., van Zuuren E. J. (2012): Beta2-adrenoceptor agonists for dysmenorrhoea. Cochrane Database Syst Rev. 16, CD008585.pub2.
Garfield R. E., Maner W. (2007): Physiology and electrical activity of uterine contractions Semin. Cell. Dev. Biol. 18, 289-295.
Gruber C. W, O.Brien M. (2011): Uterotonic plants and their bioactive constituents . Planta Med. 77, 207-220.
Jia S., Shen M., Zhang F., Xie J. (2017): Recent Advances in Momordica charantia: Functional Components and Biological Activities. Int. J Mol. Sci. 18, 25-35.
Kabilan A. (2014): Pharmacological role of Oxytocin-A short review. J Pharmaceut Sci. Res. 6, 220 – 223.
Kupittayanant S., Munglue P., Lijuan W., Promprom W., Budhaklala N., Wray S. (2014): Finding new agents in medicinal plants to act on the myometrium. Exp Physiol. 99, 530-537.
Luu T. M., Katz S. L., Leeson P., Thébaud B., Nuyt A. (2016): Preterm birth: risk factor for early-onset chronic diseases. Can Med Ass J. 188, 736-746.
Majoribanks J., Ayeleke R. O., Farquhar C., Proctor M. (2015): Nonsteriodal anti-inflammatory drugs for dysmenorrhea. Conchrane database of Syst Rev. 2015; (1):CD001751 DOI:10.1002/14651858.CD001751.pub3.
Mirabi P., Alamolhoda S. H., Esmaeilzadeh S., Mojab F. (2014): Effect of Medicinal Herbs on Primary Dysmenorrhoea- a Systematic Review. Iranian J of Pharmaceut Res. 13, 757-767.
Mozurkewich E., Chilimigras J., Koepke E., Keeton K., King V. J (2009): Indications for induction of labour: a best-evidence review. Brit J Obst. Gyneacol. 116, 626-36.
Nai R., Paper D., Franz G., Hohmann G., Essien E. E, Muganza M. (2005): Anti-oxidant and Anti-inflammatory Activity of Manniophyton fulvum. P,(4) Acta Hortic 678, 97 – 105.
National Institute of Health (2015): Public health service policy on humane care and use of laboratory animals, Office of Laboratory Animal Welfare. http:// grants.nih.gov/grants/olaw/references/PHSPolicyLabAnimals.pdf. Accessed, November 21st 2018.
Ojieh E. A., Adegor C. E., Ovuakporaye I. S., Ewhre O. L. (2013): Preliminary Phytochemical screening and antidiarrheal properties of Manniophyton fulvum. J Dent. Med Sci. 10, 46-52.
Oladosu F. A., Tu F. F., Hellman K. M (2017): Non-steroidal anti-inflammatory drug resistance in dysmenorrhea; epidemiology, causes and treatment. Am. J of Obstet. Gynecol. 218, 390 – 400.
Omokhafe A. U., Soladoye M. O., Baiyewu R. A., Ibhanesebhor G. A. (2012) Vernacular names of some Nigerian Medicinal plants: Edo/Delta version: pp11,75. Forest Research Institute of Nigeria, Ibadan.
Onyemairo N. J., Patrick-Iwuanyanwu K. C., Mango C. C (20120 Evaluation of antidiabetic properties of Manniophyton fulvum. J Biol Gen Develop. 8, 23 – 30.
Patel S. S., Ludmir J. (2019): Drugs for the Treatment and Prevention of Preterm Labor. Clin Perinatol. 46, 159-172.
Patel V, Tanksale V, Sahasrabhojanee M, Gupte S, Nevrekar P. (2006): The burden and determinants of dysmenorrhoea: a population-based survey of 2262 women in Goa, India. Brit J Obst Gyneacol. 2006; A113(4):453-63.
Phillippe M., Chien E. K. (1995): Potassium chloride effects on the hormonal signal transduction mechanisms underlying phasic myometrial contractions. J Endocrinol. 146, 485-93.
Ponce-Monter H., Perez S., Zavala M. A. Perez C., Meckes M., Macias A., Campos M. (2006): Relaxant effect of xanthomicrol and 3alpha-angeloyloxy-2alpha-hydroxy-13,14z-dehydrocativic acid from Brickellia paniculata on rat uterus. Biol Pharm Bull. 29, 1501-1503.
Proctor M. L., Farquhar C. (2006): Diagnosis and management of dysmenorrhea Brit. Med. J 332, 1134 – 1138.
Proctor M. L., Farquhar C. (2006). Diagnosis and management of dysmenorrhea. Brit Med J. 332: 1134 – 1138.
Proctor M. L., Roberts H., Farquhar C. M. (2001): Combined oral contraceptive pill (OCP) as treatment for primary dysmenorrhoea. Cochrane Database Syst Rev. 4, CD002120. doi: 10.1002/14651858.CD002120.
Pulkkinen M. O. (1983): Prostaglandins and the non- pregnant Uterus. The pathophysiology of primary dysmenorrhea. Acta Obs Gynecol Scand. Suppl. 113, 63 – 67.
Sadraei H., Shokoohinia Y., Sajjadi S. E., Ghadirian B. (2012): Antispasmodic effect of osthole and Prangos ferulacea extract on rat uterus smooth muscle motility. Res. Pharmaceut. Sci. 7, 141–149.
Sharma A., Taneja D. K, Sharma P., Saha R. (2008): Problems related to menstruation and their effect on daily routine of students of a medical college in Delhi, India. Asia Pac J Pub. Health. 20, 234-241.
Sultan C., Gaspari L., Paris F. (2012): Adolescent dysmenorrhea. Endo. Develop. 22, 171 - 180.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2023 African Journal of Biomedical Research