Effect of African Walnut (Tetracarpidium conophorum) Seed Oil on 3-Methylcholanthrene-Induced Mammary Carcinogenesis and Expression of COX-2 and PPAR-ɣ in Female Wistar Rats
DOI:
https://doi.org/10.4314/ajbr.v25i2.15Keywords:
Tetracarpidium conophorum, 3-methylcholanthrene, cyclooxygenase-2. mammary cancer.Abstract
Breast cancer is a commonly diagnosed disease among women, and dietary lipids has been implicated in its incidence. This study investigated the effect of feeding African walnut seed oil (AWSO) on 3-methylcholanthrene (MCA) induced mammary cancer and expression of cyclooxygenase-2 (COX-2) and peroxisome proliferator activated receptor gamma (PPAR-ɣ) in female Wistar rats. AWSO was extracted with n-hexane in a Soxhlet apparatus and characterized by gas chromatography. Group A and B of 21 days old rats (15 each) were fed with diet containing 10% AWSO for 12 weeks. After 4 weeks of feeding, group A animals were administered MCA (250mg/kg) intraperitoneally. Another group (group C with 15 animals) was fed with diet containing no AWSO and administered MCA (250mg/kg) intraperitoneally after 4 weeks of feeding. Results revealed that animals fed with AWSO had lower tumor incidence (21.7%), tumor weight (1.22g) and tumor volume of 948mm3 compare to the animals not fed with AWSO (87.4%, 9.41g, 6281mm3, respectively. The expression of cyclooxygenase-2 was observed only in MCA treated animals and it was significantly less on AWSO fed group than on animals in group not fed with AWSO. The expression of PPAR-γ was significantly more on animals in group fed with AWSO than in group C (not fed with AWSO). Histological analysis of carcinogenesis were significantly (P < 0.05) more rapid on animals not fed with AWSO. Our results revealed that dietary AWSO reduce breast carcinogenesis induced by MCA.
References
Alugoju, P., Nyshadham, S. N., Krisnawamy, V.K. and Pavan, K. (2021). Theranostic precision medicine approach for female specific cancer: Phytotherapy for breast cancer. Pp129-163.
Ayeni, E. A. and Nuhu, A. (2018). Tetracarpidium conophorum (African walnut) Hutch and Dalziel: Ethnomedicinal uses and its therapeutic activities. Journal of Medicinal Plants for Economic Development, 2: 1-10.
Ayoola, P. B., Adeyeye, A., Onawumi, O. O. and Faboya, O. O. P. (2011). Phytochemical and nutrient evaluation of Tetracarpidium conophorum (Nigerian Walnut) Root. International Journal of Research and Reviews in Applied Sciences, 7: 197-208.
Badawi, A. F., EL-Sohemy, A., Stephen, L. L., Ghoshal, A. K. and Archer. M. C. (1998). The effect of dietary n-3 and n-6 polyunsaturated fatty acids on the expression of cyclooxygenase1 and 2 and level of p21ras in rat mammary glands. Carcinogenesis, 19; 905–910.
Cheng, W. L., Lii, C. K., Chen, H. W., Lin, T. H. and Liu, K. L. (2004). Contribution of conjugated linoleic acid to the suppression of inflammatory responses through the regulation of the NF-kappaB pathway. J. Agric. Food Chem., 52; 71–78.
Chijoke, O. C., Anosike, C. A. and Ani, C. C. (2015). Studies on the phytochemical and nutritional properties of Tetracarpidium conophorum (black walnut) seeds. Journal of Global Bioscience, 4: 1366-1372.
Chijoke, O.C., Assumpta, A.C., Lawrence, E. & Sunday, U., 2017, ‘Effect of black walnut (Tetracarpidium conophorum) leaf extract on the reproductive organ of male albino rats’, International Journal of Homeopathy and Natural Medicine 3(2), 9–14. https://doi.org/10.11648/j.ijhnm.20170301.12
Chinery, R., Beauchamp, R. D., Shyr, Y., Kirkland, S,, Coffey, R. J. and Morrow, J. D. (1998). Antioxidants reduce cyclooxygenase 2 expression? Prostaglandin production amd proliferation in colorectal cancer cells. Cancer Research, 58(11); 2323-2327.
Degner, S. C., Kemp, M. Q, Bowden, G.T., and Romagnolo, D. F. (2006). Conjugated linoleic acid attenuates cyclooxygenase-2 transcriptional activity via an anti-AP-1 mechanism in MCF-7 breast cancer cells. J. Nutr., 136; 421–427.
Harris, R. E., Robertson, F. M., Abou-Issa, H. M., Farrar, W. B. and Brueggemeier, R. (1999). Genetic induction and upregulation of cyclooxygenase (COX) and aromatase (CYP19): an extension of the dietary fat hypothesis of breast cancer. Med. Hypotheses., 52; 291–292.
Ihemeje, O., Ukauwa, C. C. and Ekwe, A. (2015). Effects of Cooking and Germination on Physiochemical Properties and Sensory Attributes of African Walnut (TETRACARPIDIUM CONOPHORUM) International Journal of Pharmacology, Phytochemistry and Ethnomedicine, 1; 93-100.
Kanu, A. M, Kalu, J. E., Okorie, A. C, Olabinri, B. M, Eniyansoro, O. O, et al., (2015). Evaluation of chelating ability of aqueous extracts of Tetracarpidium conophorum (African walnut) in vitro. International Journal of Applied Research in Natural Products, 3(3); 1-13.
Manni, G. and Caron, F. (1995). Calibration and Determination of Volatile Fatty Acids in Waste Leachates by Gas Chromatography. Journal of chromatography. 690: 237.
McCarty, M. F. (2000).Activation of PPAR gamma may mediate a portion of the anticancer activity of conjugated linoleic acid. Med Hypotheses. 55; 187–188.
Nwachoko, N. & Jack, I.R., (2015). ‘Phytochemical screening and anti-diarrhea activities of Tetracarpidium conophorum induced in albino rats’, Sky Journal of Biochemistry Research 4(4), 21–24.
Nwaichi, E.O., Osuoha, J.O. & Monanu, M.O., (2017). ‘Nutraceutical potential of Tetracarpidium conophorum and Buccholzia coriacea in diet-induced hyperlipidemia’, Journal of Chemical Health Risks 7(3), 157–170.
Nwauzoma, A. B. and Dappa M. S. (2013). ‘Ethnobotanicals studies of Port Harcourt metropolis, Nigeria’, ISRN Botany, pp 1–5.
Nwauzoma, A.B. & Dappa, M.S., (2013). ‘Ethnobotanicals studies of Port Harcourt metropolis, Nigeria’, ISRN Botany 1–5. https://doi.org/10.1155/2013/829424
Ogunyinka, B. I., Oyinloye, B. E., Adenowo, A. F. and Kappo, A.P. (2015). Potentials of some plant-derived foods in the management of diabetes and associated complications. African Journal of Traditional Complementary and Alternative Medicines, 12: 12–20.
Ojobor, C. C, Anosike, C. A, Ani C. C. (2015). Studies on Phytochemical and Nutritional Properties of Tetracarpidium conophorum (Black walnut) Seeds. Journal of Global Biosciences, 4(12):1366-1372.
Oluwole, S. O .and Okusanya, O. T. (1993). "Dormancy and seed germinationin the African walnut (TETRACARPIDIUM CONOPHORUM Mull.Arg)". J.Sci.Res.Dev., 1; 9-14.
Ram, S. and Geetanjali, T. (2018). Chemotaxonomy of Medicinal Plants. Natural Product and Drug Discovery. 6:119-136.
Rita, R., and Vinod, K. K. (2011). Study on cow ghee versus soybean oil on 7, 12-dimethylbenz (a)-anthracene induced mammary carcinogenesis & expression of cyclooxygenase-2 & peroxisome proliferators activated receptor- γ in rats. The Indian Journal of Medical Research, 135(5); 497-503.
Shiff, J.and Rigas, B. (1999). The role of cyclooxygenase inhibition in the antineoplastix effect of non-steroidal anti-inflammatory drugs (NSAIDS). Journal of Experimental Med., 190; 445-450.
Shou, M., Korzekwa, K. R., Krausz, K. W., Buters, J. T., Grogan, J., Goldfarb, I., et al., (1996). Specificity of cDNA-expressed human and rodent cytochrome P450s in the oxidative metabolism of the potent carcinogen 7, 12-dimethylbenz (a) anthracene. Mol. Carcinogen.,. 17; 241–249.
Shu-fang, N., Bao-Xia, C., Jun-Zhu, H. and Ying, G. (2017). PPARgamma is correlated with prognosis of epithelial ovarian cancer patients and affect tumor cell progressio in vitro. Int. J. Clin. Exp. Pathol., 10(3); 3235-3242
Uhunmwangho, E.S. and Omoregie, E.S. (2017b). Evaluation of nutritive, anti-nutritive and mineral content of Tetracarpidium conophorum (African Walnut) seed oil at different stages of fruit maturation. Haya: Saudi Journal Life Science, 2: 210-216.
Welsch, C. W. (1992). Relationship between dietary fat and experimental mammary tumorigenesis: a review and critique. Cancer Res., 52(7); 2040–2048
Wyk, B. V. and Wink, M. (2017). Medicinal Plants of the World. pp 7-8
Wynder, E. L., Rose, D. P. and Cohen, L. A. (1986). Diet and breast cancer in causation and therapy. Cancer. 58; 1804–1813.
Yasui, Y., Hosokawa, M., Sahara, T., Suzuki, R., Ohgiya, S., Kohno, H., et al. (2005). Bitter gourd seed fatty acid rich in 9c,11t,13t-conjugated linolenic acid induces apoptosis and up-regulates the GADD45, p53 and PPARgamma in human colon cancer Caco-2 cells. Prostaglandins Leukot. Essent. Fatty Acids. 73; 113–119.
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