Pathology and molecular detection of fowlpox virus in chickens in Nigeria
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Keywords

Fowlpox virus
polymerase chain reaction
histopathology
diagnosis

Abstract

Fowlpox is a relatively slow developing disease characterized by discrete, proliferative lesions on the skin of toes, legs or head, and/ or mucous membranes of the mouth and upper respiratory tract of birds. Several diseases of birds such as infectious coryza, infectious laryngotracheitis, Mareks, and vitamin A deficiency can be confused with fowlpox, therefore there is need for an accurate and reliable method of diagnosis. A combination of histopathology, Polymerase Chain Reaction (PCR) and sequence analysis can be used to arrive at the confirmatory diagnosis of the disease. Consequently, fifty scab samples from suspected cases of fowlpox from backyard poultry in Ibadan, Nigeria were collected, and PCR, sequencing with sequence analysis were employed to detect and characterize the open reading frame 168 (39 kDa core protein) gene of the virus. Also, lesions from the eyelids were excised and histopathology was carried out. Thirty-one out of 50 samples (62%) tested positive for fowlpox based on PCR and sequence analysis. Histopathology revealed the presence of dermal hyperplasia and Bollinger bodies which are confirmatory of fowlpox. PCR amplification, sequence analysis of 39 kDa core protein gene and histopathology provide a reliable diagnosis of fowlpox.

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References

Altschul SF, Gish W, Miller W. et al (1990): Basic Local Alignment Search Tool. J Mol Biol. 1990; 215, 403-410.

Bolte AL, Meurer J, and Kaleta EF (1999): Avian host spectrum of avipox viruses. Avian Pathol. 1999;28:415–432.

Boulanger D, Green P, Smith T, et al.(1998): The 131-Amino-Acid Repeat Region of the Essential 39-Kilodalton Core Protein of Fowlpox Virus FP9, Equivalent to Vaccinia Virus A4L Protein, Is Nonessential and Highly Immunogenic J. of Virol, 1998;Vol. 72, No. 1 p. 170–179.

Boulanger D, Green P, Jones B, et al (2002): Identification and characterization of three immunodominant structural proteins of fowlpox virus. J Virol. 2002; 76, 9844–9855.

Catroxo MH, Pongiluppi T, Melo N, et al. (2009): Identification of Poxvirus Under Transmission Electron Microscopy During Outbreak Period in Wild Birds, in São Paulo, Brazil. Int J Morpho. 2009; 27. 10.4067/S0717-95022009000200043.

Docherty DE, Long RI, Flickinger EL, et al. (1991): Isolation of poxvirus from debilitating cutaneous lesions on four immature grackles (Quiscalus sp.). Avian Dis. 1991;35:244–247.

Forrester DJ, Spalding MG 2003): Parasites and diseases of wild birds in Florida. Gainesville: University Press of Florida; 2003.

Gyuranecz M, Foster JT, Dán Á, et al. (2013): Worldwide phylogenetic relationship of Avian Poxviruses. J Virol. 2013; 87(9):4938–4951.

Hall TA. (1999): BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series. 1999; 41, 95-98.

Hess C1, Maegdefrau-Pollan B, Bilic I, Liebhart D, Richter S, Mitsch P, Hess M. (2011): Outbreak of cutaneous form of poxvirus on a commercial turkey farm caused by the species fowlpox. Avian Dis. 2011 Dec;55(4):714-8.

Jarmin S, Manvell R, Gough RE, et al. (2006): Avipoxvirus phylogenetics: identification of a PCR length polymorphism that discriminates between the two major clades. Journal of General Virology. 2006; 87, 2191–2201.

Lecis R, Secci F, Antuofermo E, et al. (2017): Multiple gene typing and phylogeny of avipoxvirus associated with cutaneous lesions in a stone curlew. Vet Res Commun. 2017; 41(2):77-83.

Manarolla G, Pisoni G, Sironi G, Rampin T. (2010): Molecular biological characterization of avian poxvirus strains isolated from different avian species. Vet Microbiol 2010;140:1–8.

Nakamura K, Waseda K., Yamamoto Y, et al. (2006): Pathology of cutaneous fowlpox with amyloidosis in layer hens inoculated with fowlpox vaccine. Avian Diseases. 2006; 50, 152–156.

Offerman K, Carulei O, Gous TA, et al. (2013): Phylogenetic and histological variation in avipoxviruses isolated in South Africa. J Gen Virol 2013; 94(Pt 10):2338–2351.

Ogasawara F, Yamamoto Y, Sato Y, Fukunari K, Murata K, Yaegashi G, Goto M, Murakami R (2016): Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius. Avian Dis. 60(3):705-8.

SaitoK, Haridy M, AbdoW, et al. (2019): Poxvirus infection in a Steller’s sea eagle (Haliaeetus pelagicus). J Vet Med Sci. 2019; 81(2): 338–342.

Sarker, S, Athukorala, A., Bowden, T.R. et al. (2021): Characterisation of an Australian fowlpox virus carrying a near-full-length provirus of reticuloendotheliosis virus. Arch Virol 166, 1485–1488.

Singh P, Schnitzlein WM, Tripathy DN. (2003): Reticuloendotheliosis virus sequences within the genomes of field strains of fowlpox virus display variability. J Virol. 2003; 77(10):5855-62.

Skinner MA, Laidlaw SM, Eldaghayes et al. (2005): Fowlpox virus as a recombinant vaccine vector for use in mammals and poultry. Expert Rev Vaccines. 2005; 4, 63-76.

Skinner MA and Laidlaw SM. (2009): Advances in fowlpox vaccination. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. 2009; 4, 1-10.

Tamura K, Peterson D, Peterson N, et al. (2011): molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–2739.

Thompson JD, Higgins DG, Gibson TJ. (1994): CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22: 4673–4680.

Tripathy DN, & Reed WM. Pox. In D.E. Swayne, J.R. Glisson, L.R. McDougald, L.K. Nolan, D.L. Suarez, & V. Nair (Eds.) (2013): Diseases of Poultry 13th edn. 2013;(Chapter 10, pp. 333–350.

Tripathy DN, Reed WM (2008): Pox. In Diseases of Poultry. 12th edition. Edited by Saif YM, Fadly AM, Glisson JR, Nolan LK, Swayne DE. Ames, Iowa: Blackwell Pub. Professional; 2008;291–307

Van Riper C III., Forrester DJ (2007): Avian Pox. In Infectious and parasitic diseases of wild birds, N. Thomas, B. Hunter and C. A. Atkinson (eds.). Blackwell Publishing Professional, Ames, Iowa. pp. 2007; 131–176.

Wagner EK, Hewlett MJ, Bloom DC, et al. (2008): Basic virology. Blackwell Pub., Malden, MA, pp. 2008;97–137, 359–379, 435–495.

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