Detection of HIV-1 CRF35_AD among Multiple Strains of Circulating HIV-1 Subtypes in Ibadan, Nigeria

• O.D Olaleye

Molecular analyses of Human Immunodeficiency Virus (HIV) in Nigeria have shown the presence of multiple strains of the virus in circulation with ongoing virus diversification over time. This study was carried out to determine the circulating HIV-1 strains, and their year to year changes over a three-year period. Blood samples were collected from 85 consenting HIV-1 infected anti-retroviral therapy-naïve patients. They include 42 males and 43 females with median age of 37 years (range 18-58 years) who presented at a voluntary counseling and testing centre in the University College Hospital, Ibadan. The env C2-V3 region of HIV-1 from the genomic DNA extract was amplified by nested PCR using primers WT1 and WT2, and KK40 and KK30 for the first and second rounds, respectively, and directly sequenced in both forward and reverse directions, edited and analysed for phylogenetic relationships by ClustalW and Maximum Likelihood methods in MEGA 5.03 software based on their nucleotide sequences. The target region was successfully sequenced from 64 HIV positive patients and identified as subtype A (3.13%), G (53.13%), CRF02_AG (28.13%), CRF06_cpx (14.06%), and CRF35_AD (1.56%) with variation noticed in subtype distribution over the three years of the study.

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