Prevalence of Extended Spectrum Beta-Lactamases Producing Enterobacteriaceae in Sheep and Goats from Selected Markets in Ibadan, Oyo-State, Nigeria.

Authors

  • F.E. Adetunji
  • E.A. Amosun
  • I.O. Olatoye
  • O.E. Ojo

Keywords:

Caprine, Ovine, Escherichia coli, Enterobacteriaceae, Extended Spectrum Beta Lactamase

Abstract

Antimicrobial misuse, unhygienic husbandry practices, close interaction between humans and animal as obtained in livestock market facilitate the emergence, dissemination and transmission of resistant Enterobacteriaceae. These organisms are responsible for various intestinal and extra-intestinal infections in human and animals. According to this report, the prevalence of Extended Spectrum Beta-Lactamase (ESBL)-producing Enterobacteriaceae in sheep and goats from selected markets in Ibadan.  Three hundred and four (304) samples were collected for a cross-sectional survey among the sheep and goat markets in Ibadan. From the same sheep and goats, 152 milk samples and 152 faeces samples were collected. In order to identify any antibiotic-resistant Enterobacteriaceae, these samples underwent bacteriological analysis. On MacConkey agar plates with 1 mg/L cefotaxime added, all samples were cultivated. Utilizing a biochemical test kit (Oxoid Microbact GNB 24E®), the isolates were identified. ESBL products were evaluated utilizing a double disc diffusion test with discs impregnated with cefpodoxime and cefpodoxime-clavulanic acid. By using the disc diffusion approach, antibiotic resistance was identified. Data was analysed using descriptive statistics. Eighty-eight ampicillin resistant Enterobacteriaceae strains were isolated from 304 samples collected and 23 (26.1%) of the isolates were cefotaxime- resistant Enterobacteriaceae isolates. Only 9(10.2%) were confirmed phenotypic ESBL-producers and they were all from faeces. All ESBL-producing Enterobacteriaceae were E. coli strain and showed 100% resistance to tetracycline and ceftazidime, 77.8% resistance to amoxicillin and sulphamethoxazole but susceptible to gentamycin and ciprofloxacin in this investigation. The ESBL-producing E. coli isolates showed different antibiotic resistance patterns. In this study, 100% of ESBL-producing E. coli were multidrug- resistant, showing resistance to at least three separate classes of antibiotics. Public awareness of the significance of stringent hygiene in animal husbandry needs to be raised in light of the possible threat that the existence of multidrug resistant ESBL-producing E. coli in small ruminants poses to public health.

Published

2023-10-31

Issue

Section

Infection/Immunology/Chemotherapy