Amelioration of thyroidectomy-induced glucose intolerance by Ocimum gratissimum leaf extract in male Wistar rat
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Keywords

Glucose intolerance
Hypothyroidism
Lipid profile
Oxidative stress
Rat

Abstract

Derailed glucose homeostasis is well documented in hypothyroidism and studies have found Ocimum gratissimum leaf extract (OG) to be beneficial in other conditions such as diabetes mellitus and metabolic syndrome characterized by imbalance glucose homeostasis. There is however dearth of information on the effect of OG in hypothyroid condition. This study therefore investigated the effect of OG on glucose metabolism in thyroidectomy-induced hypothyroid male Wistar rats.

Thirty-six male Wistar rats were randomly divided into six (6) groups (n=6) as 1 = control, 2 = control + OG, 3 =sham operated (Sham), 4 = Sham + OG, 5 = Thyroidectomized (Thyrd) and 6 = Thyrd + OG. Groups 1, 3 and 5 received 0.2 mL/kg of distilled water while 100 mg/kg of OG was administered to groups 2, 4 and 6 for 28 days post healing of the surgical site. Oral Glucose Tolerance Test was carried out, lactate level, lipid profile and markers of oxidative stress were assessed in the plasma while liver and muscle samples were obtained for the determination of glycogen content and hepatic hexokinase activities. Glucose intolerance was observed with increased AUC (18274 ± 442.55 mg.min/dL) in Group 5 and reversed in group 6 (15228±184.97 mg. min/dL) relative to control (14421±515.58 mg.min/dl). Hepatic hexokinase activity and plasma lactate level were significantly reduced in group 5.  Muscle glycogen decreased in groups 2, 4, 5 and 6 while hepatic glycogen increased in groups 3, 5 and 6. Triglycerides and HDL decreased while LDL increased in group 5 and were reversed in group 6. Malondialdehyde level was increased, and catalase activity decreased in group 5 while they were reversed in group 6. Findings from this study suggest that OG may prevent suppression of hepatic hexokinase activity and thus promote glucose utilization in thyroidectomized male Wistar rats

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