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Abstract
The objective of this study was to determine the effect of administration of tamsulosin on oral glucose tolerance in normal Wistar rats. Forty (40) male albino Wistar rats were selected and divided into four (4) groups of ten (10) rats each, viz, GROUP I, II, III and IV. Group I (Normal control): Distilled water (5ml/kg), Group II (Positive control): Carvedilol(800µg/kg), Group III (Tamsulosin treated): Tamsulosin (12µg/kg), Group IV (Tamsulosin treated): Tamsulosin (40µg/kg).Different treatments of Distilled water, Carvedilol and Tamsulosin were administered once every day orally for the period of six (6) weeks. After the 6th week of the study, all the treatments were withdrawn for a further 2 weeks (7th and 8th weeks). The Animals underwent 8 hours fasting. OGTT was done at baseline (0th), and then at3rd, 6th, 7thand 8th weeks. The blood glucose of all the animals was measured via tip tail incision at 0hours (pre-glucose load). Then, 2g/kg of D(+)-glucose powder dissolved in distilled water was administered to all the animals orally; after which blood samples were measured via tail tip incision at 30, 60 and 120 minutes using standard glucometer. ANOVA and Tukey Kramer post hoc test was used. The results were revealed therein. At the baseline of the study, 2nd, 3rd week, the groups of rats treated with carvedilol (positive control),tamsulosin high dose (40µg/kg) or low dose (12µg/kg) did not show any significant difference (P>0.05) in total area under the oral glucose tolerance curve compared to the normal control group and other inter group comparison. At the 6th week of the study, the group of rats treated with carvedilol (positive control), tamsulosin low dose (12µg/kg) and tamsulosin high dose (40µg/kg) revealed significantly higher values (P<0.05) of total area under the oral glucose tolerance curve compared to the normal control group. Other inter-group comparisons were not significantly different (P>0.05). At the 7th week of the study, the group of rats treated with carvedilol (positive control), tamsulosin low dose (12µg/kg) and tamsulosin high dose (40µg/kg) revealed no significant differences (P>0.05) in total area under the oral glucose tolerance curve compared to normal control group and other inter-groups comparison. At the 8th week of the study (two weeks after treatments withdrawal), only group of rats treated with carvedilol (positive control) revealed a significant higher values (P<0.05) of total area under the oral glucose tolerance curve than the normal control group. Other inter-group comparisons were not significantly different. The current study revealed that tamsulosin affects the glucose tolerance of the Wistar rats, thereby causing hyperglycemia
References
Ahrén, B., Lundquist, I., and Järhult, J. (2008). Effects of α1-, α2- and β-adrenoceptor blockers on insulin secretion in the rat. Acta Endocrinologica105 (1): 78–82.
Atanasov, A.G., Waltenberger, B., Pferschy-Wenzig, E.M., Linder, T., Wawrosch, C., and Uhrin, P. (2015). Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology Advances33 (8): 1582–1614.
Bilbis L.S., Muhammad, S.A., Saidu, Y., & Adamu, Y. (2012). Effect of vitamins A, C, and supplementation in the treatment of metabolic syndrome in albino rats .Biochemistry Research International, 1-7.
Boschmann, M., Krupp, G., Luft, F.C., Klaus, S., and Jordan, J. (2002). In Vivo Response to α 1 -Adrenoreceptor Stimulation in Human White Adipose Tissue. Obesity Research 10 (6): 555–558.
Cheng, J.T., Liu, I.M., Yen, S.T., & Chen, P.C. (2000). Role of alpha1A-adrenoceptor in the regulation of glucose uptake into white adipocyte of rats in vitro. Autonomic Neuroscience : Basic & Clinical 84 (3): 140–6.
Chika, A., Onyebuece, D.C., & Bello S.O. (2018). Phytochemical analysis and evaluation of antidiabetic effects in alloxan-induced diabetic rats treated with aqueous leaf extract of Acanthospermum hispidium. African Research Journal of Biomedical research 21:81-85.
Dikko, M., Bello, S.O., Chika, A., Mungadi I.A, Sarkingobir Y, & Aliyu, S. (2020a). Determination of Oral Glucose Tolerance (OGT) of Benign Prostatic Hyperplasia Patients Treated with Tamsulosin in Sokoto State, Nigeria. Nigerian Journal of Pharmaceutical and Applied Science Research, 9(2): 33-39.
Dikko, M. (2019). Exploration of gross effect of tamsulosin on glucose and insulin kinetics in ratsand humans. A PhD thesis submitted to the Postgraduate School Usmanu Danfodiyo University Sokoto, Nigeria.
Dikko, M., Bello, S.O., Chika, A, Mungadi, I.A., Sarkingobir, Y., & Umar, AI (2020b). Effect of Tamsulosin use on plasma insulin status in Benign Prostatic Hyperplasia patients in Sokoto, Nigeria. Journal of Applied Science and Environmental Management, 24 (4) 543- 548.
Flechtner-Mors, M., Jenkinson, C.P., Alt, A., Biesalski, H.K., Adler, G., & Ditschuneit, H.H. (2004). Sympathetic Regulation of Glucose Uptake by the α 1 -Adrenoceptor in Human Obesity. Obesity Research 12 (4): 612–620.
Jacob, S., Rett, K., Wicklmayr, M., Agrawal, B., Augustin, H.J., & Dietze, G.J. (1996). Differential effect of chronic treatment with two beta-blocking agents on insulin sensitivity: The carvedilol-metoprolol study. Journal of Hypertension14(4): 489–494.
Jerry, J. (2018). Glucose tolerance testing. Www.medicine.medscape.con. Retrieved 7/5/2020
Onakpoya, I.J., Heneghan, C.J., & Aronson, J.K. (2016). Post-marketing withdrawal of 462 medicinal products because of adverse drug reactions: A systematic review of the world literature. BioMed Central Medicine14 (1): 10.
Patrick, J.P. (2012). Oral glucose tolerance testing. Australian Family Physician, 41(6):391-193.
Preissner, R., Gohlke, B.-O., Drwal, M.N., Nickel, J., Omieczynski, C., & Siramshetty, V.B. (2015). WITHDRAWN—a resource for withdrawn and discontinued drugs. Nucleic Acids Research 44 (D1): D1080–D1086.
Satin, L.S. (2000). Localized calcium influx in pancreatic β-cells: Its significance for Ca2+-dependent insulin secretion from islets of Langerhans. Endocrine13 (3): 251–262.
Shivaprasad, G.M., Bharatha, A., Naikwadi, A.A., & Wali R.S. (2015). Effect of tamsulosin a selective α1 -antagonist on glucose homeostasis in rats. International Journal of Pharmacy and Pharmaceutical Sciences 7 (3): 232–234.
Suresha, R.N., Ashwini, V., Pragathi, B., Kalabharathi, H.L., Satish, A.M., & Pushpa, V.H. (2013). The effect of carvedilol on blood glucose levels in normal albino rats. Journal of Clinical and Diagnostic Research 7 (9): 1900–1903.
Uduala, T., Umar R.A., Isah R.A., Bello, M., Aiyelabegan, Isa, L.O., & Oduala G.B. (2018). Use of Gliricidia sepium aqueous leaf extracts as an antisickling agent: Oxidative stress biomarkers in wistar rats exposed to the extract. International Journal of Medical and Health Research4(8):79-83.
Umar, R. A., Hassan, S.W., Ladan, M.J., Matazu, I.K., Shehu, B., Shehu, R.A., Muhammed, L.G., & Molabo, F.I. (2010). Adverse effect associated with administration of antiretroviral drugs (Nevirapine, Lamivudine and Stavudine) to albino rats: Implication for management of patients with HIV/AIDS. Asian Journal of Biochemistry 5(3): 181-187.
Umar, M.T., Bello, S.O., Chika, A., & Oche, O.M. (2016). Attitude of nurses and pharmacists on adverse drug reactions reporting in selected hospitals in Sokoto. Journal of Research in Pharmacy Practice 5:219-2121.
United States National Library of Medicine (2020). Glucose tolerance test: Non pregnant .www.medlineplus.gov. Retrieved 7/5/2020
Woodcock, J. (2016). “Precision” drug development? Clinical Pharmacology & Therapeutics99 (2): 152–154.
World Health Organisation (2005). WHO Draft Guidelines for Adverse Event Reporting and Learning Systems. Geneva, Switzerland: Author. Report No. 80: Retrieved March 16, 2019.
World Health Organisation (2006). Definition and diagnosis of diabetes mellitus and intermediate hyperglycemia. WHO, 20 Avenue Appia, 121 Geneva 27,Switzerland.
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