Inhibition of alpha-Amylase and alpha-Glucosidase Activities by 2-Hydroxy-1,4-Naphthoquinone

Authors

  • Yusuf Iyanda Kwara State University
  • Sulyman, O. A.
  • Raliat Aladodo

DOI:

https://doi.org/10.54548/njps.v39i1.18

Abstract

The purpose of the present study was to investigate the ????-amylase and ????-glucosidase inhibitory potentials of 2-hydroxy-1,4-naphthoquinone (2HNQ). The inhibition of these two carbohydrates metabolizing enzyme was done by varying the concentrations of 2HNQ in the presence of α-amylase, α-glucosidase with starch and p-nitrophenylglucopyranoside (pNPG) respectively as their susbtrates. The mode of inhibitions of the two enzymes by 2HNQ was determined using double-reciprocal transformation. The result obtained indicated that 2HNQ inhibited ????-glucosidase activity with an IC50 0.260 mg/mL lowered than acarbose (1.530 mg/mL). Meanwhile, a moderate inhibitory potential of 2HNQ against ????-amylase was observed with an IC50 of 1.757 mg/mL compared with acarbose (IC50 of 3.600 mg/mL). Furthermore, 2HNQ amazingly mops up reactive oxygen species. The observed inhibitions of α-amylase and α-glucosidase activity as well as radical scavenging potentials of 2HNQ suggest that it may be a potential target for the management of diabetes mellitus.

References

Balogun, A

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Published

2024-06-30

Issue

Section

Full Length Research Articles

How to Cite

Inhibition of alpha-Amylase and alpha-Glucosidase Activities by 2-Hydroxy-1,4-Naphthoquinone. (2024). Nigerian Journal of Physiological Sciences, 39(1), 149-155. https://doi.org/10.54548/njps.v39i1.18