Anti-inflammatory Effect of the Alkaloid-rich Fraction of Landolphia owarensis

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N. N. IBEKWE
L. B. JOHN-AFRICA

Abstract

Background: Plants have several chemical compounds acclaimed to be responsible for the pharmacological actions produced when herbal products are administered to biological systems.


Objectives: This study was designed to investigate the anti-inflammatory effect of the alkaloid-rich fraction of the ethanol leaf extract of Landolphia owariensis.


Methods: Qualitative phytochemical analyses were carried on the crude extract using standard methods. The alkaloid-rich fraction was obtained from the crude ethanol extract, using the classical acid/base shake-up method and the obtained fraction tested positive to Dragendorf’s reagent. Oral acute toxicity was evaluated by OECD method (No 423). Anti-inflammatory effect of the fraction was evaluated using xylene-induce ear oedema and carrageenan-induced paw inflammation in mice at doses of 100, 200 and 400 mg/kg.


Results: Phytochemical screening revealed presence of alkaloids, flavonoids, tannins, saponins, steroids/terpenes and glycosides. Acute toxicity studies showed no adverse symptoms of toxicity during the 14-day observation period and no mortality was recorded, thus the LD50 was estimated to be greater than 2000 mg/kg. The alkaloid-rich fraction dose-dependently inhibited inflammation induced by xylene and carrageenan. In the xylene test, the fraction produced significant inhibition of 41.70 % at 400 mg/kg (p ≤ 0.05) while in the carrageenan test 55.69 % significant inhibition (p ≤ 0.001) was recorded with 400 mg/kg at 60 mins after induction of inflammation.


Conclusion: This study showed the anti-inflammatory potentials of the alkaloid-rich fraction of Landophia owariensis.

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How to Cite
IBEKWE, N. N., & JOHN-AFRICA, L. B. (2021). Anti-inflammatory Effect of the Alkaloid-rich Fraction of Landolphia owarensis. Nigerian Journal of Pharmaceutical Research, 17(1), 39–44. https://doi.org/10.4314/njpr.v17i1.4
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