Background: Electrolyte imbalance and oxidative stress (OS) are known to impair physiological functions, which can alter health and wellbeing. The reactive species produced due to OS are detoxified by endogenous antioxidants to maintain homeostasis. This study investigated the electrolyte and oxidative stress profile of a healthy adult population in Zaria, Nigeria and their relationship with experimental pain outcome. Method: Participants were apparently healthy adult volunteers between the ages of 20 to 65 years and drawn from the city of Zaria and its environs. Experimental pain was induced using pressure algometry. About 5 ml of blood was collected for determination of serum electrolytes, malondialdehyde (MDA), reduced glutathione (GSH) and superoxide dismutase (SOD). Result: The results showed that serum concentrations of sodium, potassium and chloride as well as oxidative stress profile did not vary with sex, age and ethnicity among the studied population. There was a significant negative correlation between pressure pain threshold and serum concentration of potassium (r = 0.2330, p = 0.003) and chloride (r = 0.2126, p = 0.007), while serum sodium correlated positively (r = 0.3439, p = 0.000). Serum MDA, SOD and GSH did not show statistically significant correlation with pressure pain threshold (p > 0.05). Conclusion: Serum electrolytes, but not oxidative stress markers, correlate significantly with experimental pressure pain threshold among healthy adult population in Zaria, Nigeria.