Resum
This study was conducted to find out the ability of Protein A obtained from a local isolate of Staphylococcus aureus to protect rats against infection by pathogenic organisms. A good amount
(5000µg) of Protein A was extracted from a small quantity (approximately 40g) of Staphylococcus aureus culture using lysostaphin technique. This extract was found to have protective property against pathogenic Escherichia coli and Pseudomonas aeruginosa in rats even at a low concentration of 50µg. The crude Protein A extract also compared favourably with imported standard Protein A in the study.
Keywords: Staphylococcus aureus, Protein A extract, pathogenic organisms.
Résumé
Cette étude était conduite pour rechercher l’habilité de la protéine A obtenue d’un isolat local de Staphylocoque aureus á protéger les rats contre l’infection des microorganismes pathogéniques. Une
quantité importante (5000µg) de Protéine A était extraite de 40g de culture de Staphylocoque aureus utiisant la technique de lysostaphine. Cet extrait démontrait une propriété protectrice contre Escherichia coli et Pseudomonas aeruginose pathogénique chez les rats a la concentration faible de 50µg. L’extrait pure de protéine A était favorablement comparablea la protéine A standard importee
Correspondence: Dr. Adeyeye J. Oke, Department of Microbiology, Baptist Medical Centre, P.O. Box 15, Ogbomoso, Nigeria. Email: adefolaames@yahoo.com
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