Abstract
Background: Use of saliva as alternative to blood in monitoring systemic diseases is still subject to continued research. Hence, this study evaluated changes in biochemical composition of saliva and plasma before and after hemodialysis and also determined the correlation between these factors in saliva and plasma of patients with chronic kidney disease (CKD).
Methods: A cross sectional study that included 50 patients with CKD undergoing hemodialysis. Whole saliva and blood samples were collected from the participants before and after dialysis. Samples were analyzed for urea, creatinine, total protein, sodium, potassium, calcium, chloride, and bicarbonate. Data were compared using Related Samples Wilcoxon Signed Rank test. Correlation between plasma and salivary parameters was determined using Spearman’s correlation test.
Results: Levels of salivary urea and creatinine were reduced in the post dialysis state in consistence with reduced plasma levels. Salivary and plasma bicarbonate levels were elevated in the post dialysis state compared to pre-dialysis while both salivary and plasma levels of total protein, sodium, potassium, calcium and chloride did not show significant change. There were positive correlations between salivary and plasma creatinine and potassium in the post dialysis state as well as calcium in both pre and post dialysis states.
Conclusion: Findings of this study suggest that saliva reflects plasma levels of biochemical factors in patients with CKD in the pre and post dialysis states. Hence, saliva may be an alternative to blood in monitoring patients with CKD undergoing dialysis.
Keywords: Saliva; blood; chronic kidney disease; dialysis
Résumé
Contexte: L’utilisation de la salive comme alternative au sang dans la surveillance des maladies systémiques fait toujours l’objet de recherches continuées. Par conséquent, cette étude a évalué les
changements dans la composition biochimique de la salive et du plasma avant et après l’hémodialyse et a également déterminé la corrélation entre ces facteurs dans la salive et le plasma des patients atteints d’insuffisance rénale chronique (IRC).
Méthodes : Une étude transversale qui a inclus 50 patients atteints d’IRC sous hémodialyse. Des échantillons entiers de salive et de sang ont été prélevés chez les participants avant et après la dialyse. Les échantillons ont été analysés pour l’urée, la créatinine, les protéines totales, le sodium, le potassium, le calcium, le chlorure et le bicarbonate. Les données ont été comparées à l’aide du test du Rang Signé Wilcoxon des échantillons reliés. La corrélation entre les paramètres plasmatiques et salivaires a été déterminée en utilisant le test de corrélation de Spearman.
Résultats : Les niveaux d’urée salivaire et de créatinine ont été réduits dans l’état post dialyse en accord avec des taux plasmatiques réduits. Les taux salivaires et bicarbonatés plasmatiques étaient élevés dans l’état post dialyse par rapport à la pré-dialyse, tandis que les taux salivaires et plasmatiques de protéines totales, de sodium, de potassium, de calcium et de chlorure n’ont pas montré de changement significatif. Il y avait des corrélations positives entre la créatinine salivaire et plasmatique et le potassium dans l’état post dialyse ainsi que le calcium dans les états de dialyse pré et post.
Conclusion : Les résultats de cette étude suggèrent que la salive reflète les taux plasmatiques de facteurs biochimiques chez les patients atteints d’IRC avant et après la dialyse. Par conséquent, la salive peut être une alternative au sang dans la surveillance des patients atteints d’IRC subissant une dialyse.
Mots-clés: Salive; sang; maladie rénale chronique; dialyse
Correspondence: Dr. Taye J. Lasisi, Department of Physiology and Oral Pathology, College of Medicine, University of Ibadan, Ibadan, Nigeria. E-mail: jameelahlasisi@yahoo.com
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