Assessment of bacterial contamination was carried out on ready to eat fruits and vegetable sold in Oja-Oba Market, Ilorin. Nine (9) samples of two (2) different fruits (watermelon and pawpaw) and vegetable (carrot) were collected randomly from different stationery vendors. Samples were analyzed to study the total bacterial count, total coliform count, total Salmonella count and total Staphylococcus count using 10-fold serial dilution and plate count method. Pure colonies were isolated using streak plate method and subjected to biochemical tests using standard procedures. Ten (10) antibiotics were used for susceptibility test against the bacterial isolates using disk diffusion method. Mean microbial load ranged from 60.17 ± 3.10 x 104 - 158.67 ± 6.90 x 104 cfu/ml for vendor A; 61.83 ± 2.60 x 104 - 144.33 ± 4.24 x 104 cfu /ml for vendor B and 56.83 ± 3.53 x 104 – 88.50 ± 3.10 x 104 cfu/ml for vendor C. Total coliform count ranged from 7.80 ± 1.10 x 104 – 26.70 ± 2.82 x 104 cfu/ml, total Staphylococcus count ranged from 5.00 ± 0.24 x 104 – 21.17 ± 3.06 x 104 cfu/ml and total Salmonella count ranged 8.84 ± 1.18 x 104 - 11.67 ± 1.41 x 104 cfu/ml. Antibiotics susceptibility test ranged from10.00 ± 0.00 – 26.50 ± 0.21 mm. Ciprofloxacin and gentamycin had an average diameter of zone of inhibition at 24.50 ± 0.71 and 26.50 ± 0.21 mm respectively. It can be concluded that ready to eat fruits and vegetables sold in Oja-Oba Market contain considerable numbers of pathogenic bacteria
Abakari, G. and Cobbina, S.J. (2018). Microbial quality of ready to eat vegetable salads vended in the central business district of Tamale, Ghana. International Journal of Food Contamination. 5:3.
Abiola, C. and Oyetayo, V.O. (2016). Isolation and Biochemical characterization of Microogranism associated with fermentation of Kestings Groundnut (Macrotyloma geocarpum). Reserch Journal of microbiology. 11(2-3): 47-55.
Adams, M.R. and Moss, M.O. (2018). Bacterial agents of foodborne illness. Food Microbiology. 182–268.
Adebolu, T.T. and Ifesan, B.O. (2019). Bacteriological quality of vegetables used in salads. Nigerian Journal of Microbiology. 15(1):81-85.
Akusu, O.M., Kiin-Kabari, D.B. and Wemedo, S.A. (2016). Microbiological quality of selected street vended foods in Port Harcourt metropolis, rivers state, Nigeria. Sky Journal of Food Science. 5(2):008–11.
Altieri, M.A. and Nicholls, C.I. (2017). The adaptation and mitigation potential of traditional agriculture in a changing climate. Climate Change. 140(1): 33-45.
Amissah, A. and Owusu, J. (2012). Assessing the microbiological quality of foods sold on and around Koriforidua polytechnic campus in Ghana. Annals Food Science and Technology. 13(1):60–67.
Aune, D., Giovannucci, E., Boffeta, P., Fadnes, L.T., Keurn, N., Norat, T., Greenwood, D.C., Riboli, E., Vatten, L.J. and Tonstad, S. (2017). Fruits and vegetable intake and the risk of cardiovascular disease- total cancer and all cause mortality-a systematic review and dose response meta-analysis of prospective studies. Journal of Medical Research: 46(3): 1029-1056.
Bakobie, N., Duwiejuah, A., Cobbina, S. and Miniyila, S. (2017). Microbial profile of common spices and spice blendsused in Tamale, Gana. International Journal of Food Contamination. 4:10.
Berg, A., De Oliveira, M.A., De Souza, V.M. and De Martinis, E. (2014). Microbial quality of ready-to-eat minimally processes vegetables consumed in Brazil. Journal of Food Control. 22(8):1400–1403
Boeing, H., Bechthold, A., Bub, A., Ellinger, S., Haller, D., Kroke, A., leschick-Bonnet, E., Muller, M.J., Oberritter, H., Schulze, M. Peter, S. and Watzl, B. (2012). Vegetables and Fruits in the prevention of chronic diseases. European Journal of Nutrition. 51(6): 637-663.
Chester, R. and Copper, J. (2019).Citrate utilization test. Microbiology Laboratory. BIOL 3702: 1-3.
Clinical and Laboratory Standards Institute (2009). Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - Tenth Edition. 556-557
CLSI. (2017). Performance standards for antimicrobial susceptibility testing. Information supplement. 17: 44-52.
Food and Drug Board (FDB). (2013). “Salmonellosis.” Division of Foodborne, Bacterial, and Mycotic Diseases. Department of Health and Human Services. 1-55.
Foster, T.J. and McDevitt, D. (2013). Molecular basis of adherence of Staphylococci to biomaterials. American society for Microbiology. 2: 31.
Francis, G.A., Gallone, A., Nychas, G.J., Sofos, J.N., Colelli, G., Amodo, M.L. and Spano, G. (2012). Factors affecting quality and safety of fresh-cut produce. Critical Reviews in Science and Nutrition. 52: 595-610.
Getie M., Abebe, W. and Tessema, B. (2019). Prevalence of enteric bacteria and their antimicrobial susceptibility patterns among food handlers in Gonder town Northwest Ethiopia. Journal of Antimicrobial Resistances and Infection Control. 8:111
Health Protection Agency. (2009). Guidelines for Assessing the Microbiological Safety of Ready-to-Eat Foods Placed on the Market. USA. 1-12.
Karoki, W.H., Karanga, D.N., Bebora, L.C. and Njagi, L.W. (2018). Isolation Characterization and quantification of bacterial from African sausages sold in Nairobi. International Journal of Food Science. 38(61): 55-65.
Kaur, P. and Rai, N. (2015). Bacteriological analysis of fresh vegetables from main market of Dehradun. International Journal of Pharmaceutical Tech Research. 8(3): 415-425.
Khan, Z.A., Sidiqui, M.F. and Park, S. (2019). Current emerging methods of Antibiotic susceptibility testing. Diagnostics. 9(2): 49
Liu, R.H. (2013). Health-promoting components of fruits and vegetables in the diet. Advance in Nutrition. 4(3):384–392.
Mahon, C.R., Lehman, D.C. and Manuselos, G. (2011). Textbook of diagnostic microbiology. 4th Ed. 156-161.
Mashak, Z., Langoodi, A.M., Ehsani, A., Fathabad, A.E. and IIkhanipoor, A. (2015). Microbiological quality of ready-to-eat foods of Tehran Province. African Journal of Food Science. 9(5): 257–61.
McAdow, M., Missiakas, D.M. and Schnee, W. (2012). Staphylococcus aureus secrets coagulase and von willebrand factor binding protein to modify the coagulation cascade and establish host injection. Journal of immune. 4:141-148.
McDevitt, S. (2009). Methylred and voges-proskauer test procedures. American society for microbiology. 731(12): 234-236.
Mritunjay, S.K. and Kumar, V. (2015). Fresh farm produce as a source of Pathogens. Research Journal of Environmental Toxicology: 9:59-70.
Odu, N.N. and Assor, P. (2013). Microbiological analysis of ready to eat food (cooked rice and beans) sold among different restaurant in University of Port Harcourt Nigeria. Journal of Academia Arena. 5(1):62-66.
Oje, O.J., Ajibade, V.A., Fajilade, O.T. and Ajenifuja, O.A. (2018). Microbiological analysis of ready to eat foods vended in mobile outlet catering units from Nigeria. Journal of advances in Food science and Technology. 5(1): 15-19.
Olawale, A.K., Salako, R.J., Olawale, O. A. and Famurewa, O. (2015). Antibiotic resistance Enterococcus feacalis isolated from cateens in Osun State Nigeria. British Microbiology Research Journal. 6 (4): 196-206
Orji, J.O., Ayoku, T.E., Nnachi, A.U., Obaji, M. and Oke, C.O. (2017). Bacteriological quality of mixed fruits/vegetables salads and selected ready to eat vegetables sold in Abakaliki Metropolis. World Journal of Pharmaceutical and Life Science. 3(3): 157-163.
Qadri, O.V., Yousouf, B. and Srivastava, A.K. (2015). Fresh-cut fruits and vegetables: Critical factors influencing microbiology and novel approaches to prevent microbial risks. Journal of Cogent food and Agriculture. 1(1): 1-26.
Sharff, R.L. (2012). Economic burden from health losses due to food borne illness in the United States. Journal of Food protection. 75(1): 123-31.
Shields, P., Cathcart, L. and Wang, J.I. (2013). Oxidase Test protocols. Journal of Microbiology. (15):1-10.
Temgoua, E., Ntangmo, T.H. and Njine, T. (2015). Vegetable production systems of swamp Zone in Urban Environment in West Cameroon: case of Dschang City. University Journal of Environmental Research and Technology. 2(2):83–92.
Tiso, M., Tejero, J., Kenney, C.T., Frizzell, S. and Gladwin, M.T. (2012). Nitrite reductase activity of nonsymbiotic heamoglobins from Arabidopsis thaliana. Biochemistry. 51(26): 5285-5292.
WHO. (2015). Assuring Food Safety and Quality: Guidelines for Strengthening National Food Control Systems. Food and Nutrition Paper. 112:76.
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